GUO Ya-hua,XIE Li-bo,WANG Xue,et al.The RAPD Detection of Capsicum annuum L. After Ground Simulation[J].Northern Horticulture,2014,38(05):95-97.
辣椒经地面模拟诱变后的RAPD鉴定
- Title:
- The RAPD Detection of Capsicum annuum L. After Ground Simulation
- 文章编号:
- 1001-0009（2014）05-0095-03
- 分类号:
- S 641.3
- 文献标志码:
- A
- 摘要:
- 以地面模拟处理辣椒干种子为试材，采用RAPD技术对辣椒染色体进行多态性检测，研究了不同剂量的物理诱变处理对辣椒产生的影响。结果表明：采用Li离子照射可以诱导辣椒发生遗传位点的改变，但是不同剂量的效应不同；采用物理进行诱变可以诱导多个性状发生改变，该方法可以产生复杂的遗传变异。
- Abstract:
- Taking the seeds of Capsicum annuum L. by ground simulation from Heilongjiang Academy of Agricultural Sciences as materials,using RAPD (Random Amplified Polymorphic DNA) to detect polymorphism of pepper,the influence of different doze of physical simulation on pepper were studied. The results showed that the genetic site could be changed after Li treatment,however,different doze had different effects;many characteristics could be changed by physical simulation,accordingly,this method could produce a complex genetic variation.
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备注/Memo
第一作者简介：郭亚华（1953-），女，黑龙江哈尔滨人，研究员，现主要从事植物空间诱变育种和生物技术工作。E-mail: guoyahua@sina.com.