HOU Xiaojie,LIANG Kuijing,CONG Rizheng,et al.Study on the Inhibitory Action of Apple Ring Spot in the Storage Period[J].Northern Horticulture,2015,39(23):115-119.[doi:10.11937/bfyy.201523032]
贮藏期苹果轮纹病拮抗菌的筛选研究
- Title:
- Study on the Inhibitory Action of Apple Ring Spot in the Storage Period
- 文献标志码:
- A
- 摘要:
- 以苹果、姜、蒜为试材，采用对峙培养法研究了健康苹果果实、姜、蒜内的可培养内生细菌和健康苹果植株根际土壤微生物对苹果轮纹病的拮抗作用。结果表明：根际土壤中分离到可培养细菌9种，姜、蒜、苹果内分别分离到可培养菌株5种、3种和1种；对分离到的微生物进行拮抗性测定，除土2对苹果轮纹病菌菌丝生长无抑制效果外，其余菌株都对苹果轮纹病菌有抑菌效果，且从根际土壤中分离到的微生物对苹果轮纹病菌具有拮抗性的所占比例较大，为44.4%；其次为姜和蒜，所占比例分别为27.8%和16.7%。所有菌株中除蒜1②，土1和土5与对照比较抑菌效果差异不显著外，其余菌株抑菌效果差异都显著。所有菌株中抑制效果最好的为姜4，其真菌菌落直径只有（30.78±0.49）mm，其次为土4，真菌菌落直径为（35.12±0.13）mm。
- Abstract:
- Apple,garlic and ginger were used as test materials,the confrontation culture method was aolopted to study the antagonism effect from culturable endophytic bacteria within healthy apple fruit，ginger，garlic，and the rhizosphere soil microorganisms in healthy apple plant for the apple ring spot.The results showed that,nine kinds of bacteria in rhizosphere soil can be cultured；the number of strains isolated from ginger，garlic and apple was 5，3 and 1，respectively.All of the isolated microorganisms except ‘soil-2’ obtained from soil had the inhibition ability and isolates from the rhizosphere soil had greater proportion of antagonism，44.4%；followed by ginger and garlic，the proportion was 27.8% and 16.7%.All strains except ‘garlic 1 ②’，‘soil-1’ and ‘soil-5’ had significant difference comparing with CK.The ‘ginger 4’ had the best inhibitory effect，the diameter of fungal colonies was only (30.78±0.49) mm，‘soil-4’ was following，fungal colony diameter was (35.12±0.13) mm.
参考文献/References:
[1]百度百科.苹果轮纹病[EB/OL].http://wapbaike.baidu.com/view/230696.html. [2]LI G X,SHEN Y B.Study on the infection mechanism of apple ring rot disease [J].Journal of Fruit Trees，2007,24(1):16-20. [3]荔克让,张华,李晓云.苹果果实轮纹病及其防治[J].中国沙漠,2000,7(20):184-186. [4]王光华.生防细菌产生的拮抗物质及其在生物防治中的作用[J].应用生态学报,2004,15(6):1100-1104. [5]百度百科.根际微生物[EB/OL].http://wapbaike.baidu.com/view/446359.html. [6]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:25-27. [7]宫春波,杨伟,刘永红,等.鲜姜汁抑菌效果的测定[J].中国调味品,2005(9):50-53. [8]陈洪生.大蒜素提取条件的优化及其抑菌活力的研究[J].食品工业科技,2007,28(4):87-89. 〖LL〗[9]陈祥贵,裴炎,彭红卫.蜡状芽孢杆菌S-1菌株抗真菌物质的分离纯化[J].四川工业学院学报,2002(2):60-63. [10]范青,田世平,蒋爱丽,等.采摘后果实病害生物防治拮抗菌的筛选和分离[J].中国环境科学,2001，21(4):313-316. [11]鄂华.非寄主植物内生菌防治桉树青枯病研究[D].保定：河北农业大学,2009. [12]汪茜,胡春锦.真菌病害拮抗菌的筛选及其对多种植物病原真菌的拮抗活性测定[J].广西农业科学2010,41(7):675-678. [13]黄海东,李晓燕,杨红鹏,等.龙胆内生菌的分离鉴定及其对苹果腐烂病病菌的拮抗作用[J].食品工业科技,2012(6):217-218. [14]陈亮,刘君丽.丁香菌酯对苹果树腐烂病的防治[J].农药,2009,4 (6):402-404. [15]邓振山,赵龙飞,王薇薇,等.银杏内生真菌的分离及其对苹果腐烂病病原菌的拮抗作用[J].西北植物学报,2009,29(3):608-613. [16]乔红萍,宏丽丽,康振生.小麦内生细菌及其对根部主要病原真菌的抑制作用[J].应用生态学报,2006,17（3):690-694. [17]史光瑚.我国水果业的发展和几点建议[J].果树科学,1998,15(2):97-102. [18]侯保林,王江柱,朱杰华,等.苹果果实轮纹病的发生和防治[J].中国果树,1998(2):42-43.
相似文献/References:
[1]赵增锋,曹克强.苹果轮纹病害流行研究及防控[J].北方园艺,2012,36(01):127.
ZHAO Zeng-feng,CAO Ke-qiang.Epidemiological Studies and Prevention and Control of Apple Ring Rot[J].Northern Horticulture,2012,36(23):127.
[2]雷琼,马文哲.53%多菌灵?中生菌素可湿性粉剂防治苹果轮纹病药效试验[J].北方园艺,2012,36(17):129.
LEI Qiong,MA Wen-zhe.Efficacy Test of Carbendazim Zhongshengmycin 53% WP on Controlling Apple Ring Rot[J].Northern Horticulture,2012,36(23):129.
[3]沈倩,胡同乐,曹克强.不同化学药剂对苹果轮纹病保护作用持效期快速测定方法的建立[J].北方园艺,2015,39(10):111.[doi:10.11937/bfyy.201510027]
SHEN Qian,HU Tong-le,CAO Ke-qiang.Rapid Method to Evaluating the Protective Duration of Different Fungicides Against Botryosphaeria dothidea on Apple[J].Northern Horticulture,2015,39(23):111.[doi:10.11937/bfyy.201510027]
[4]王洪涛,曲恒华,王英姿,等.鸡粪沼液与戊唑醇减量混用对苹果轮纹病的防效及苹果品质的影响[J].北方园艺,2018,42(18):57.[doi:10.11937/bfyy.20180750]
WANG Hongtao,QU Henghua,WANG Yingzi,et al.Effect of Biogas Slurry of Chicken Manure Mixed With Tebuconazole Reduction on Botryosphaeria dothidea and Apple Quality[J].Northern Horticulture,2018,42(23):57.[doi:10.11937/bfyy.20180750]
备注/Memo
第一作者简介：侯晓杰（1981-），女，博士，副教授，现主要从事园林植物教学与科研等工作。E-mail：houxiaojie23@163.com.基金项目：衡水市科学技术研究与发展计划资助项目（14064）；河北省高等学校青年拔尖人才计划资助项目（BJ201406）。