WU Li-na.Study on the Loose Callus Induction and Cell Suspension Culture of Lonicera japonica[J].Northern Horticulture,2012,36(20):107-109.
金银花疏松愈伤组织诱导及细胞悬浮培养研究
- Title:
- Study on the Loose Callus Induction and Cell Suspension Culture of Lonicera japonica
- 文章编号:
- 1001-0009（2012）20-0107-03
- Keywords:
- Lonicera japonica; loose callus; cell suspension culture
- 分类号:
- S 567.7+9
- 文献标志码:
- B
- 摘要:
- 以“巨花一号”金银花为试材，研究了外植体、培养基对金银花疏松愈伤组织的诱导以及初始接种量和蔗糖浓度对金银花悬浮细胞生长的影响。结果表明：幼叶最易诱导愈伤组织，其诱导及增殖的最适培养基为MS+2,4-D 2.0 mg/L+KT 0.5 mg/L;细胞悬浮培养中愈伤组织最适初始接种量为60 g/L，培养基中蔗糖浓度为35 g/L时细胞生长最好。
- Abstract:
- Taking ‘Juhua No.1’ as the material,the effects of explant,medium on the loose callus induction of L.japonica were investigated，as well as the effects of primary inoculums size and sucrose concentration in the medium on the growth of suspension culture cell were studied.The results showed that the best explant which induced the callus was the young leave and its optimal medium was MS+2.0 mg/L 2,4-D+0.5 mg/L KT.The best primary inoculums size of callus was 60 g/L and the cell of L.japonica growed best when sucrose concentration was 35 g/L in suspension culture.
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备注/Memo
作者简介：武丽娜（1977-），女，河北石家庄人，硕士，讲师，研究方向为分子生物学。E-mail：xtwulina1977@sina.com.