FU Jing-jing,ZHANG Zhi-dong,SUN Hai-yue,et al.Study on Embryo Culture of Blueberry Seeds[J].Northern Horticulture,2013,37(18):97-100.
越橘种子胚培养技术研究
- Title:
- Study on Embryo Culture of Blueberry Seeds
- 文章编号:
- 1001-0009(2013)18-0097-04
- Keywords:
- blueberries; breeding; seeds; germination
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以“蓝丰”越橘为试材，研究了其自然授粉果实中不同发育阶段种子的培养效果。结果表明：成熟越橘种子在1/2改良WPM+赤霉素600 mg/L萌发培养基上培养效率为32.0%；幼嫩种子在1/2MS发育培养基上培养30 d之后转入萌发培养基的两步培养法的培养效率为19.0%；发育期42～50 d果实中的幼嫩种子培养效率为16.3%～19.0%。在发育培养过程中用（2±2）℃ 20 d、（25±2）℃ 10 d或（25±2）℃ 10 d、（2±2）℃ 20 d 2种处理的培养效率分别为23.1%和28.8%。另外在发育期50 d的成熟果实中成熟种子、幼嫩种子和发育不良种子分别占52.1%、32.4%和15.5%。
- Abstract:
- Taking blueberry ‘Bluecrop’ as material,the natural pollination seed germination efficiency at different fruit development stages were studied.The results showed that the mature blueberry seed germination efficiency in medium composition of 1/2WPM+GA3600 mg/L was 32.0%；young seed germination efficiency at two steps germination medium composition of 1/2MS and 30 d development cultivate was 19.0%；the young seed from fruit development phases of 42~50 d seed development efficiency was 16.3%~19.0%.The development cultivate in two kinds of dispose (2±2)℃ 20 d,(25±2)℃ 10 d and (25±2)℃ 10 d,(2±2)℃ 20 d seed germination efficiency was 23.1% and 28.8% respectively.At fruit development stage of 45 d,the mature,young and stunting seeds was 52.1%,32.4% and 15.5% respectively.
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备注/Memo
第一作者简介：富晶晶（1987-），女，硕士研究生，研究方向为果树育种。E-mail:1014199885@qq.com.
责任作者：张志东（1962-），男，硕士，教授，硕士生导师，现主要从事果树育种和生物技术等研究工作。E-mail:currant1985@163.com.
基金项目：吉林省财政厅育种资助项目（2012004）；公益性行业（农业）科研专项资助项目（201103037）；吉林省科技厅资助项目（20080713）；吉林省教育厅资助项目（吉教科合字2008309）。
收稿日期：2013-04-15