[1]施柳,王雅琼,李云龙,等.不同基因型大白菜小孢子胚状体诱导及植株再生[J].北方园艺,2014,38(06):101-104.
　SHI Liu,WANG Ya-qiong,LI Yun-long,et al.Microspore Embryogenesis and Plantlet Formation of Different Genotypes of Brassica rapa ssp.pekinensis[J].Northern Horticulture,2014,38(06):101-104.
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不同基因型大白菜小孢子胚状体诱导及植株再生

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年06期 页码: 101-104 栏目: 出版日期: 2014-03-30
Title:
Microspore Embryogenesis and Plantlet Formation of Different Genotypes of Brassica rapa ssp.pekinensis
文章编号:
1001-0009(2014)06-0101-04
作者:
施柳1; 2; 王雅琼1; 2; 李云龙1; 2; 朱祝军1; 2; 郑伟尉1; 2; 臧运祥1; 2
1.浙江农林大学 农业与食品科学学院，浙江省农产品品质改良技术研究重点实验室，浙江 临安 311300;
2.浙江农林大学 生物种业研究中心，浙江 临安 311300
Author(s):
SHI Liu1; 2; WANG Ya-qiong1; 2; LI Yun-long1; 2; ZHU Zhu-jun1; 2; ZHENG Wei-wei1; 2; ZANG Yun-xiang1; 2
1.The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,Research Center of Bio-Breeding Industry,College of Agriculture and Food Science,Lin’ an,Zhejiang 311300；
2.Zhejiang Agricultural and Forestry University,Lin’ an,Zhejiang 311300
关键词:
大白菜; 小孢子培养; 胚状体; 再生植株
Keywords:
Brassica rapa ssp.pekinensis; microspore culture; embryoid; plantlet
分类号:
S 634.1
文献标志码:
A
摘要:
以9个大白菜品种的花蕾为试材，研究了基因型、6-BA以及不同固体培养基对大白菜小孢子胚发生的影响,以期为其它十字花科蔬菜小孢子培养体系的建立提供技术参考。结果表明：不同基因型大白菜的小孢子胚诱导率具有明显差异，9个大白菜品种中有6个诱导出胚状体，‘北京特好吃火锅菜’出胚率最高，达到每蕾12.38胚；添加0.3 mg/L的6-BA可以促进小孢子胚状体的形成，以‘北京特好吃火锅菜’提升效果最显著；添加0.01 g/L活性炭的MS固体培养基比B5培养基更适于成熟胚状体发育成苗。
Abstract:
Taking flower buds of 9 Brassica rapa ssp.pekinensis cultivars as materials,the effect of genotypes,6-BA and different solid medium on microspore embryogenesis were studied.The results showed that the embryogenesis rate was significantly different among cultivars.Six cultivars were induced to form embryoids and the highest yield was produced in ‘Beijing Tehaochihuoguocai’ with 12.38 embryoids per bud.Medium supplemented with 0.3 mg/L 6-BA could promote the embryogenesis rate,especially for ‘Beijing Tehaochihuoguocai’.The solid MS medium supplemented with 0.01 g/L active carbon was better than B5 solid medium for plantlet formation from embryoids.

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备注/Memo

第一作者简介：施柳（1990-），女，上海人，硕士研究生，研究方向为蔬菜生物技术。E-mail：shiliu0719@126.com.

责任作者：臧运祥（1978-），男，副教授，现主要从事园艺植物生物技术与分子育种研究工作。E-mail：yxzang@zafu.edu.cn.
基金项目：国家自然科学基金资助项目（31000916）；浙江省重大科技资助项目(2010C12004)；浙江省本科院校中青年学科带头人学术攀登资助项目（pd2013230）；浙江农林大学人才启动资助项目（2009FR047）；浙江农林大学生物种业研究中心预研基金资助项目；浙江农林大学研究生科研创新基金资助项目(3122013240261)。

更新日期/Last Update: 2014-08-11