ZHU Qingsong,ZHAO Haiying,LIU Songhu.Induction and Preliminary Identification of Pinellia ternata (Thumb.) Breit Polyploids by Colchicine Immersion[J].Northern Horticulture,2025,(9):18-24.[doi:10.11937/bfyy.20243715]
秋水仙素浸泡法对旱半夏多倍体的诱导及初步鉴定
- Title:
- Induction and Preliminary Identification of Pinellia ternata (Thumb.) Breit Polyploids by Colchicine Immersion
- 文章编号:
- 1001-0009(2025)09-0018-07
- Keywords:
- Pinellia ternata(Thumb.) Breit; colchicine; polyploids; soaking method
- 分类号:
- S 567.239
- 文献标志码:
- A
- 摘要:
- 以旱半夏为试材，采用秋水仙素浸泡法，研究了不同的秋水仙素浓度和不同的处理时间对旱半夏多倍体成活率和诱导率的影响，以期为旱半夏多倍体的育种研究提供参考依据。结果表明：秋水仙素浸泡法获得旱半夏多倍体的最佳处理条件为25 ℃，0.06%秋水仙素处理4 d。显微观察发现，处理后的旱半夏多倍体出现不同水平的染色体加倍，气孔增大，气孔密度降低。与对照相比，旱半夏多倍体植株的叶片肥厚，植株粗壮，植株高度、叶柄直径、叶片大小、子代块茎直径和质量均显著增加。
- Abstract:
- Taking Pinellia ternata as experimental material,colchicine immersion method was adopted，the effects of different concentrations of colchicine and different treatment times on the survival and induction rates of polyploids of Pinellia ternata were studied，in order to provide reference for the breeding research of Pinellia ternata polyploids.The results showed that the optimal treatment conditions for obtaining Pinellia ternata polyploids by colchicine immersion method were at 25 ℃，with 0.06% colchicine treatment for 4 days.Microscopic observation revealed that the treated Pinellia ternata polyploids exhibited different levels of chromosome doubling，increased stomatal size，and decreased stomatal density.Compared with the control group，the leaves of the Pinellia ternata polyploid plants were thicker，the plants were sturdier，and the plant height，petiole diameter，leaf size，and the diameter and weight of the progeny tubers were significantly increased.
参考文献/References:
［1］姚军强.半夏的药理作用及其临床配伍运用［J］.中医研究,2013,26(2):3-5.［2］苏洪佳,陈国忠,谢君艳,等.浅析以半夏功效为发挥作用的基本框架［J］.中国中医基础医学杂志,2019,25(6):825-827.［3］黄际堂.《伤寒杂病论》中半夏的量效关系与应用特点研究［J］.内蒙古中医药,2017,36(16):91-92.［4］常琴,张明生,黄如意,等.半夏种质遗传多样性鉴定与产量决定因素分析［J］.北方园艺,2022(18):99-108.［5］李点点,初雷霞,余孟娟,等.基于ISSR不同繁殖方式半夏的遗传多样性研究［J］.北方园艺,2023(24):102-109.［6］王彩琴,杜娟,王一敏,等.甘肃半夏资源及研究现况［J］.西部中医药,2015,28(3):22-24.［7］张军民.旱半夏人工栽培技术的若干思考［J］.农业开发与装备,2016(11):143.［8］何韩军,杨跃生,吴鸿.药用植物多倍体的诱导及生物学意义［J］.中草药,2010,41(6):1000-1006.［9］郭巧萍.桃叶型半夏多倍体诱导研究［D］.杭州:浙江理工大学,2009.［10］GAO Z,ZHANG C C,LUO L N,et al.Physiological characteristics and transcriptome differences analysis of diploid and autotetraploid of Atractylodes lancea［J］.Journal of Nanjing Agricultural University,2020,43(6):1024-1032.［11］高继海,周罗静,尹彦棚,等.药用植物多倍体及对中药品种品质的影响［J］.中药与临床,2022,13(4):1-3,6.［12］刘茜,余磊磊,聂倩文,等.荆半夏多倍体新种质诱导研究［J］.长江大学学报(自然科学版),2018,15(10):28-33,5-6.［13］周慧文,冯斗,严华兵.秋水仙素离体诱导多倍体研究进展［J］.核农学报,2015,29(7):1307-1315.［14］欧巧明,岳春玲,厚毅清,等.基于悬浮细胞培养的半夏多倍体诱导研究:Ⅱ.单细胞水平COLO诱导及多倍体植株再生［J］.核农学报,2011,25(6):1148-1156.［15］李娟娟,邓伟,许泽楠,等.黄连木多倍体诱导及鉴定［J］.东北林业大学学报,2022,50(10):18-22.［16］白英豪,张晓丽,李明军.秋水仙素诱导丹参多倍体研究进展［J］.江苏农业科学,2018,46(5):18-20.［17］SAKRAN M A,ALMHEMED K,TAMER S.Study the indicators of germination and growth of some species of weeds under different thermal conditions［J］.International Journal of Research Studies in Agricultural Sciences,2020,6(9):1-5.［18］吴彦秋,叶睿华,吕享,等.秋水仙素诱导杜鹃兰原球茎产生多倍体［J］.植物生理学报,2017,53(3):407-412.［19］许陶瑜,田洪岭,郭淑红,等.药用植物多倍体育种研究进展［J］.山西农业科学,2021,49(3):392-394.［20］洪陈洁,林晗,吴承祯.植物多倍体特征与诱导及其应用［J］.武夷学院学报,2014,33(2):1-6.
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备注/Memo
第一作者简介：朱庆松（1976-），男，硕士，副教授，现主要从事植物生理和遗传育种等研究工作。E-mail：zqs43@126.com.责任作者：刘松虎（1977-），男，硕士，副教授，现主要从事植物生理与遗传育种等研究工作。E-mail：Shliu2012@126.com.基金项目：国家自然科学基金资助项目（12305399）；河南省科技攻关资助项目（242102230129）。收稿日期：2024-09-23