PEI Shuai,YAN Meiling,DONG Yewen,et al.Effect of Root Restriction on Sugar Quantity and Activities of Sucrose-metabolizin Enzymes in ‘Red Globe’ Grape[J].Northern Horticulture,2017,41(06):25-30.[doi:10.11937/bfyy.201706005]
根域限制对“红地球”葡萄果实糖含量和蔗糖代谢相关酶活性的影响
- Title:
- Effect of Root Restriction on Sugar Quantity and Activities of Sucrose-metabolizin Enzymes in ‘Red Globe’ Grape
- 文献标志码:
- A
- 摘要:
- 以3年生的“红地球”葡萄为试材，测定其果实中葡萄糖、果糖和蔗糖含量以及蔗糖代谢相关酶-可溶性酸性转化酶(SAI)、可溶性中性转化酶(SNI)、细胞壁转化酶(CBAI)和蔗糖合成酶(SSⅡ)活性，以研究葡萄果实糖类积累与蔗糖代谢相关酶活性的关系。结果表明：果实成熟期根域限制栽培果实的可溶性总糖含量明显高于对照；与对照组相比，根域限制组葡萄糖含量提高了12.8%，果糖含量提高了3.4%，蔗糖含量提高了20.0%，总糖含量提高了10.8%。说明根域限制栽培显著提高了“红地球”葡萄果实成熟期的葡萄糖、蔗糖和总糖含量，糖含量的提高受到了SAI、SNI、CBAI、SSⅡ活性的调控；根域限制栽培显著提高了转色期SAI和CBAI的活性和成熟期SNI和SSⅡ的活性。
- Abstract:
- The content of sucrose,glucose,fructose and the activities of sucrose-metabolizin enzymes (soluble acid invertase,soluble neutral invertase,cell wall-bound acid invertase,sucrose synthase and sucrose phosphate synthase) in the grape berry of ‘Red Globe’ were measured.This study aimed to explore the effect of root restriction on sugar metabolism and the activities of sucrose-metabolizin enzymes.The results showed that,the total soluble sugar content under root restriction was significantly higher than the control during grape berry maturing.Compared with the control group,the glucose content under root restriction increased by 12.8%,fructose content increased by 3.4%,sucrose content increased by 20.0%,total sugar content increased by 10.8%.It showed that,root restriction significantly improved the content of glucose,sucrose and total sugar during ‘Red Globe’ grape berry maturing The addition of soluble sugar content was controlled by activities of SAI,SNI,CBAI,SSⅡ.Root restriction greatly increased the activities of SAI and CBAI at the turning color stage and increased the activities of SNI and SSⅡ at the late stage of fruit development.
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备注/Memo
第一作者简介：裴帅（1990-），男，河南南阳人，硕士研究生，研究方向为葡萄栽培与酿酒。E-mail:1191665683@qq.com.责任作者：王振平（1965-），男，陕西绥德人，博士，研究员，硕士生导师，研究方向为葡萄栽培与酿酒。E-mail:dr.wangzhp@163.com.基金项目：国家现代产业体系技术体系资助项目（CARS-30-ZP-8）。