GAO Jinqiu,SONG Yang,SUN Lijuan,et al.Establishment a Common Stable Salt-tolerance Cell Lines of Sunflower (Helianthus annuus L.)[J].Northern Horticulture,2016,40(18):111-114.[doi:10.11937/bfyy.201618027]
通用稳定向日葵耐盐细胞系的建立
- Title:
- Establishment a Common Stable Salt-tolerance Cell Lines of Sunflower (Helianthus annuus L.)
- 文献标志码:
- A
- 摘要:
- 以10个向日葵品种为试材，利用细胞悬浮技术，研究了培养耐盐细胞系的最佳条件。结果表明：切取向日葵7日龄无菌苗子叶与胚轴接种于6-BA 05 mg?L-1+NAA 05 mg?L-1+NaCl 50 mmol?L-1+蔗糖40 g?L-1，pH 55~70的MS培养基中，培养温度为28 ℃，14 d后取其1 g愈伤组织接种于添加 NaCl 175 mmol?L-1 的30 mL液体培养基中，振荡培养，7 d继代1次，即可获得稳定耐盐细胞系。该方案不仅适合油葵也适用食葵。
- Abstract:
- Taking 10 varieties of Helianthus annuus L.as test materials，using the cell suspension technology，the optimum conditions for cultivation of salt-tolerant cell lines were studied.The results showed that cutting out the seven-day sunflower′s cotyledons and hypocotyls and inoculating in 6-BA 05 mg?L-1+NAA 05 mg ?L-1+NaCl 50 mmol?L-1+sucrose 40 g?L-1，pH 55-70 MS medium and culturing in temperature at 28 ℃ for two weeks.Then cutting 1 g the callus and put them in the 30 mL liquid medium which contained NaCl 175 mmol?L-1，shaking and subculturing every seven days，the stable salt-tolerant cell lines were obtained.This scheme was not only applicable for oil sunflower but also for eating sunflower.
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备注/Memo
第一作者简介：高金秋(1976-),女,硕士,讲师,研究方向为植物分子生物学。E-mail:wawa760824@126.com.基金项目：吉林省教育厅科学技术研究资助项目（2010-220）；白城师范学院重点资助项目（2014）。