SHI Cai-juan,FAN Gao-tao,ZHU Meng-li,et al.Culture of Strong Regenerated Seedling of Dendrobium candidum Wall ex Lindl.[J].Northern Horticulture,2013,37(06):90-93.
黑节草再生植株壮苗培养条件的优化
- Title:
- Culture of Strong Regenerated Seedling of Dendrobium candidum Wall ex Lindl.
- 文章编号:
- 1001-0009(2013)06-0090-04
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以黑节草种子为外植体，研究比较了基本培养基、蔗糖、激素及激素组合对黑节草试管苗生长的影响。结果表明：以1/2MS大量元素、MS全量微量元素及有机物为基本培养基，附加2%蔗糖、1.0 mg/L NAA和1.0 mg/L IAA，组成壮苗培养基，能有效地诱导再生植株的新根萌发并能促进茎和根的发育。
- Abstract:
- Taking the seeds of Dendrobium candidum Wall ex Lindl.as material，the effect of media with sucrose，hormones and hormone combinations of NAA (Naphthaleneacetic acid)，IAA (3-indoleacetic acid) and IBA (3-indolebutyric acid) on growth of plantlets were studied.The results showed that the new roots of regenerated plantlets could be efficiently induced，and the development of stems and roots could be promoted on the basal medium with half of macronutrients and all the other micronutrients and organic nutrients of MS (Murashige and Skoog) medium，supplemented with 1.0 mg/L NAA，1.0 mg/L IAA and 2% sucrose.
参考文献/References:
[1]
丁小余
,
徐珞珊
,
王峥涛
,
等
.
铁皮石斛群居差异的研究
[J].
中草药
,2001,32(9):828-831.
[2]邵华,张玲琪,李俊梅,等.铁皮石斛研究进展[J].中草药,2004,35(1):109-112. [3]Nayak N R，Rath S P，Patnaik S.In vitropropogation of three epiphytic orchids，Cymbidium aloifolium(L.) Sw.，Dendrobium aphyllum(Robx.) Fisch.and?Dendrobium moschatum(Buch-Ham) Sw.through thidiazuron-induced high frequency shoot proliferation[J].Scientia Horticulturae,1997,71:243-250. [4]Nayak N R，Sahoo S，Patnaik S,et al.Establishment of thin cross section (TCS) culture method for rapid micropropagation of?Cymbidium aloifolium(L.) Sw.and Dendrobium nobile?Lindl.(Orchidaceae)[J].Scientia Horticulturae,2002,94:107-116. [5]王进红,张雪梅,付开聪.黑节草茎段直接诱导丛生芽[J].时珍国医国药,2000,11(11):1052. [6]张泉锋,毛碧增.铁皮石斛培养的产业化研究[J].中草药,2004,35(4):438-440. [7]刘用生,李友勇.植物组织培养中活性炭的使用[J].植物生理学通讯,1994,30(3):214-217.
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备注/Memo
第一作者简介：石彩娟（1987-），女，河北邯郸人，硕士，现主要从事药用植物资源与分子生物学的研究工作。E-mail:caijuanshi@163.com.