[1]孙骏威,方晓峰,陈珍.不同植物生长调节剂对黄秋葵组织培养的影响[J].北方园艺,2012,36(07):139-141.
　SUN Jun-wei,FANG Xiao-feng,CHEN Zhen.Effects of Plant Growth Regulator on Tissue Culture of Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.[J].Northern Horticulture,2012,36(07):139-141.
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不同植物生长调节剂对黄秋葵组织培养的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年07期 页码: 139-141 栏目: 出版日期: 2012-07-16
Title:
Effects of Plant Growth Regulator on Tissue Culture of Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.
文章编号:
1001-0009（2012）07-0139-03
作者:
孙骏威1; 方晓峰1; 陈珍2
1.中国计量学院 生命科学学院，浙江 杭州 310018；
2.台州学院 生命科学学院，浙江 临海 317000
Author(s):
SUN Jun-wei1; FANG Xiao-feng1; CHEN Zhen2
1.College of Life Sciences，China Jiliang University，Hangzhou,Zhejiang 310018；2.School of Life Sciences，Taizhou University，Linhai，Zhejiang 317000
关键词:
黄秋葵; 植物生长调节剂; 茎段; 丛生芽诱导; 生根
Keywords:
Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.]; plant growth regulator; stem segments; induction of clumpy buds; rooting
分类号:
S 649
文献标志码:
A
摘要:
以黄秋葵无菌实生苗的带一叶一芽的茎段为外植体，研究了不同植物生长调节剂处理对丛生芽诱导和不定芽生根的影响。结果表明：当外植体接种于1/2MS 基本培养基中，一定的浓度范围内，IAA 浓度的增加显著促进了黄秋葵茎段的分化率和增殖率，而 6-BA 的存在提高了IAA的这种促进作用，但并不明显，最优组合为IAA 1.0 mg/L+6-BA 2.0 mg/L。待丛生芽生长到1 cm以上，切出单个不定芽，接入添加不同NAA浓度的1/4MS基本培养基中，发现生根率和生长状况在NAA 0.1 mg/L时表现最好。
Abstract:
The effects of different plant growth regulator treatments on induction of clumpy buds and rooting in Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.] plants were studied，the stem segments with one leaf and one axillary bud of seedlings derived from sterile germination were used as explants.The results showed that when the explants were inoculated on 1/2MS basic medium，in a certain concentration range，the increase of IAA concentrations significantly promoted the ratio of differentiation and proliferation，and the presence of 6-BA could increase the role of IAA treatments，but not obviously，so the optimal combination was IAA 1.0 mg/L+6-BA 2.0 mg/L.After the buds grew to more than 1 cm，the single buds were cut out to inoculate on 1/4MS basic medium adding different NAA concentrations，and the optimal rooting rate and growth performance was found in the treatment of NAA 0.1 mg/L.

参考文献/References:

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备注/Memo

第一作者简介：孙骏威(1978-)，男，硕士，讲师，现主要从事植物生理学研究工作。E-mail：juville@cjlu.edu.cn。基金项目：台州科技局资助项目(071KY07)。收稿日期：2012-01-10

更新日期/Last Update: 2014-09-03