ZHANG Shuihua,JIANG Yonggang,ZHAO Hongyan.Microwave-assisted Extraction and Antioxidant Activity of Polysaccharides From Lactarius volemus Fr.[J].Northern Horticulture,2016,40(14):135-138.[doi:10.11937/bfyy.201614034]
多汁乳菇多糖的微波辅助提取工艺及其清除DPPH自由基研究
- Title:
- Microwave-assisted Extraction and Antioxidant Activity of Polysaccharides From Lactarius volemus Fr.
- 关键词:
- 多汁乳菇; 多糖; 微波; DPPH自由基清除
- 文献标志码:
- A
- 摘要:
- 以多汁乳菇为试材，研究了液料比、微波功率、提取时间、提取次数对多糖提取率的影响，并采用正交实验优化了工艺条件，对提取产物进行了DPPH自由基清除研究。结果表明：对多糖提取率影响最大是微波功率，其次是液料比，提取时间影响最小；优化工艺条件为液料比20∶1 mL?g-1，微波功率480 W，提取时间4 min，提取3次。在此条件下多糖提取率达6.41%。多糖对DPPH自由基有一定的清除作用，证明多汁乳菇多糖具有一定的抗氧化活性。
- Abstract:
- Taking Lactarius volemus Fr.as the raw material，the effects of extraction conditions such as ratio of liquid-solid，microwave time，microwave power and time of extraction were investigated by one-factor tests and orthogonal test，and the extraction polysaccharides analyzed with DPPH? test.The results showed that the microwave power affected the yield markably，the second was ratio of liquid-solid，and microwave time had the least influence；the optimum coditions were determined as follows：ratio of liquid-solid of 20∶1 mL?g-1，microwave power of 480 W，microwave time of 4 min and times of extraction of 3 times.Under the condition，the yield was up to 6.41%.Polysaccharides exhibited antioxidant effects in 2,2′-diphenyl-1-picrylhydrazyl(DPPH?) radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
参考文献/References:
[1]朱培欣,梅余霞,梁运祥.一种虫草多糖的体外免疫学活性[J].食品科技,2013,38(9):25-28. [2]王君巧,聂少平,余强,等.黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用[J].食品科学,2012,33(23):274-277. [3]NIE S P，ZHANG H，LI W J,et al.Currentdevelopment of polysaccharides from Ganoderma：Isolation,structure and bioactivities[J].Bioactive Carbohydrates and Dietary Fibre，2013,1(1):10-20. [4]ZHU L N，LUO X，TANG Q J,et al.Isolation，purification，and immunological activities of alow-molecular-weight polysaccharide from the lingzhi or reishi medicinal mushroom Ganoderma lucidum (higher basidiomycetes)[J].International Journal of Medicinal Mushrooms,2013,15(4):407-414. [5]刘佳,高敏,殷忠.贵州野生多汁乳菇氨基酸成分及致小鼠突变性实验[J].预防医学情报杂志，2007,23(2):230-232. [6]AHMET C，ZLEM F，ERTU R S.Nutritional composition of some wild edible murshrooms[J].Urkish Journal of Biochemistry，2009,34(1):25-31. [7]胡先运,王传明,江家志,等.多汁乳菇的研究及应用[J].北方园艺,2014(18):157-160. [8]王心诗,邰丽梅,吴素蕊,等.响应面法优化水提铜色牛肝菌多糖工艺[J].中国食用菌，2015,34(1):60-64. [9]张斌,刘莹,王益,等.金针菇菌渣中多糖的微波辅助提取工艺及其抗氧化活性研究[J].天然产物研究与开发，2015,27(1):120-125. [10]陈景藻.现代物理治疗学[M].北京:人民军医出版社,2001:186-195.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(14):133.
[3]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(14):148.
[4]罗 琴,林惠昆,管春平,等.正交实验法优化提取二型鳞毛蕨多糖工艺的研究[J].北方园艺,2013,37(22):146.
LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(14):146.
[5]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(14):128.
[6]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(14):156.
[7]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(14):148.
[8]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(14):154.
[9]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(14):166.
[10]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(14):135.
备注/Memo
第一作者简介：张水花（1980-），女，硕士，副教授,研究方向为天然产物化学及保健食品。E-mail：z_shh@163.com.基金项目：云南省科技厅研究基金资助项目（2013FD047）。