LI Dahong,ZHAO Li,LI Zhen,et al.Effect of High Temperature Stress on Physiological Characteristics and POD，SOD Isoenzyme in Brassica oleracea Seedlings[J].Northern Horticulture,2015,39(19):6-10.[doi:10.11937/bfyy.201519002]
高温胁迫对不同品种羽衣甘蓝幼苗叶的生理特性及SOD、POD同工酶的影响
- Title:
- Effect of High Temperature Stress on Physiological Characteristics and POD，SOD Isoenzyme in Brassica oleracea Seedlings
- 文献标志码:
- A
- 摘要:
- 以4个不同品种的羽衣甘蓝(Brassica oleracea var.acephala)幼苗为试材，研究了在高温胁迫8 d(39±0.5)℃/(28±0.5)℃ (昼/夜)后，羽衣甘蓝幼苗叶片一些抗逆境的生理生化指标及SOD、POD同工酶变化，以探讨不同羽衣甘蓝植物对高温的适应性。结果表明：随胁迫时间的延长，不同品种的羽衣甘蓝的叶绿素含量显著下降，而幼苗叶MDA、脯氨酸含量与质膜透性含量增加；在处理后2 d，SOD、POD含量有较大幅度的升高，但第4天后开始出现下降，不同品种之间有显著差异；试验表明“名古屋”、“京冠红2号”比“红鸥”和“叶牡丹”有较强的耐热性；SOD、POD同工酶研究表明，“名古屋”和“叶牡丹”的SOD同工酶谱带具有相似性，而与“红鸥”与“京冠红2号”不同；“名古屋”和“京冠红2号”的POD同工酶谱带相似，而“叶牡丹”与“红鸥”的POD谱带相似，但“名古屋”和“京冠红2号”与“叶牡丹”和“红鸥”的POD谱带差异较大。
- Abstract:
- Four different regions of the kales(Brassica oleracea）seedlings were used as materials，treated at (39±0.5)℃/(28±0.5)℃ (day/night temperature) for 8 days，and several related physiological indexes and SOD，POD isoenzyme in kale seedlings were determined，in order to discuss their plant adaptability under high temperature conditions.The results showed that the relative electric conductivity content，contents of malondialdehyde(MDA)，and free proline were gradually increased，but chlorophyll content decreased with the time of high temperature continued in different varieties.During the early 2 day after treatment，superoxide dismutase(SOD) and peroxidase(POD) activities increased dramatically，while fourth days later，they began to decline.But there were significant differences among different varieties.Research data showed that ‘Nagoya’ and ‘Jingguan red 2’ had stronger heat resistance than the ‘Red gull’ and ‘Leaf peony’.According to the analysis of POD，SOD isoenzyme bands，the band of SOD isoenzyme in ‘Nagoya’ and ‘Leaf peony’ were similar，and ‘Red gull’ and ‘Jingguan red 2’ were different.The bands of POD isoenzyme were similar between ‘Nagoya’ and ‘Jingguan red 2’，while ‘Leaf peony’ and ‘Red gull’ POD bands were similar，the bands of POD isoenzyme were difference between ‘Nagoya’，‘Jingguan red 2’ and ‘Leaf peony’，‘Red gull’.
参考文献/References:
[1]ZAVORUEVA E N，USHAKOVA S A.Characteristics of slow induction curve of chlorophyll fluorescence and CO2 exchange for the assessment of plant heat tolerance at various levels of light intensity[J].Russ J Plant Physiol,2004，51:294-301. [2]孙中海,马湘涛.柑橘叶片细胞膜热稳定性的研究[J].华中农业大学学报,1999（18）:375-377. [3]赵华渊,李莹莹.羽衣甘蓝的观赏特性及园林应用[J].黑龙江农业科学，2011(2):96-97. [4]贾兰虹,张华艳,李长海.羽衣甘蓝苗期低温控制反季节栽培技术[J].北方园艺，2005(2):46-47. [5]高俊风.植物生理学实验指导[M].北京:高等教育出版社,2006. [6]陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2006. [7]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2011. [8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000. [9]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003. [10]罗广华,王爱国.植物SOD的凝胶电泳及活性的显示[J].植物生理学通讯,1983,19(6):44-45. [11]WAHID A，GELANI S，ASHRAF M,et al.Foolad Heat tolerance in plants:an overview Environ[J].Exp Bot,2007,61:199-223. [12]HSU Y T，KAO C H.Cadmium-induced oxidative damage in rice leaves is reduced by polyamines[J].Plant Soil,2007,291:27-37. [13]MISHRA Y，BHARGAVA P，RAI L C.Differential induction of enzymes and antioxidants of the antioxidative defense system in Anabaena doliolum exposed to heat stress[J].J Therm Biol,2005,30:524-531. [14]VYAS D，KUMAR S.Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis(L.) O Kuntze[J].Biochem Biophys Res Commun,2005,329:831-838. [15]NOCTOR G，FOYER C H.Ascorbate and glutathione：keeping active oxygen under control[J].Annu Rev of Plant Physiol and Plant Mol Biol,1998,49(4):246-279. [16]王爱国，罗广华，邵从本.大豆种子超氧物歧化酶的研究[J].植物生理学报,1983，9(1):77-79. [17]武宝玕,格林,托德.小麦幼苗中过氧化物歧化酶活性与幼苗脱水忍耐力相关性的研究[J].植物学报,1985,27(2):152-160.
相似文献/References:
[1]王振兴,艾 军,杨义明,等.不同温度对五味子叶片光系统 Ⅱ 活性的影响[J].北方园艺,2014,38(14):160.
WANG Zhen-xing,AI Jun,YANG Yi-ming,et al.Effect of Different Temperature Treatments on Photosystem Ⅱ in Leaves of Schisandra chinensis (Turcz.) Baill.[J].Northern Horticulture,2014,38(19):160.
[2]吴丽君,张俊凤.高温胁迫对番茄幼苗生长和生理指标的影响[J].北方园艺,2013,37(23):19.
WU Li-jun,ZHANG Jun-feng.Effect of High Temperature Stress on Growth and Physiological Indexes of Tomato Seedlings[J].Northern Horticulture,2013,37(19):19.
[3]范飞,李绍鹏,高新生,等.不同茄子品种幼苗耐热性比较研究[J].北方园艺,2013,37(15):15.
FAN Fei,LI Shao-peng,GAO Xin-sheng,et al.Comparative Study on the Heat Tolerance of Different Eggplant Varieties at Seedling Stage[J].Northern Horticulture,2013,37(19):15.
[4]朱鑫,沈火林.高温胁迫对芹菜幼苗细胞膜稳定性的影响[J].北方园艺,2014,38(07):15.
ZHU Xin,SHEN Huo-lin.Effect of High Temperature Stress on Cell Membrane in Celery Seedlings[J].Northern Horticulture,2014,38(19):15.
[5]祝朋芳,康耀海,黄娟娟,等.羽衣甘蓝SRAP体系建立与优化[J].北方园艺,2014,38(09):121.
ZHU Peng-fang,KANG Yao-hai,HUANG Juan-juan,et al.The Optimization of SRAP-PCR Amplification Program and System in Kale[J].Northern Horticulture,2014,38(19):121.
[6]李荣华,郭培国,张华,等.高温胁迫对不同耐热性菜心材料生理特性的差异研究[J].北方园艺,2013,37(01):1.
LI Rong-hua,GUO Pei-guo,ZHANG Hua,et al.Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage[J].Northern Horticulture,2013,37(19):1.
[7]李荣华,郭培国,张华,等.高温胁迫对不同耐热性菜心材料生理特性的差异研究[J].北方园艺,2012,36(01):1.
LI Rong-hua,GUO Pei-guo,ZHANG Hua,et al.Effects of Heat Stress on Several Physiological Traits in Heat-yolerant and Heat-Sensitive Genotypes of Flowering Chinese Cabbage[J].Northern Horticulture,2012,36(19):1.
[8]王文举,王振平.亚硒酸钠对“红地球”葡萄耐热性及果实品质的影响[J].北方园艺,2012,36(24):47.
WANG Wen-ju,WANG Zhen-ping.The Effect of Sodium Selenite on Heat-resistance and Berry Quality of Red Globe Grape[J].Northern Horticulture,2012,36(19):47.
[9]祝朋芳,房 霞,黄娟娟,等.粉色叶羽衣甘蓝花青素提取效应分析[J].北方园艺,2012,36(24):67.
ZHU Peng-fang,FANG Xia,HUANG Juan-juan,et al.Analysis of Extraction Effect on Anthocyanin of Pink Leaf Brassica oleracea var.acephala[J].Northern Horticulture,2012,36(19):67.
[10]王庆,刘安成,张瑞博,等.高温胁迫对多叶羽扇豆生长及生理指标的影响[J].北方园艺,2013,37(24):52.
WANG Qing,LIU An-cheng,ZHANG Rui-bo,et al.Effects of High Temperature Stress on Growth and Physiological Indices of Lupinus polyphlla Lindl.[J].Northern Horticulture,2013,37(19):52.
备注/Memo
第一作者简介：李大红（1968-），男，博士，副教授，现主要从事园林植物等研究工作。E-mail:moderatenlx@163.com.基金项目：河南省科技发展规划资助项目（112300410042）。