[1]王瑞雪,郭杰,郝楠,等.胡萝卜14-3-3基因家族的鉴定与分析[J].北方园艺,2024,(6):17-24.[doi:10.11937/bfyy.20233424]
　WANG Ruixue,GUO Jie,HAO Nan,et al.Identification and Analysis of 14-3-3 Gene Family in Daucus carota[J].Northern Horticulture,2024,(6):17-24.[doi:10.11937/bfyy.20233424]
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胡萝卜14-3-3基因家族的鉴定与分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年6 页码: 17-24 栏目: 出版日期: 2024-03-31
Title:
Identification and Analysis of 14-3-3 Gene Family in Daucus carota
文章编号:
1001-0009(2024)06-0017-08
作者:
王瑞雪; 郭杰; 郝楠; 朱丽君
（兰州职业技术学院，甘肃 兰州 730007）
Author(s):
WANG Ruixue; GUO Jie; HAO Nan; ZHU Lijun
(Lanzhou Vocational Technical College，Lanzhou，Gansu 730070)
关键词:
胡萝卜; 14-3-3基因家族; 系统进化分析
Keywords:
carrot; 14-3-3 gene family; phylogeny analysis
分类号:
S 631.2
DOI:
10.11937/bfyy.20233424
文献标志码:
A
摘要:
以胡萝卜为试材，采用分子生物学和生物信息学方法对胡萝卜14-3-3基因家族各成员进行鉴定，研究分析其理化特性、基因结构、保守结构域、复制事件、系统进化及顺式作用元件，以期为进一步研究胡萝卜14-3-3蛋白的功能提供参考依据。结果表明:胡萝卜中共鉴定到11个14-3-3基因，不均匀分布在6条染色体上，含有3~7个外显子，其中1对基因发生片段复制事件；编码的蛋白序列长度为236~264 aa，分子量为26.64~30.14 kD，等电点为4.65~5.33，具有7个Motif，全部为酸性非分泌型蛋白，定位在细胞核。系统进化分析显示，胡萝卜14-3-3蛋白分为ε类（3个）与非ε（8个）类2个亚族。此外，顺式作用元件分析结果表明，胡萝卜14-3-3基因含有大量激素与逆境胁迫相关元件。
Abstract:
Taking carrot as the test material,14-3-3 gene family in carrot were identified and their physicochemical properties，gene structure，conserved domains，replication events，phylogenetic evolution and cis-acting elements were analyzed,in order to provide reference for further understanding the function of 14-3-3 proteins in carrot.The results showed that a total of 11 14-3-3 genes were identified in carrot，unevenly distributed on six chromosomes，containing 3 to 7 exons，and one pair of 14-3-3 genes underwent segmental duplication.Carrot 14-3-3 proteins contained 236-264 amino acid residues，with molecular weight of 26.64-30.14 kD，isoelectric point of 5.84-9.14，and 7 conserved motifs.All 14-3-3 proteins of carrot were acidic and non-secreted proteins and located in the nucleus.Phylogenetic analysis indicated that the 14-3-3 proteins of carrot were divided into ε-type (3 members) and non ε-type (8 members).In addition，cis-acting element analysis showed that carrot 14-3-3 genes contained a large number of hormone-and stress-related elements.

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备注/Memo

第一作者简介：王瑞雪（1985-），女，硕士，讲师，现主要从事植物系统与进化等研究工作。E-mail：369906560@qq.com.基金项目：甘肃省高等学校创新基金资助项目（2022B-437）。收稿日期：2023-10-09

更新日期/Last Update: 2024-04-11