YE Zhen-mei.Study on Optimization of Extracting Technology of Polysaccharides from Poria coccus by Response Surface Methodology and Its Immune Activity[J].Northern Horticulture,2013,37(17):135-138.
茯苓多糖的响应面提取条件优化及体外免疫活性研究
- Title:
- Study on Optimization of Extracting Technology of Polysaccharides from Poria coccus by Response Surface Methodology and Its Immune Activity
- 文章编号:
- 1001-0009(2013)17-0135-04
- 分类号:
- S 567.3+2
- 文献标志码:
- A
- 摘要:
- 以茯苓为原料，研究了茯苓多糖的响应面提取条件优化及体外免疫活性。结果表明：茯苓多糖提取最佳工艺条件为微波功率471.3 W，微波提取时间8.5 min，乙醇浓度82.3%，液料比为19.9，在该条件下，茯苓多糖的提取率为2.96%。茯苓多糖具有较好的刺激淋巴细胞增殖的能力，随着茯苓粗多糖浓度的增加，其刺激淋巴细胞增殖的能力也逐渐增强，当茯苓粗多糖浓度超过100 μg/mL时，其刺激淋巴细胞增殖的能力反而下降。
- Abstract:
- Taking Poria coccus as material,the extraction technology of polysaccharides from it was optimized by response surface methodology and its immune activity was studied.The results showed that the optimum extraction conditions of polysaccharides from Poria coccus was microwave power 471.3 W,microwave extraction time 8.5 min,ethanol concentration 82.3%,solution and material ratio 19.9,under these conditions,the polysaccharides yield could reach 2.96%.The polysaccharides from Poria coccus had proliferative effect on leukomonocyte.The proliferative effect strengthened gradually with the concentration of polysaccharides from Poria coccus increased.When its concentration exceeded 100 μg/mL,the proliferative effect decreased.
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备注/Memo
作者简介：叶振梅（1967-），女，硕士，高级讲师，研究方向为妇产科与人体免疫学。
收稿日期：2013-04-10