[1]徐建中,陈金芳,杨玉芳,等.辣椒离体培养及再生体系的研究[J].北方园艺,2015,39(05):100-103.[doi:10.11937/bfyy.201505031]
　XU Jian-zhong,CHEN Jin-fang,YANG Yu-fang,et al.Study on in vitro Culture and Regeneration System of Pepper（Capsicum annuum L.）[J].Northern Horticulture,2015,39(05):100-103.[doi:10.11937/bfyy.201505031]
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辣椒离体培养及再生体系的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年05 页码: 100-103 栏目: 出版日期: 2015-03-14
Title:
Study on in vitro Culture and Regeneration System of Pepper（Capsicum annuum L.）
作者:
徐建中1; 2; 陈金芳1; 2; 杨玉芳2; 宋伟光2; 田 洪2
（1.河北省天然色素工程技术研究中心，河北 邯郸 057250；2.晨光生物科技集团股份有限公司，河北 邯郸 057250）
Author(s):
XU Jian-zhong1; 2; CHEN Jin-fang1; 2; YANG Yu-fang2; SONG Wei-guang2; TIAN Hong2
(1.Engineering Research Center of Natural Pigment in Hebei Province，Handan，Hebei 057250；2.Chenguang Biotech Group Co.Ltd.，Handan，Hebei 057250)
关键词:
辣椒; 植株再生体系; 离体培养; 带柄子叶
Keywords:
pepper; plant regeneration; in vitro culture; cotyledon with petiole
DOI:
10.11937/bfyy.201505031
文献标志码:
A
摘要:
以“韩国甜椒”为试材，研究外植体种类、苗龄、基本培养基种类、激素组合等多种因素对辣椒组织再生的影响。结果表明：带柄子叶再生能力较子叶和下胚轴切段强，14 d苗龄幼苗的外植体芽分化率较高，最佳芽分化培养基为MB+4.5 mg/L BA+0.5 mg/L IAA+4 mg/L AgNO3+200 mg/L Cef，最佳芽伸长培养基为MS+3 mg/L BA+0.3 mg/L IAA+2 mg/L GA3+4 mg/L AgNO3+200 mg/L Cef，生根培养基先用1/2MS+0.4 mg/L NAA+4 mg/L AgNO3，继代用1/2MS+0.4 mg/L NAA+0.2% 活性炭。
Abstract:
The effect of different explants，age of seedling，different basic mediums and combinations of hormones on plant regeneration were investigated by using the variety of pepper（Capsicum annuum L.）‘South Korea pepper’ as experiment material.The results showed that cotyledon with petiole taken from 14-day-old seedlings was much easier to induce regeneration than cotyledon and hypocotyl.The optimal medium for the differentiation of pepper buds was MB+4.5 mg/L 6-BA+0.5 mg/L IAA+4 mg/L AgNO3+200 mg/L Cef，the medium for the bud elongation was MS+3 mg/L 6-BA+0.3 mg/L IAA+2 mg/L GA3+4 mg/L AgNO3+200 mg/L Cef and the medium for rooting culture was 1/2MS+0.4 mg/L NAA+4 mg/L AgNO3 and 1/2MS+0.4 mg/L NAA+0.2% activated carbon as subculture medium.

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备注/Memo

第一作者简介：徐建中（1983-），男，硕士，工程师，研究方向为蔬菜生物技术与遗传育种。E-mail:xujianzhong1983@163.com.收稿日期：2014-11-10

更新日期/Last Update: 2015-06-24