WEI Xin,WEI Yongxiang,LIU Cheng,et al.Mutagenic Effect on Tissue Culture Plants and Nutritional Bowl Plants of Blueberry Induced by 60Co-γ Irradiation[J].Northern Horticulture,2016,40(10):6-9.[doi:10.11937/bfyy.201610002]
60Co-γ辐射对越橘组培苗和营养钵苗的诱变效应
- Title:
- Mutagenic Effect on Tissue Culture Plants and Nutritional Bowl Plants of Blueberry Induced by 60Co-γ Irradiation
- Keywords:
- blueberry; 60Co-γ; mutation effects
- 文献标志码:
- A
- 摘要:
- 以 ‘斯巴坦’及‘蓝丰’越橘组培苗、1年生‘斯巴坦’及‘蓝丰’和‘奥尼尔’越橘营养钵苗为试材，采用7个剂量的60Co-γ射线辐射处理，观察比较不同辐射处理条件下组培苗的生长植株比例、株高和增殖系数及营养钵苗的株高、茎粗和成活率。结果表明：随着60Co-γ辐射剂量的增加，组培苗的生长植株比例、株高和增殖系数呈下降趋势。‘蓝丰’组培苗辐照剂量大于50 Gy时生长受到严重抑制，‘斯巴坦’辐照剂量大于70 Gy时生长受到严重抑制；‘斯巴坦’和‘奥尼尔’株高和茎粗随辐射剂量的增加总体上呈下降趋势，‘蓝丰’茎粗随辐射剂量的增加总体上呈下降趋势，但株高随辐射剂量的增加总体上呈上升趋势。
- Abstract:
- Taking the tissue culture plants of ‘Bluecrop’，‘Spartan’ and the nutritional bowl plants of ‘Bluecrop’，‘Spartan’，‘O’Neal’ trees as materials.Seven 60Co-γ doses were selected to irradiate the growth ratio，plant height，multiplication constant of tissue culture plant and the plant height，stem diameter，survival rate of nutritional bowl plants were investigated．The results showed that with the increment of the 60Co-γ iradiation dose，the growth ratio，plant height，multiplication constant of tissue culture plant were showed a decling trend.The growth of tissue culture plant for ‘Bluecrop’ were inhibited on more than 50 Gy irradiation dose，The growth of tissue culture plant for ‘Spartan’ were inhibited on more than 70 Gy irradiation dose；the plant height and stem diameter of nutritional bowl plants for ‘Spartan’ and ‘O’Neal’ were showed a decling trend.The stem diameter of nutritional bowl plants for ‘Bluecrop’ were showed a decling trend，but the height showed a rising trend.
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备注/Memo
第一作者简介：魏鑫（1982-），男，硕士，助理研究员，研究方向为蓝莓种质资源与栽培生理。E-mail：run2010@163.com.责任作者：杨艳敏（1975-），女，本科，副研究员，研究方向为小浆果种质资源。E-mail：yymzcb@163.com.基金项目：科技部科技支撑计划资助项目（2013BAD02B04-04）；辽宁省科技厅农业攻关计划资助项目（2014204007）。