[1]秦士维,夏秀英,安利佳.蓝莓果实潜伏侵染病原真菌的分离与鉴定[J].北方园艺,2017,41(18):41-48.[doi:10.11937/bfyy.20170861]
　QIN Shiwei,XIA Xiuying,AN Lijia.Isolation and Identification of Latent Pathogenic Fungi in Blueberry Fruits[J].Northern Horticulture,2017,41(18):41-48.[doi:10.11937/bfyy.20170861]
点击复制

蓝莓果实潜伏侵染病原真菌的分离与鉴定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 41-48 栏目: 出版日期: 2017-09-30
Title:
Isolation and Identification of Latent Pathogenic Fungi in Blueberry Fruits
作者:
秦士维; 夏秀英; 安利佳
大连理工大学 生命科学与技术学院，辽宁 大连 116024
Author(s):
QIN Shiwei; XIA Xiuying; AN Lijia
School of life Science and Biotechnology，Dalian University of Technology，Dalian，Liaoning 116024
关键词:
蓝莓; 潜伏侵染; 病原真菌; 分离; 鉴定
Keywords:
blueberry; latent infection; pathogenic fungi; isolation; identification
DOI:
10.11937/bfyy.20170861
文献标志码:
A
摘要:
以辽宁省蓝莓鲜果为试材，采用组织分离法分离潜伏侵染果实的病原真菌，通过形态学和分子生物学方法，对病原菌进行分类鉴定，研究了真菌的潜伏侵染状况，为指导蓝莓果实病害防治提供参考依据。结果表明：辽宁省蓝莓果实普遍存在真菌潜伏侵染现象，不同品种带菌率存在差异，‘M7’带菌率较高，“布里吉塔” “爱国者” “奥尼尔”和“南好”带菌率较低。从8个蓝莓品种中共分离鉴定出9种潜伏病原真菌，分别为互隔交链孢霉（Alternaria alternata）、细极链格孢菌（Alternaria tenuissima）、芽枝霉属（Cladosporium sp.）、灰葡萄孢菌（Botrytis cinerea）、黑曲霉（Aspergillus niger）、嗜松青霉（Penicillium pinophilum）、茎点霉属（Phoma sp.）、毛壳属（Chatetomium sp.）和镰刀菌属（Fusarium sp.）。其中，互隔交链孢霉（Alternaria alternata）可在多个品种中分离，为蓝莓果实主要潜伏侵染病原真菌。另外，从蓝莓果实中分离到一株葡萄有孢汉逊酵母（Hanseniaspora uvarum），该酵母对互隔交链孢霉具有良好的抑制作用。
Abstract:
The occurrence of latent infection of pathogenic fungi in blueberry fruits from Liaoning Province was researched.The fresh blueberry fruits were used as materials，using the tissue isolation method，latent pathogenic fungal were isolated.Morphology and molecular biology were used to identify the fungal species in order to provide a theoretical basis for blueberry decay control.The results showed that the latent infection of fungi was commonly found in the healthy blueberry fruits，and there existed significant difference in the latent infection rates among different cultivars.The rate of latent infection in ‘M7’ was the highest as compared to other cultivars such as ‘Brigitta’ ‘Patriot’‘O′Neal’ and ‘Southgood’.Nine pathogenic strains (Alternaria alternata, Alternaria tenuissima, Cladosporium sp., Botrytis cinerea, Aspergillus niger，Penicillium pinophilum,Phoma sp., Chatetomium sp., Fusarium sp.) which could cause blueberry decay were identified.In addition, Alternaria alternata was the main latent infection fungi in blueberry fruits whereas Chatetomium sp.，a new pathogen of blueberry was reported for the first time.Hanseniaspora uvarum showed a strong antagonistic effect on Alternaria alternata.

参考文献/References:

[1]HKKINEN S H，KRENLAMPI S O，MARINA H，et al.Content of the flavones quartering，myricetin,and kaempferol in 25 edible berries[J].Journal of Agricultural and Food Chemistry,1999,47:2274-2279.

[2]WILLIAMS C M，MOHSEN M A，VAUZOUR D，et al.Blueberry-induced changes in spatial working memory correlate with changes in hippocampal CREB phosphorylation and brain-derived neurotrophic factor (BDNF)levels[J].Free Radical Biology and Medicine,2008,45(3):295-305.
[3]DEL B C，MARTINI D，VENDRAME S，et al.Improvement of lymphocyte resistance against H2O2-induced DNA damage in Sprague-Dawley rats after eight weeks of a wild blueberry(Vaccinium angustifoliurn)-enriched diet[J].Mutation Research Genetic Toxicology and Environmental Mutagenesis,2010,703(2):158-162.
[4]NETO C C.Cranberry and blueberry:Evidence for protective effects against cancer and vascular diseases[J].Molecular Nutrition and Food Research,2007,51(6):652-664.[5]李亚东,孙海悦,陈丽.我国蓝莓产业发展报告[J].中国果树,2016(5):1-10.
[6]PRITTS M P，HANCOCK J F.High bush blueberry production guide[M].New York:Northeast Regional Agricultural Engineering Service,1992:4-7.
[7]JANISIEWICZ W，CONWAY W S.Combining biological control with physical and chemical treatments to control fruit decay after harvest[J].Stewart Postharvest Review,2010,6(1):1-16.
[8]李传道,周仲铭,鞠国柱.森林病理学通论[M].北京:中国林业出版社,1985:123-125.
[9]REDLIN S C，CARRIS L M.Endophytic fungi in grasses and woody plants systematics，ecology，and evolution[M].MN:American Phytopathological Society,1996:3-31.
[10]GOSCH C.Mummy berry disease (Monilinia vaccinii-corymbosi) on highbush blueberries in Europe[J].International Society for Horticultural Science,2006,715:469-472.
[11]FARR D F.Morphological and molecular characterization of Phomopsis vaccinii and additional isolates of Phomopsis from blueberry and cranberry in the eastern United States[J].Mycologia,2002,94(3):494-504.
[12]KWON J H,CHOI O.First report of Botrytis cinerea as a postharvest pathogen of blueberry in Korea[J].Mycobiology,2011,39(1):52-53.
[13]MARIANA V G,PATRIARCA A,TERMINIELLO L,et al.Toxigenic Alternaria species from Argentinean blueberries[J].International Journal of Food Microbiology,2012,154(3):187-191.
[14]JAMES J P，MARK K E，ALLAN W S，et al.Anthracnose fruit rot resistance in blueberry cultivars[J].Plant Disease,2005,89(1):33-38.
[15]THOMAS T.Influence of relative virulence and latent infections on the development of Monilinia to Greek peach orchards[J].Crop Protection,2017,94:159-165.
[16]PEARSON R C，HALL D H.Factors affecting the occurrence and severity of blackmold of ripe tomato fruit caused by Altenaria alternata[J].Phytopathology,1975，65:1352-1359.
[17]WILLIAMSON P M，SIVASITHAMPARAM K，COWLING W A.Formation of subcuticular corallloid hyphae by Phomopsis leptostro-miformis upon latent infection of narrow-leafed lupins[J].Plant Disease,1991,75:1023-1026.
[18]周笑犁,王瑞,雷霁卿,等.蓝莓采后病原真菌分离及其生物学鉴定[J].食品科技,2015,40(9):283-293.
[19]胡美姣,高兆银,李敏.杧果果实潜伏侵染真菌种类研究[J].果树学报,2012,29(1):105-110.
[20]宋聪,黄亚丽,谢晨星.河北省冬枣黑斑病病原菌的分离鉴定及生物防治初探[J].微生物学杂志,2016,36(5):85-89.
[21]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979:501-547.
[22]陆家云.真菌病害诊断[M].2版.北京:中国农业出版社,1997.
[23]NOVICKI T J，LAFE K，BUI L.Genetic diversity among clinical isolates of Acremonium strictum determined during an investigation of a fatal mycosis[J].Journal of Clinical Microbiology,2003,41(6):2623-2628.
[24]TOLAINI V，ZJALIC S，REVERBERI M.Lentinula edodes enhances the biocontrol activity of Cryptococcus laurentii against Penicillium expansum contamination and patulin production in apple fruits international[J].Journal of Food Microbiology,2010,138:243-249.
[25]ZHU X Q，XIAO C L.Phylogenetic，morphological，and pathogenic characterization of Alternaria species associated with fruit rot of blueberry in California[J].Etiology,2015,105(12):1555-1567.
[26]雷桂林,刘云龙,刘雪峰.雪松针叶枯斑病病原鉴定及其生物学特性研究[J].林业科学研究,2003,24(5):69-72.
[27]SANZANI S M，SCHENA L，CICCO V D，et al.Early detection of Botrytis cinerea latent infections as a tool to improve postharvest quality of table grapes[J].Postharvest Biology and Technology,2012,68(6):64-71.
[28]许晴晴.香芹酚对蓝莓采后病害抑制和贮藏品质的影响研究[D].南京:南京农业大学,2014.
[29]VON A J A.The Ascomucete genus Chaetomium[M].Berlin:Crameer J,1986:1-162.
[30]QIN X J，XIAO H M，XUE C H，et al.Biocontrol of gray mold in grapes with the yeast Hanseniaspora uvarum alone and in combination with salicylic acid or sodium bicarbonate[J].Postharvest Biology and Technology,2015,100:160-167.
[31]BASHA H，RAMANUJAM B.Growth promotion effect of Pichia guilliermondii in chilli and biocontrol potential of Hanseniaspora uvarum against Colletotrichum capsici causing fruit rot[J].Biocontrol Science and Technology,2015,25(2):185-206.
[32]刘艳,叶建仁.植物病害潜伏侵染研究进展[J].南京林业大学学报,2000,24(5):69-72.
[33]刘秀娟,李继勇,杨业铜.海南岛芒果果实真菌潜伏侵染的研究[J].植物病理学报,1986,16(1):47-51.
[34]胡美姣,张令宏,杨凤珍.香蕉果实潜伏侵染真菌研究[J].中国果实,2004(5):6-8.
[35]张居念,林河通,谢联辉.龙眼果实潜伏性病原真菌的初步研究[J].热带作物学报,2006,7(4):78-82.
[36]PARIKKA P，LEMMETTY A.Tracing latent infection of Colletotrichum acutatum on strawberry by PCR[J].European Journal of Plant Pathology,2004,110:393-398.
[37]郭晓月,丁雅迪,邓佳.蓝莓贮藏期病原真菌的分离与鉴定[J].北方园艺,2015(24):104-108.
[38]梁晨,刘翠,赵洪海.蓝莓贮藏期病原菌的鉴定及病菌的温度效应[C]//彭友良,王宗华.中国植物病理学会第九届全国会员代表大会暨2010年学术年会.厦门:中国植物病理学会,2010:108-113.
[39]戴启东,李广旭,杨华.蓝莓采后病害的病原鉴定及发生规律的研究[J].果树学报,2016(10):1299-1306.
[40]RIVERA S A，ZOFFOLI J P，LATORRE B A.Infection risk and critical period for the postharvest control of gray mold(Botrytis cinerea) on blueberry in Chile[J].Plant Disease,2013,97(8):1069-1074.
[41]秦晓杰,高梦,蒋晓玲,等.采前喷施拮抗酵母菌对草莓采后贮藏性能的影响[J].南京农业大学学报,2015,38(1):152-160.

相似文献/References:

[1]王明洁,吴雨蹊,段亚东,等.蓝莓栽培土壤改良技术研究[J].北方园艺,2014,38(14):179.
　WANG Ming-jie,WU Yu-xi,DUAN Ya-dong,et al.Improvement Research of Technique on Cultivation Soil of Blueberry[J].Northern Horticulture,2014,38(18):179.
[2]王贺春.蓝莓冬季修剪技术[J].北方园艺,2013,37(13):58.
　WANG He-chun.Winter Pruning Techniqne of Blueberry[J].Northern Horticulture,2013,37(18):58.
[3]聂飞,张玉春.我国蓝莓产业发展的机遇与挑战[J].北方园艺,2014,38(04):165.
　NIE Fei,ZHANG Yu-chun.Opportunities and Challenges for the Development of Blueberry Industry in China[J].Northern Horticulture,2014,38(18):165.
[4]杨芩,任永权,廖优江,等.五个兔眼蓝莓品种有效可授期研究[J].北方园艺,2013,37(14):5.
　YANG Qin,REN Yong-quan,LIAO You-jiang,et al.Study on Effective Pollination Period of Five Cultivars of Rabbiteye Blueberry[J].Northern Horticulture,2013,37(18):5.
[5]傅 冰,洪 震,刘跃钧,等.蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析[J].北方园艺,2013,37(17):95.
　FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(18):95.
[6]张文英,周 婧.低糖蓝莓番茄复合果酱的研制[J].北方园艺,2013,37(17):142.
　Brewing Technology Engineering Research Center,Department of Food Science,Jilin Agricultural Science and Technology College,et al.Preparation of Low-sugar Jam with Blueberry and Tomato[J].Northern Horticulture,2013,37(18):142.
[7]龚 娜,肖 军,杨 镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
　GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(18):175.
[8]王贺春.适于丹东地区温室栽培的蓝莓引种与筛选[J].北方园艺,2013,37(12):33.
　WANG He-chun.Introduction and Selection of Blueberry Varieties for Greenhouse That Suitable for Dandong Region[J].Northern Horticulture,2013,37(18):33.
[9]吴立仁,段亚东,杜汉军,等.黑龙江省蓝莓种植应该注意的几个问题[J].北方园艺,2013,37(05):50.
　WU Li-ren,DUAN Ya-dong,DU Han-jun,et al.Several Problems Existing in Planting of Blueberry in Heilongjiang Province[J].Northern Horticulture,2013,37(18):50.
[10]刘 波.果胶酶对蓝莓果汁澄清效果的研究[J].北方园艺,2014,38(03):127.
　LIU Bo.Study on Clarification Effect of Pectinase on Blueberry Juice[J].Northern Horticulture,2014,38(18):127.

备注/Memo

第一作者简介：秦士维（1991-），男，硕士研究生，研究方向为植物真菌病害。E-mail:860758110@qq.com.

责任作者：夏秀英（1972-），女，博士，副教授，研究方向为植物生理与分子生物学。E-mail:xx47@dlut.edu.cn.
收稿日期：2017-04-11

更新日期/Last Update: 2017-10-01