[1]王小敏,樊苏帆,吴文龙,等.利用正交实验优选蓝莓“比洛克西”组织培养条件[J].北方园艺,2020,44(03):28-35.[doi:10.11937/bfyy.20192298]
　WANG Xiaomin,FAN Sufan,WU Wenlong,et al.Optimum Tissue Culture Conditions of Blueberry ‘Biloxi’ by Orthogonal Test[J].Northern Horticulture,2020,44(03):28-35.[doi:10.11937/bfyy.20192298]

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利用正交实验优选蓝莓“比洛克西”组织培养条件

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷期数: 2020年03 页码: 28-35 栏目: 出版日期: 2020-02-15

Title:: Optimum Tissue Culture Conditions of Blueberry ‘Biloxi’ by Orthogonal Test

作者:: 王小敏1; 樊苏帆1; 吴文龙1; 李维林2; （1.江苏省中国科学院植物研究所，江苏南京 210014；2.南京林业大学林学院，江苏南京 210037）

Author(s):: WANG Xiaomin1; FAN Sufan1; WU Wenlong1; LI Weilin2; (1.Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing,Jiangsu 210014;2.Academy of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037)

关键词:: 南方高丛蓝莓; “比洛克西”; 组织培养; 正交实验

Keywords:: southern high-bush blueberry; ‘Biloxi’; tissue culture; orthogonal test

DOI:: 10.11937/bfyy.20192298

文献标志码:: A

摘要:: 以南方高丛蓝莓“比洛克西”半木质化茎段为外植体，利用正交实验方法，高效优选其组织培养最佳条件。结果表明：外植体的最佳处理方法为先用75%乙醇消毒45 s，再用10% NaClO消毒10 min，然后把茎段接种到含WPM+ZT 1.00 mg·L-1的初代诱导培养基中；增殖培养的最佳培养基为WPM+6-BA 1.00 mg·L-1+ZT 2.00 mg·L-1+IBA 0.25 mg·L-1，壮苗培养的最佳培养基为WPM+ZT 0.50 mg·L-1+IBA 0.50 mg·L-1；生根诱导的最佳培养基质为WPM+蛭石∶椰糠（V 1∶1）+活性炭3.00 g·L-1+IBA 0.500 mg·L-1。

Abstract:: The semi-hardwood stem segments of southern high-bush blueberry ‘Biloxi’ were used as explants,and the optimum conditions for tissue culture were optimized by orthogonal test.The results showed that the best disinfection method for explants was to disinfect them with 75% ethanol for 45 seconds,then 10% NaClO for 10 minutes,and then put stems into WPM+ZT 1.00 mg·L-1 medium；the optimum medium for propagation culture was WPM+6-BA 1.00 mg·L-1+ZT 2.00 mg·L-1+IBA 0.25 mg·L-1；the optimum medium for strong seedling culture was WPM+ZT 0.50 mg·L-1+IBA 0.50 mg·L-1；and the optimum medium for rooting was WPM+vermiculite∶coconut coir (volume ratio 1∶1)+activated carbon 3.00 g·L-1+IBA 0.500 mg·L-1.

参考文献/References:

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备注/Memo

第一作者简介：王小敏（1980-），女，硕士，副研究员，现主要从事蓝莓与黑莓引种利用等研究工作。E-mail:xmwang525@163.com.责任作者：吴文龙（1964-），男，本科，研究员，现主要从事黑莓与蓝莓种质资源利用与开发等研究工作。E-mail:1964wwl@163.com.基金项目：江苏省科技资助项目（BE2019399）；江苏现代农业产业技术体系资助项目（JATS[2019]465）；江苏省农业科技自主创新资金资助项目（CX(17)1004）。收稿日期：2019-09-18

更新日期/Last Update: 2020-02-26