[1]张恩平,段瑜,张淑红,等.番茄总黄酮两种提取工艺优化及比较研究[J].北方园艺,2015,39(09):105-108.[doi:10.11937/bfyy.201509029]
　ZHANG En-ping,DUAN Yu,ZHANG Shu-hong,et al.Comparison and Optimization of Two Kinds of Extraction Technology of Flavonoid in Tomatoes[J].Northern Horticulture,2015,39(09):105-108.[doi:10.11937/bfyy.201509029]
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番茄总黄酮两种提取工艺优化及比较研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年09 页码: 105-108 栏目: 出版日期: 2015-05-15
Title:
Comparison and Optimization of Two Kinds of Extraction Technology of Flavonoid in Tomatoes
文章编号:
1001-0009（2015）09-0105-04
作者:
张恩平; 段瑜; 张淑红; 谭福雷
（沈阳农业大学 园艺学院，辽宁 沈阳 110866）
Author(s):
ZHANG En-ping; DUAN Yu; ZHANG Shu-hong; TAN Fu-lei
（College of Horticulture，Shenyang Agricultural University，Shenyang，Liaoning 110866）
关键词:
番茄; 总黄酮; 提取工艺
Keywords:
tomato; total flavonoid; extraction process
分类号:
S 642.9
DOI:
10.11937/bfyy.201509029
文献标志码:
A
摘要:
以番茄果实为研究对象，用黄酮含量作为提取工艺的指标，采用传统加热法和超声辅助法提取番茄总黄酮，并比较2种提取工艺效果；通过单因素试验与正交实验相结合，研究提取时间、提取温度、料液比、提取次数等对番茄总黄酮含量的影响。结果表明：番茄总黄酮的最佳提取工艺为超声辅助法：50%乙醇，提取时间为20 min，提取温度60℃，料液比为1∶40 mg/mL；在最佳提取条件下，番茄总黄酮的含量为576.95 mg/100g。
Abstract:
Taking fruit of tomatoes as research object，the content of flavonoid indicators as the evaluation standard.The total flavonoid of tomato was extracted and compared by using conventional heating and ultrasonic assisted method.Extracted by univariate time，extraction temperature，solid-liquid ratio and extract frequency were conducted to study the flavonoid content of tomato through by single factor experiment and orthogonal experiment.The results showed that the optimum extraction process of flavonnoid in tomato was ultrasound-assisted method：50% ethanol，axtraction time was 20 minutes ，extraction temperature was 60℃，solid-liquid ratio was 1∶40 mg/mL.Under optimal conditions，the content of total flavonoid in tomato was 576.95 mg/100g.

参考文献/References:


[1]杜娟,崔瑞峰,王春枝.不同施肥处理对番茄品质的影响[J].上海蔬菜,2011(3):73-75.
[2]Liahy R,Chafik H M,Lenucci S,et al.Antioxidant activity and bioactive compound changes during fruit ripening of hing-lycopene tomato cultivars[J].Journal of Food Composition and Analysis,2011,24:588-595.
[3]谢建华,谢明勇,聂少平,等.青钱柳中多糖的测定[J].分析试验室,2007,36(8):32-36
.[4]Sila V I,Kigel T B,Bondarenko O M.Pharmacology of total flavonoids from Euphorbia palustris L[J].Farm Zh,1973(4):67-70.
[5]张岩,曹国杰,张燕,等.黄酮类化合物的提取以及检测方法的研究进展[J].食品研究与开发,2008,29(1):154-157.
[6]李秀信,王荣花,李萍.紫苏黄酮对食用油脂抗氧化作用的研究[J].中国粮油学报,2007(2):63-4.
[7]陈丛瑾,黄克瀛,李德良,等.香椿叶总黄酮的超声波辅助提取及其清除DPPH自由基能力的研究[J].食品与机械,2007(1):76-77.
[8]王丽玲,焦必林,雪凡坤.保健功能因子一生物类黄酮[J].粮食与油脂,2004(9):18-19.
[9]王婷,张金超,陈瑶,等.6种黄酮抗氧化和抗肿瘤活性的比较[J].中国中药杂志,2007(8):715-717.
[10]李盈蕾,陈建华.荞麦总黄酮提取工艺研究[J].安徽农业科学,2008,36(6):2201-2202.
[11]郭育东,马崇坚,单斌,等.苦瓜总黄酮提取工艺研究[J].安徽农业科学,2010,38(12):6501-6502,6624.
[12]Wang Y L,Xi G S，Zheng Y C,et al.Microwave-assisted extraction of flavonoids from Chinese herb Radix puerariae(Ge Gen)[J].Journal of Medicinal Plants Research,2010,4(4):304-308.
[13]彭程程,王青山,赵国祥,等.黄酮类化合物的研究进展[J].农产品加工（学刊）,2010,208(5):39-43.
[14]王青豪,方芳,张熊禄.微波辅助提取绞股蓝黄酮工艺研究[J].食品科学,2010,31(22):150-151.[15]张春红,许宁,刘长江,等.微波辅助法提取野生软枣猕猴桃茎黄酮的工艺优化[J].食品工业科技,2011,32(12):335-337.

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备注/Memo

第一作者简介：张恩平（1971-），男，博士，副教授，研究方向为蔬菜栽培与生态。E-mail：zhangep024@163.com.

责任作者:张淑红(1973-)，女，博士，副教授，研究方向为蔬菜栽培与生态。E-mail:zhangsh024@163.com.
基金项目：国家星火计划课题资助项目（2012GA6500021）；国家科技成果转化资助项目（2012GB2B000091）；国家科技支撑计划资助项目（2013BAD20B08）。

更新日期/Last Update: 2015-08-02