ZHANG Cun-zhi,WEI Tian-jun,WEI Peng,et al.Establish the Efficient System of Subculture Proliferation About Zizyphus jujube Mill cv.Lingwuchangzao[J].Northern Horticulture,2012,36(16):95-97.
灵武长枣高效继代增殖体系的建立
- Title:
- Establish the Efficient System of Subculture Proliferation About Zizyphus jujube Mill cv.Lingwuchangzao
- 文章编号:
- 1001-0009（2012）16-0095-03
- Keywords:
- jujube; axillary buds; subculture proliferation
- 分类号:
- S 665.103.6
- 文献标志码:
- A
- 摘要:
- 对灵武长枣茎段离体培养、高效继代增殖体系进行了研究。结果表明：以灵武长枣当年萌发幼嫩枣头为外植体，诱导腋芽萌发，细胞分裂素/生长素的比值为5倍时，腋芽萌发率最高；将萌发的嫩梢进行继代增殖培养，继代增殖茎段不同的放置方式、茎段不同位置、培养基的相态对增殖倍数有影响。
- Abstract:
- The efficient subculture proliferation system and stem section in vitro culture Zizyphus jujube Mill cv.Lingwuchangzao were studied.The results showed that with the germination young jujube head of lingwuchangzao as explants,induced axillary buds,when cytokinin/auxin ratio was 5 times,the rate of axillary buds was the highest.Germination of young shoots was subcultured,the proliferation of multiples was different about stem segments of different placement,the different position of the stem and solid-liquid of the medium.
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备注/Memo
第一作者简介：张存智（1973-），女，硕士，副教授，现主要从事果树生物技术育种方面的研究工作。E-mail:zhangcunzhi72@126.com.