LIN Chao,MA Xin-ye,LIU Feng,et al.Study on Callus Induction from Leaf Explants of Aquilaria sinensis[J].Northern Horticulture,2013,37(08):97-100.
白木香叶片外植体愈伤组织诱导研究
- Title:
- Study on Callus Induction from Leaf Explants of Aquilaria sinensis
- 文章编号:
- 1001-0009（2013）08-0097-04
- Keywords:
- Aquilaria sinensis; callus; induction
- 分类号:
- S 792.119
- 文献标志码:
- A
- 摘要:
- 以白木香叶片为试材，研究了叶龄、外植体大小、外植体接种方式、光照和激素浓度及组合等因素和水平对愈伤组织诱导的影响。结果表明：选择叶龄为12 d的叶片剪成0.5 cm2大小，以正面向上的方式接种在MS+2,4-D 1.0 mg/L+ZT 1.0 mg/L培养基中，黑暗培养，愈伤组织诱导率达100%。
- Abstract:
- Taking the leaves of Aquilaria sinensis as experimental materials,the leaflet age，explants size，explants inoculation，lighting，concentration and combination of hormone on callus induction were studied.The results showed that the leaflet age 12 d blades cut into 0.5 cm2 segments，face up vaccination on MS medium add with 1.0 mg/L 2,4-D and 1.0 mg/L ZT，dark culture，callus induction rate could reach 100%.
参考文献/References:
[1]国家药典委员会．中华人民共和国药典·一部[M].北京:中国医药科技出版社,2010:172-173. [2]国家环境保护局,中国科学院植物研究所.中国珍稀濒危保护植物名录[M].1册.上海:上海科技教育出版社,1987:94. [3]《国家重点保护野生植物名录(第一批)》1999年8月4日国务院批准[J].环境教育,2008(7)：84. [4]兰芹英,方春妍,何惠英,等.土沉香成熟胚的组织培养及植株再生[J].广西农业生物科学,2001,20(3):231-232. [5]张翘,郑希龙,潘超美.不同培养体系对白木香成熟胚丛芽诱导的影响研究[J].时珍国医国药,2009,20(10):2566-2567. [6]杜勤,王振华,刘淑芬,等.白木香组织培养的初步研究[J].中国中药杂志,2001,26(10):679-680. [7]叶勤法,戚树源,林立东.土沉香愈伤组织培养及植株再生(简报)[J].热带亚热带植物学报,1998,6(2):172-176. [8]张彦妮.影响植物组织培养成功的因素[J].北方园艺,2006(3):132-133. [9]周春娜,郑辉,宋立功,等.外植体及培养基对欧洲白桦器官再生的影响[J].河北林果研究,2006,21(1):1-4. [10]李培英,孙宗玖,张博.激素、培养基、添加物对苜蓿子叶再生体系建立影响[J].新疆农业科学,2009,46(4):824-828. [11]丁霞,陈晓阳,李云,等.胡杨叶片不定芽再生体系的研究[J].北京林业大学学报,2003,25(2):28-31. [12]唐建军,陈欣,志水胜好.培养条件对杜仲愈伤组织形成及次生代谢过程的影响[J].浙江大学学报,2002,36(2):193-198. [13]王彦文,黄艳红,路国兵,等.桑树叶片愈伤组织的诱导及不定芽分化影响因素的研究[J].蚕业科学,2006,32(2):157-160. [14]薛建平,王汝毅,张爱民,等.地黄叶片器官形态建成的研究[J].中国中药杂志,2003,28(1):21-24. [15]王跃华,张阔军,张丽君,等.川贝母叶高效诱导愈伤组织体系的研究[J].安徽农业科学,2011,39(3):1374-1375.
相似文献/References:
[1]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(08):108.
[2]闫海霞,黄昌艳,何荆洲,等.观赏凤梨组织培养的影响因素分析[J].北方园艺,2013,37(22):196.
YAN Hai-xia,HUANG Chang-yan,HE Jing-zhou,et al.Analysis of the Influence Factors in Tissue Culture of Ornamental Bromeliads[J].Northern Horticulture,2013,37(08):196.
[3]王 婷,智永祺,周 坚.长筒石蒜叶片薄层培养诱导愈伤组织发生的研究[J].北方园艺,2014,38(01):91.
WANG Ting,ZHI Yong-qi,ZHOU Jian.Study on Callus Induction of the Leaf Blades of Lycoris longitubaUsing Thin Cell Layer Culture[J].Northern Horticulture,2014,38(08):91.
[4]焦战战,张东向,赵静,等.黄芩愈伤组织中黄芩苷与过氧化物酶活性相关性分析[J].北方园艺,2013,37(13):108.
JIAO Zhan-zhan,ZHANG Dong-xiang,ZHAO Jing,et al.Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi[J].Northern Horticulture,2013,37(08):108.
[5]罗君琴,徐建国,王平.不同培养条件对柑桔胚性愈伤组织离体诱导的影响[J].北方园艺,2013,37(13):125.
LUO Jun-qin,XU Jian-guo,WANG Ping.Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus[J].Northern Horticulture,2013,37(08):125.
[6]马少梅.北海道黄杨再生体系建立研究进展[J].北方园艺,2013,37(13):208.
MA Shao-mei.Research Advances on the Establishment of Regeneration System of Euonymus japonicus[J].Northern Horticulture,2013,37(08):208.
[7]于 娜,董宽虎.白羊草成熟种子组织培养再生体系研究[J].北方园艺,2014,38(02):104.
YU Na,DONG Kuan-hu.Research on Tissue Culture of Regeneration System of the Mature Seeds of Bothriochloa ischaemum [J].Northern Horticulture,2014,38(08):104.
[8]张丽霞,王毅彰.植物生长调节剂对花籽山药愈伤组织诱导形成的影响[J].北方园艺,2013,37(14):115.
ZHANG Li-xiaWANG Yi-zhang.Effect of Plant Growth Substances on Callus Induction of Seed Yam Forming[J].Northern Horticulture,2013,37(08):115.
[9]李金英,赵春莉,张馥茜,等.黑穗醋栗愈伤组织诱导研究[J].北方园艺,2013,37(17):87.
LI Jin-ying,ZHAO Chun-li,ZHANG Fu-qian,et al.Study on the Callus Induction of Blackcurrant[J].Northern Horticulture,2013,37(08):87.
[10]于艳萍,韩善峰,赵登超,等.嫩枝扦插不同处理对“香玲”核桃组织再生能力的影响[J].北方园艺,2013,37(17):91.
YU Yan-ping,HAN Shan-feng,ZHAO Deng-chao,et al.Effect of Different Softwood Cutting Treatments on the Tissue Regenerating Ability of Juglans regia ’Xiangling’[J].Northern Horticulture,2013,37(08):91.
备注/Memo
第一作者简介：林超（1987-），男，硕士，现主要从事植物组织培养和育种方面的研究工作。E-mail：zhelinlengshan@163.com.