WANG Chen-yu,LIU Xin,ZHANG Jing,et al.Study on Extraction of Water solubility Polysaccharide from Gentiana scabra Bunge[J].Northern Horticulture,2013,37(10):155-157.
水溶性龙胆多糖的提取工艺研究
- Title:
- Study on Extraction of Water solubility Polysaccharide from Gentiana scabra Bunge
- 文章编号:
- 1001-0009(2013)10-0155-03
- Keywords:
- polysaccharides; optimization; Gentiana scabra Bunge; water solubility
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 以龙胆为试材，采用水提醇沉法，在单因素试验基础上，进行L9(33)正交实验优化设计，研究了水溶性龙胆多糖的最佳提取工艺条件，以期考查提取温度、提取时间、提取次数、液料比等因素对多糖提取率的影响。结果表明：提取温度100℃，料液比1∶25，提取时间3.0 h，提取次数2次,此时水溶性龙胆多糖的提取率最高，可达(14.0±0.37)%。
- Abstract:
- Taking Gentiana scabra Bunge as material,the method of water extraction and alcohol sink were used to extract water solubility polysaccharides from the Gentiana scabra Bunge.The influence of extraction temperature，extraction time，extraction number and ratio of water to raw material on the yield of Gentiana scabra Bunge polysaccharides (GSP) were investigated by L9(33) orthogonal test.The results showed that the extraction temperature was 100℃，ratio of water to raw material was 1∶25，extraction time was 3.0 h，and extraction times was two.Under the optimize conditions，the experimental yield of GSP could reach (14.0±0.37)%.
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备注/Memo
第一作者简介：王晨瑜(1986-)，女，山东滨州人，在读硕士，研究方向为生物化工。E-mail:dennistt@126.com.
责任作者：王战勇(1978-)，男，辽宁抚顺人，博士，副教授，研究方向为生物活性物质开发。E-mail:wangzy125@gmail.com.
基金项目：抚顺市科学技术研究资助项目(FSKJHT201135)。
收稿日期：2013-01-25