[1]罗 群,张 宏.菜籽蛋白提取工艺的优化及其对DPPH自由基的清除作用[J].北方园艺,2014,38(14):136-139.
　LUO Qun,ZHANG Hong.Optimization of Protein Extraction Process and Scavenging Function of DPPH Radical From Rapeseed Meal[J].Northern Horticulture,2014,38(14):136-139.
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菜籽蛋白提取工艺的优化及其对DPPH自由基的清除作用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年14期 页码: 136-139 栏目: 出版日期: 2014-07-30
Title:
Optimization of Protein Extraction Process and Scavenging Function of DPPH Radical From Rapeseed Meal
文章编号:
1001-0009(2014)14-0136-04
作者:
罗 群1; 张 宏2
1.成都师范学院 教育科学学院，四川 成都 611130；
2.四川师范大学 生命科学学院，四川 成都 610066
Author(s):
LUO Qun1; ZHANG Hong2
1.College of Education Science,Chengdu Normal College,Chengdu,Sichuan 611130;
2.College of Life Science,Sichuan Normal University,Chengdu,Sichuan 610066
关键词:
菜籽粕; 可溶性蛋白质; 优化; 自由基清除
Keywords:
rapeseed meal; soluble protein; optimization; free radical scavenging
分类号:
S 794.4
文献标志码:
A
摘要:
以高温热榨菜籽粕为试材，研究了碱提酸沉法提取菜籽蛋白的最佳工艺。结果表明：在pH值为11.5，提取温度55℃，料液比1∶15 g/mL，超声功率80 W，提取时间100 min，提取次数为3次的条件下，菜籽可溶性蛋白的最佳提取率为28.21%，菜籽产品蛋白得率为23.70%。由该产品蛋白分离制备的清蛋白和谷蛋白对DPPH自由基的清除率分别为31.09%和29.08%，具有较强的自由基清除作用。
Abstract:
Taking hot-pressed rapeseed meal as material,the optimum protein extraction process by alkali extraction and acid precipitation was studied.The results showed that the optimal conditions of extraction were follows:pH 11.5,temperature 55℃,ratio of material to liquid 1∶15 g/mL,ultrasonic power 80 W,extraction time 100 min,3 times of extraction.Under these conditions,the extraction yield of soluble protein from rapeseed meal was 28.21%,the rapeseed protein yield of end product was 23.70%.The albumin and globulins by isolation of rapeseed protein had powerful scavenging efficiency to DPPH radical,the clearance rate were 31.09% and 29.08% respectively.

参考文献/References:

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[2] Zhang S B,Wang Z, Xu S Y.Antioxidant and antithrombotic activities of rapeseed peptides[J]. Joumal of the American Oil Chemists’ Society,2008,85(6)：521-527.
[3]钮淡星，黄凤洪，倪光远，等．菜籽粕的饲用现状和饲用改良技术发展趋势[J]．中国油脂,2009, 34(5)：4-7．
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备注/Memo

第一作者简介：罗群（1979-），女，硕士，讲师，现主要从事生物学及预防医学等研究工作。E-mail:372384664@qq.com.

责任作者：张宏（1965-），女，博士，教授，硕士生导师，现主要从事天然产物化学等研究工作。E-mail:401923129@qq.com.
基金项目：四川省教育厅基金资助项目（2006B035）。
收稿日期：2014-03-13

更新日期/Last Update: 2014-08-07