LIU Qiong,ZHANG Xinlong,FAN Shuying.Preliminary Research on Antifungal Effect of Extracts From Garland Chrysanthemum on Watermelon Blight[J].Northern Horticulture,2016,40(14):114-118.[doi:10.11937/bfyy.201614028]
茼蒿提取物对西瓜枯萎病病菌抑制效果的初步研究
- Title:
- Preliminary Research on Antifungal Effect of Extracts From Garland Chrysanthemum on Watermelon Blight
- 文献标志码:
- A
- 摘要:
- 以茼蒿干粉为试材，设置0、MIC、EC50和MFC 4种浓度，通过测定培养液的电导率、菌丝中总糖含量、蛋白质含量、核酸泄露以及菌丝干湿样质量的变化，研究了茼蒿提取液对西瓜枯萎病菌抑制作用的影响。结果表明：加入茼蒿提取物的菌悬液电导率明显增大，其中浓度为EC50时的液体培养基中相对电导率最大，达到0.27 mS?cm-1；菌丝总糖和可溶性蛋白质含量均下降。此外，菌丝生物量也明显下降，MFC处理下湿样质量较对照减少64.34%，干样质量较对照减少78.63%。
- Abstract:
- Taking garland chrysanthemum powder as material，the effect of extracts from garland chrysanthemum with different concentration of 0、MIC、EC50 and MFC on watermelon blight was explored by measuring the conductivity of conidial suspension,mycelium contents (total sugar and soluble protein content),leakage of mycelial nucleic acid,mycelial change of dry and wet weight.The results showed that the conductivity increased significantly after the addition of extracts from garland chrysanthemum to conidial suspension and the relative conductivity of liquid medium was 0.27 mS?cm-1 in the treatment EC50.Developing for a period of time，total sugar and soluble protein content in the mycelia were lower,in addition,mycelial biomass remarkablely decreased with wet weight 64.34% and dry weight 78.63% less than the control under MFC treatment.
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备注/Memo
第一作者简介：刘琼(1991-)，女，硕士研究生，研究方向为植物保护。E-mail:1174679741@qq.com.责任作者：范淑英（1963-），女，本科，教授，研究方向为蔬菜栽培生态与环境调控。E-mail:fansy12@126.com.基金项目：国家自然科学基金资助项目（31360487）；江西省自然科学基金资助项目（20132BAB204016）。