QI Cui-ying,LI Xu-xia,WANG Ning,et al.Study of Tissue Culture Technique of Salvia miltiorrhiza f.alba[J].Northern Horticulture,2012,36(06):108-110.
白花丹参组织培养技术的研究
- Title:
- Study of Tissue Culture Technique of Salvia miltiorrhiza f.alba
- 文章编号:
- 1001-0009（2012）06-0108-03
- Keywords:
- Salvia miltiorrhiza f.alba; callus; vitrification preventing; root
- 分类号:
- S 567.5+3
- 文献标志码:
- A
- 摘要:
- 以白花丹参叶为外植体，研究了外植体种类、激素比例、琼脂浓度、活性炭等培养条件对组培快繁的影响。结果表明：培养基MS+6-BA 2.0 mg/L+NAA 1.0 mg/L+琼脂7%适宜2种愈伤组织诱导；培养基 MS+KT 1.5 mg/L+NAA 0.5 mg/L+琼脂8%+活性炭0.3%能有效预防玻璃化苗，适宜健壮芽苗增殖；培养基1/2MS+IBA 0.2 mg/L+琼脂8%（或同时添加0.3%活性炭）有利于试管苗根系的生长，生根率达到100%。试管苗移栽成活率达到95%。
- Abstract:
- Leaves of Salvia miltiorrhiza.f.alba were used as explant,the effects of the explants variations,ratio of steroids,concentration of agar and active carbon on tissue culture and propagation were studied.The results indicated that the media of MS+6-BA 2.0 mg/L+NAA 1.0 mg/L+agar 7% fit the inducing of two types of calli,the MS+KT 1.5 mg/L+NAA 0.5 mg/L+agar 8%+active carbon medium was most suitable for decreasing vitrification of plantlets and fit to grow up strong seedlings,the 1/2MS+IBA 0.2 mg/L medium(or 0.3% active carbon simultaneously) was better for root generation with rooting rate of 100%.The survival rate of transplantation of test-tube seedling could reach a high level at 95%.
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备注/Memo
第一作者简介：亓翠英（1976-），女，山东莱芜人，硕士，讲师，研究方向为植物分子细胞遗传学。E-mail:qcuiying@163.com。