XU Bin,PENG Lixia,PAN Wen,et al.Comparative Study on Methods for Testing Pollen Viability of Camellia azalea[J].Northern Horticulture,2015,39(13):88-90.[doi:10.11937/bfyy.201513026]
杜鹃红山茶花粉活力测定方法的比较研究
- Title:
- Comparative Study on Methods for Testing Pollen Viability of Camellia azalea
- 文章编号:
- 1001-0009（2015）13-0088-03
- 分类号:
- S 685.14
- 文献标志码:
- A
- 摘要:
- 以4年生嫁接的杜鹃红山茶花粉为试材，采用MTT法、TTC法和离体萌发法，研究了不同测定方法对杜鹃红山茶花粉活力的影响。结果表明：不同测定方法对杜鹃红山茶花粉活力测定结果影响较大，其中，离体萌发法是杜鹃红山茶花粉活力的最佳检测方法，30℃条件下花粉萌花最适培养基为2.5%琼脂+100 g/L蔗糖+10 mg/L硼酸，适宜统计的培养时间为4 h，花粉萌发率达58.10%，Ca2+对花粉萌发起抑制作用；MTT染色得出92.5%的杜鹃红山茶花粉具有活力,明显高于离体萌发法，而TTC法由于染色不清晰，不适合用于杜鹃红山茶花粉活力检测。
- Abstract:
- Taking 4-year-old grafted Camellia azalea as test material,effects of different methods on the viability of pollens collected from Camellia azalea were investigated，using the MTT staining method,TTC staining method and pollen germination in vitro method. The results showed that the viability of pollens was associated with the testing method,the pollen germination in vitro was the optimum method to determine pollen viability of C.azalea. The pollen germinating rate reached the highest on the media supplemented with 2.5% agar，100 g/L sucrose and 10 mg/L boric acid under 30℃ after 4 hours.However，adding the Ca2+ on the media would prevent the pollen germinating.92.5% pollen had viability in MTT solution，significantly higher than by pollen germination in vitro.TTC staining method was unsuited for detection the pollen viability of C.azalea，for its dying unclearly and lower viability rate.
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备注/Memo
第一作者简介：徐斌（1974-），男，博士，教授级高级工程师，现主要从事观赏植物研究与开发利用等工作。E-mail:xubin@sinogaf.cn.基金项目：国家“十二五”农村领域国家科技计划子研究资助项目（2012BAD01B0703-2）。