YUAN Renwen,MA Xiling,LIU Lin,et al.Inhibition Effect of Aqueous Extract of Mustard on Watermelon Wilt and Effects on Leaf Protective Enzyme Activities of Plants[J].Northern Horticulture,2020,44(03):36-43.[doi:10.11937/bfyy.20192016]
芥菜浸提液对西瓜枯萎病的抑制效果及对植株叶片保护酶活性的影响
- Title:
- Inhibition Effect of Aqueous Extract of Mustard on Watermelon Wilt and Effects on Leaf Protective Enzyme Activities of Plants
- Keywords:
- mustard; watermelon wilt; incidence; protective enzyme activity
- 文献标志码:
- A
- 摘要:
- 以“黑美人”西瓜和本地芥菜笋为试验材料，共设5个浓度芥菜浸提液处理，浓度分别为6.25、12.50、25.00、50.00 mg·mL-1，以0 mg·mL-1为对照（CK），采用盆栽试验研究芥菜浸提液对西瓜枯萎病的抑制效果及对植株叶片保护酶活性的影响。结果表明：在一定浓度范围内，芥菜浸提液对西瓜枯萎病病菌有一定的抑制效果，其中12.50 mg·mL-1处理效果较好，发病率较CK降低了13.33%，病情指数较CK降低12.50%；同时芥菜浸提液处理促进了西瓜植株的生长量，植株株高、茎粗、干质量、鲜质量相比于CK均有显著提高，其中12.50 mg·mL-1处理促进效果最好，当浓度超过25.00 mg·mL-1，植株生长量会逐渐降低,表现出低浓度促进高浓度抑制的变化规律；芥菜浸提液均可提高西瓜叶片SOD、POD、CAT、APX活性，其中12.50 mg·mL-1处理效果较好，SOD、POD、CAT活性分别比CK显著增加了12.61%、28.88%、9.91%，但随着处理浓度升高，保护酶活性呈现下降趋势，也表现出低促高抑的规律；此外，浸提液处理还显著降低了西瓜叶片MDA含量，提高了植株叶片中H2O2含量和可溶性糖含量。综上所述，一定浓度的芥菜浸提液可降低西瓜枯萎病的发病率，提升植株抵御逆境的能力，促进西瓜幼苗的生长。
- Abstract:
- ‘Black beauty’ watermelon and local mustard shoots were used as experimental materials，a pot experiment was conducted to study the inhibition effect of mustard extract on watermelon fusarium wilt and the effect on the activity of protective enzymes in plant leaves.A total of 5 mustard extracts with concentration of 6.25,12.50,25.00,50.00 mg·mL-1,0 mg·mL-1 were used as control (CK).The results showed that,in a certain concentration range,the mustard extract had a certain inhibitory effect on watermelon fusarium wilt bacteria,among which 12.50 mg·mL-1 had a better treatment effect,the incidence of disease was 13.33% lower than CK,and the disease index was 12.50% lower than CK.At the same time,the mustard extract treatment promoted the growth of watermelon plants,and the plant height,stem diameter,dry weight and fresh weight were significantly improved compared with CK,among which 12.50 mg·mL-1 treatment had the best effect.When the concentration exceeded 25.00 mg·mL-1,the plant growth gradually decreased,showing the change law of low concentration promoting high concentration inhibition.All the extracts of mustard could improve the activity of SOD,POD,CAT and APX in watermelon leaves,among which 12.50 mg·mL-1 had a good treatment effect.SOD,POD and CAT activity increased significantly by 12.61%,28.88% and 9.91%,respectively,compared with CK.In addition,the extract treatment also significantly reduced the MDA content in watermelon leaves,and increased the content of H2O2 and soluble sugar in plant leaves.In summary,a certain concentration of mustard extract could reduce the incidence of watermelon fusarium wilt,improve the ability of plants to resist adversity,and promote the growth of watermelon seedlings.
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备注/Memo
第一作者简介：袁仁文（1994-），男，硕士研究生，研究方向为蔬菜作物与土壤微生物的互作关系。E-mail:1743807148@qq.com.责任作者：范淑英（1963-），女，本科，教授，现主要从事蔬菜栽培生态与环境调控等研究工作。E-mail:fansy12@126.com.基金项目：国家自然科学基金资助项目（31360487）；江西省自然科学基金资助项目（20132BAB204016）。收稿日期：2019-08-01