WU Yan-sheng.Study on the Induction Conditions of Polyploid Garlic Embryoid[J].Northern Horticulture,2013,37(11):110-112.
多倍体大蒜胚状体诱导条件研究初报
- Title:
- Study on the Induction Conditions of Polyploid Garlic Embryoid
- 文章编号:
- 1001-0009(2013)11-0110-03
- 分类号:
- S 335.1；S 633.4
- 文献标志码:
- A
- 摘要:
- 以大蒜根尖为试材，采用0.05%秋水仙素溶液诱导多倍体，研究了愈伤组织的诱导和培养方法，以及胚状体的诱导方法，以期为大蒜胚状体诱导提供依据。结果表明：在MS+2,4-D 2.0 mg/L+KT 0.5 mg/L培养基上，大蒜根尖可形成疏松型和致密型2种愈伤组织结构；将致密型愈伤组织培养在MS+KT3.0 mg/L+NAA 0.5 mg/L培养基上，将形成胚状体。
- Abstract:
- Taking the root tip of garlic as material,the induction and culturing methods of callus and induction methods of embryoid were investigated after treated with colchicine,in order to provide basis for enduction of garlic embryoid.The results indicated that on the medium MS+2,4-D 2.0 mg/L+KT 0.5 mg/L,the root tip of garlic could form soft and dense callus；on the medium MS+KT 3.0 mg/L+NAA 0.5 mg/L,embryoid could be gotten from dense callus.
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备注/Memo
作者简介：武延生（1977-），男，河北临西人，硕士，讲师，现主要从事作物育种及植物信号转导等研究工作。
基金项目：邢台市科技计划资助项目（2011ZC017）。
收稿日期：2013-01-16