SHEN Qian,HU Tong-le,CAO Ke-qiang.Rapid Method to Evaluating the Protective Duration of Different Fungicides Against Botryosphaeria dothidea on Apple[J].Northern Horticulture,2015,39(10):111-115.[doi:10.11937/bfyy.201510027]
不同化学药剂对苹果轮纹病保护作用持效期快速测定方法的建立
- Title:
- Rapid Method to Evaluating the Protective Duration of Different Fungicides Against Botryosphaeria dothidea on Apple
- 文章编号:
- 1001-0009（2015）10-0111-05
- Keywords:
- apple white rot; chemical control; effective duration
- 分类号:
- S 432.1；S 48
- 文献标志码:
- A
- 摘要:
- 以“富士”品种的苹果幼果和2年生枝条为试材，采用孢子萌发法，建立不同化学药剂对苹果轮纹病菌保护作用持效期的快速检测方法，研究80%代森锰锌可湿性粉剂和80%多菌灵可湿性粉剂在室内外条件下保护作用的持效期。结果表明：采用菌龄为10～15 d的苹果轮纹病菌所产生的孢龄为1～3 d的分生孢子，在28℃恒温黑暗条件下萌发6 h后镜检孢子萌发率为适宜的孢子萌发体系。在室内和室外条件下，2种供试药剂在苹果幼果和2年生枝条上对轮纹病菌的保护作用均随时间的延长而降低，但相同条件下80%代森锰锌可湿性粉剂的保护作用优于80%多菌灵可湿性粉剂。室内条件下，80%代森锰锌可湿性粉剂在幼果和2年生枝条上的保护作用防效高于70%的持续时间为3 d，7 d后的防效仅为30%左右；在室外条件下，80%代森锰锌可湿性粉剂在幼果和2年生枝条上的保护作用随时间延长下降明显，但在幼果表面的防效下降速度显著慢于在2年生枝条表面的下降速度。该方法为快速评价不同化学药剂保护作用的持效期提供了手段。
- Abstract:
- Fruitlets and two years of branches of ‘Fuji’ apple were used to establish a rapid method based on conidial germination to evaluate the protective duration of different fungicides against apple white rot in this study.And it used the rapid method to measure the protective duration of 80% mancozeb WP and 80% carbendazim WP.The results showed that conidia appering within 3 days from fruitlets innoculated for 10-15 days were most suitable for testing.The cultivation time was 6 hours at 28℃ in the dark wet environment.Under indoor and field conditions，protective effcets of the two fungicides were descend with the extension of time on fruitlet and branch surface，but the duration of 80% mancozeb WP was longer than 80% carbendazol WP under the same the conditions.Under indoor conditions，protection effect of 80% mancozeb WP was higher than 70% for the 3 days on the surface of fruitlets and two years of branches，was only about 30% after 7 days；under field conditions，protective effcet of 80% mancozeb WP were descend obviously，but the drop speed on the surface of fruitlets was slower on two years of branches significantly.This rapid method based on conidial germination to measure the protective duration of different fungicides against apple white rot could be used in the future.
参考文献/References:
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备注/Memo
第一作者简介：沈倩（1988-），女，河北易县人，硕士研究生，研究方向植物病害流行与综合防治。E-mail：shenqian429@126.com.