LIU Wei-wei,SONG Shu-min,PAN Jing,et al.Establishment of Regeneration System in the Rachis of Garlic[J].Northern Horticulture,2015,39(10):108-110.[doi:10.11937/bfyy.201510026]
大蒜花序轴离体再生体系的建立
- Title:
- Establishment of Regeneration System in the Rachis of Garlic
- 文章编号:
- 1001-0009（2015）10-0108-03
- Keywords:
- Allium sativum L.; rachis; regeneration system
- 分类号:
- S 633.403.6
- 文献标志码:
- A
- 摘要:
- 以大蒜花序轴为试材，采用植物组织离体培养方法，研究了不同激素、蔗糖浓度及培养基的种类对花序轴分化及继代培养的影响。结果表明:以MS为基本培养基诱导大蒜花序轴分化的最佳培养条件为MS+6-BA 1.5 mg/L+NAA 0.1 mg/L，30 g/L蔗糖，pH 5.8，分化率可达100%，增殖系数达46.3；最佳的继代培养基为1/2 MS+6-BA 0.1 mg/L+NAA 0.5 mg/L,成活率可达100%，且长势最强。
- Abstract:
- Taking rachis garlic as test material，using plant tissue culture method，the effect of different concentrations of cytokinin，sucrose concentration and culture medium type on rachis of garlic were studied.The results showed that induction differentiation of rachis was the best when the medium was MS containing 6-BA 1.5 mg/L，NAA 0.1 mg/L and sucrose 30 g/L at pH 5.8,the differentiation rate was 100%,the proliferation ratio was 46.3.The suitable subculture conditions were 1/2 MS medium containing 6-BA 0.1 mg/L，NAA 0.5 mg/L，subsisr rate was 100%,and grew strongly.
参考文献/References:
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备注/Memo
第一作者简介：刘伟伟（1982-），女，硕士，农艺师，现主要从事植物生物技术等研究工作。E-mail:lww8728@126.com.