CHEN Xing-xing,SHAO Xiu-li,HE Song-lin.Effect of Different Light Quality Ratios of LED on Growth of Spathiphyllum in vitro[J].Northern Horticulture,2015,39(06):86-89.[doi:10.11937/bfyy.201506024]
LED光源不同光质比对白掌组培苗生长的影响
- Title:
- Effect of Different Light Quality Ratios of LED on Growth of Spathiphyllum in vitro
- Keywords:
- Spathiphyllum; plantlets in vitro; LED; light quality ratios
- 文献标志码:
- A
- 摘要:
- 以白掌试管苗为试验材料，采用LED光源的蓝光（100%B）、80%R+20%B、70%R+30%B、50%R+50%B、红光（100%R）5种不同光质配比，以普通荧光灯（PGFL）作为对照，探讨不同光质比例对白掌组培苗生长的影响。结果表明：在80%R+20%B处理下，白掌组培苗的叶长、叶幅、根数、最大根长、根系活力、整株鲜重、地上鲜重和地下鲜重都达到最大值。因此，适合白掌组培苗生长的LED红蓝光的最佳比例为8∶2。
- Abstract:
- Taking Spathiphyllum in vitro as material,the aim of this research was to evaluate the effect of different 1ight quality ratios of LED on the growth of Spathiphyllum in vitro with different light quality ratios (100%B,80%R+20%B,70%R+30%B,50%R+50%B,100%R),as well as PGFL(CK）.The results showed that,in the light intensity of the red/blue ratio of 8∶2,the leaf length,blade width,root number，the biggest root length，root vigour，fresh weight and so on all reached the maximum.It’s found that the LED of the red/blue ratio of 8∶2 was the best way to cultivate in Spathiphyllum in vitro.
参考文献/References:
[1]许桂芳,董诚明,周吉源，等.不同光质对华黄芪愈伤组织诱导、增殖及器官分化的效应[J].华中师范大学学报(自然科学版),1994,28(4):533-536. [2]苏达,王德苗.大功率LED散热封装技术研究的新进展[J].电力电子技术,2007,41(10):13-15. [3]张燕.园林花卉学[M].北京:中国林业出版社,2003:294-295. [4]张志良,翟伟菁.植物生理学实验指导[M].北京:高等教育出版社,2004:15-20. [5]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:25-40. [6]刘文科,杨其长.LED植物光质生物学与植物工厂发展[J].科技导报,2014,32(10)：25-28. [7]郭云香,项丽敏,黄宏,等.LED在生物产业中的应用研究[J].现代农业科技,2012（10）:26-32. [8]Nhut D T,Takamura T,Watanabe H,et al.Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting dindes(LEDs)[J].Plant Cell,2010，73:43-52. [9]诸葛强,关亚丽,施季森,等.组培新技术及其在桉树快繁中的应用[J].林业科技开发，2003,17(6):37-38. [10]车生泉,盛月英,秦文英.光质对小苍兰茎尖试管培养的影响[J].园艺学报,1997,24(3):269-273. [11]Read P E，Economou A.Supplemental lighting in the propagation of deciduous azaleas[J].Proceedings of International Plant Propagators’ Society，1982，32：639-645. [12]岳岚,张玉芳,何松林,等.植物组织培养新技术的应用现状与发展趋势[J].现代园林,2008(3):48-51. [13]张婕,高亦珂,何琦,等.发光二极管(LED)在菊花组织培养中的应用研究[C]//中国观赏园艺研究进展2008—中国园艺学会观赏园艺专业委员会2008年学术年会论文集.北京:中国林业出版社,2008:296-299. [14]Tanaka M,Takamura T,Watanabe H,et al.In vitro growth of Cymidium plantlets cultured under superbright red and blue light-emitting diodes(LEDs)[J].Journal of Horticultural Science,1998,73(1):39-44. [15]刘晓英,焦学磊,徐志刚,等.红蓝LED光对叶用莴苣生长、营养品质和硝态氮含量的影响[J].南京农业大学学报,2013,36(5):139-143. [16]廖建良,刘欢欢,贺握权,等.LED补光对金银花栽培影响研究[J].惠州学院学报(自然科学版),2013(12):54-58. [17]苏娜娜,邬奇,崔瑾.LED光质补光对黄瓜幼苗生长和光合特性的影响[J].中国蔬菜,2012(24):48-54. [18]王忠.植物生理学[M].北京:中国农业出版社,2010:121-150. [19]杨艳琼,王荔,陈疏影,等.不同光照强度对灯盏花无糖组培苗生长发育的影响[J].云南农业大学学报,2007,22(3):323-326. [20]邸秀茹,焦学磊,崔瑾,等.新型光源LED辐射的不同光质配比光对菊花组培苗生长的影响[J].植物生物学通讯,2008(8):661-664. [21]李合生.现代植物生理学[M].北京:高等教育出版社,2012:70-94. [22]Saebo A，Krekling T，Appelgren M.Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro[J].Plant Cell Tissue and Organ Culture，2011,41(2):177-185. [23]郭子霞,尚文倩,闫新房,等.光照强度对白掌试管苗生长的影响[J].江西农业大学学报,2011(6):1131-1138. [24]王政,李高燕,何松林,等.不同比例光质冷阴极荧光灯(CCFL)培养后非洲菊试管苗移栽生长情况的研究[J].河南农业科学,2010(11):85-89.
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备注/Memo
第一作者简介：陈星星（1983-），女，河南南阳人，硕士，讲师，研究方向为园林植物。E-mail:abing2745@sina.com. 责任作者：何松林（1965-），男，博士，教授，研究方向为园林植物生物技术。E-mail:hsl213@163.com. 基金项目：河南省重大科技攻关资助项目（132102110184）。