[1]宰学明,张焕仕,王 欢.AM真菌和解磷真菌对低磷土壤滨梅插条生根和生长的影响[J].北方园艺,2014,38(14):1-5.
　ZAI Xue-ming,ZHANG Huan-shi,WANG Huan.Effect of AM Fungus and Phosphate-solubilizing Fungus on the Rooting,Growth of?Prunus maritima Cuttings in a Low Phosphorus Soil[J].Northern Horticulture,2014,38(14):1-5.
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AM真菌和解磷真菌对低磷土壤滨梅插条生根和生长的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年14期 页码: 1-5 栏目: 出版日期: 2014-07-30
Title:
Effect of AM Fungus and Phosphate-solubilizing Fungus on the Rooting,Growth of?Prunus maritima Cuttings in a Low Phosphorus Soil
文章编号:
1001-0009(2014)14-0001-05
作者:
宰学明1; 张焕仕2; 王 欢1
1.金陵科技学院 园艺学院，江苏 南京 210038；
2.南京野生植物综合利用研究院，江苏 南京 210042
Author(s):
ZAI Xue-ming1; ZHANG Huan-shi2; WANG Huan1
1.Horticulture Department,Jinling Institute of Technology,Nanjing,Jiangsu 210038；
2.Nanjing Institute for Comprehensive Utilization of Wild Plants,Nanjing,Jiangsu 210042
关键词:
AM真菌; 解磷真菌; 滨梅; 插条; 生根; 生长
Keywords:
AM fungus; phosphate-solubilizing fungus; Prunus maritima; cuttings; growth-promoting; phosphate nutrition
分类号:
Q 93
文献标志码:
A
摘要:
以1年生木质化滨梅枝条为供试插条，采用砂性低磷土壤为基质进行盆栽试验，分别测定了接种摩西球囊霉（Glomus mosseae）、毛霉(Mortierella sp.)及其混合菌剂后，滨梅生根、生长、磷素营养和根系激素水平的相关指标，以研究AM真菌和解磷真菌对低磷土壤滨梅插条的促生效应。结果表明：3种菌剂接种后滨梅根系吲哚乙酸（IAA）和玉米素（Zeatin）的水平极显著高于对照（P<0.01）；插条的含磷量、土壤有效磷含量及滨梅插条根际土壤磷酸酶活性极显著高于对照（P<0.01），植物的生根率、最长初生根的长度、根干重、地上部分干重、株高以及总叶面积等生根和生长指标显著优于对照（P<0.05）；AM真菌侵染率以混合菌剂接种最高，且对于滨梅插条磷素营养的改善和生根、生长的促进效应最为显著。研究表明，接种的3种菌剂改变了插条根系的激素代谢，强化了低磷土壤上滨梅插条的磷素营养，促进了插条的生根和植株生长；G.mosseae菌剂和Mortierella sp.菌剂的复合接种，对低磷土壤滨梅插条磷素营养的改善和生根、生长具有明显的互作效应。
Abstract:
Taking cuttings of one-year-old branch of Prunus maritima as test material,a pot experiment was conducted,the index of the rooting,growth,phosphate nutrition,and root hormonal readiness of Prunus maritima cuttings in a low phosphorus soil were studied after inoculating with Glomus mosseae,Mortierella sp.and the mixture of the former two fungi,to study the promoting growth effect of AM fungi and phosphate-solubilizing fungi on growth of Prunus maritima cuttings in low phosphorus soil.The results showed that,after inoculation with the 3 microbial inoculums,the total phosphate in cuttings,the available phosphate in soil and the soil phosphatase enzyme activities in the rhizosphere were very significantly higher than the control groups（P<0.01）.The rooting rate,length of the longest primary root,dry weight of roots,dry weight of shoots,plant heigh and total leaf area were superior to the controls significantly（P<0.05）.The mixed inoculum had the highest infection rate,and had significant effect on promoting growth,hestening rooting and improving phosphorus nutrition of beach plum cuttings,while Mortierella sp. was next and G.mosseae was slightly worse.These results indicated that,phosphate-solubilizing inocula effectively aggrandized the phosphorus nutrition of beach plum seedlings,and promoted the cuttings rooting and growth in a low phosphorus soil.Co-inoculation with Glomus mosseae and Mortierella sp.?agents had a significant interaction effect on improvement phosphorus nutrition,the rooting and growth of Prunus maritima seedlings in a low phosphorus soil.

参考文献/References:

[1] Kochian L V,Hoekenga o A,Pineros M A How do crop plants tolerate acid soils mechanisms of aluminum tolerance and phosphorous efficiency[J]. Annual Review of Plant Biology, 2004 , 55 : 459-493.
[2] Jain R, Saxena J,Sharma v.Effect of phosphate-solubilizing fungiAspergillus awamori S29 0n mungbean (Vigna radiata cv. RMG 492)growth[J]. Folia Microbiologica, 2012, 57(6):533-541.
[3] Zhang H S,Wu X H, Li G, et aL Interactions between arbuscularmycorrhizal fungi and phosphate-solubilizing fungus (Mortierella sp.)and their effects on Kostelelzkya virginica growth and soil enzyme activities of thizosphere and bulk soils at different

salinities[J]. Biology and Fertility of Soils,2011,47: 543-554.
[4] Niemi K, Hdggman H, Sarjala T.Effects of exogenous diamines on the interaction between ectomycorrhizal fungi and adventitious root formation in Scots pine in Vitro[J]. Tree Physiology, 2002, 22(6): 373-381.
[5] Scagel C F.Changes in cutting composition during early stages of adventitious rooting of miniature rose altered by inoculation with arbuscular myoorrhizal fungi [J]. Journal of the American Society for Horticultural Science.2004,129(5): 624-634.
[6]子建新,李敏,刘润进.菌根真菌与植物激素相互作用研究进展[J].青岛农业大学学报（自然科学版）,2009,26(1):4-7.
[7] Medina A,Jakobsen I, Vassilev N, et al.Fermentation of sugar beet waste by aspergillus niger facilitates growth and P uptake of external mycelium of mixed populations of arbuscular mycorrhizal fungi[J]. Soil Biology and Biochemistry,2007, 39(2): 485-492.
[8]李淑敏,武帆,大豆／玉米间作体系中接种AM真菌和根瘤菌对氮素吸收的促进作用[J].植物营养与肥料学报,2011,17 (1):110-116.
[9] Yan D L, Guo Y Q, Zai X M, et a1.Effects of electromagnetic fields exposure on rapid micropropagation of beach plum(Prunus maritima)[J]. Ecological Engineering, 2009, 35(4):597-601.
[10] Zai X M, Qin P, Wan S W, et aL The application of beach plum( Prunus maritima) to wasteland vegetation recovery in Jiangsu Province, China: Seedling cloning and transplantation[J]. Ecological Engineering, 2009, 35(4):591-596.
[11] Riley D,Barhr SASalt accumulation at the soybean root-soil interface[J]. Soil Science Society of America Proceedings, 1970, 34, 154-155.
[12] Phillips J M, Hayman D S.Improved procedures for clearing roots andstaining parasitic and vesicular arbuscular myoorrhizal fungi for rapid assessment of infection[J]. Trans Br Mycol Soc. 1970. 55: 158-161.
[13]何钟佩,农作物化学控制实验指导[M].北京:北京农业大学出版社,1993:60-68.
[14] Jones J R, Wolf B, Mills H A Plant Analysis Handbook [M]. lVEcro-Macro Publishing,1991:195-203.
[15]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,1999:15-26.
[16]赵兰坡,姜岩.土壤磷酸酶活性测定方法的探讨[J].土壤通报,1986.17(3):138-142.
[17] Kaldorf M, Ludwig-Muller J. AM fungi might affect the root morphology of maize by increasing indole-3 -butyric aad biosynthesis [J]. PhysiolPlantarum, 2000, 109: 58-64.
[18] Liu R J,Li M, Meng X X, et aL Effects of AM fungi on endogenoushormones in corn and cotton plants [J]. Mycosystema,2000, 19(1): 91-96.
[19]袁丽环,闫桂琴.丛枝菌根化翅果油树幼苗根际土壤微环境[J].植物生态学报,2010,34(6):678-686.
[20] Tarafdar J C,Marschner H. Phosphatase activity in the thizosphere andhyphosphere of VA mycorrhizal wheat supplied with inorganic and organic phosphorus[J]. Soil Biol Biochem,1994, 26: 387-395.
[21]宋勇春,冯固,李晓林,泡囊丛枝菌根对红三叶草根际土壤磷酸酶活性的影响[J].应用与环境生物学报,2000(6):171-175.


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备注/Memo

第一作者简介：宰学明(1968-)，男，江苏仪征人，博士，副教授，现主要从事植物菌根生理生态学等研究工作。E-mail：zaixueming680825@yahoo.com.cn.

基金项目：国家自然科学基金资助项目（31370533）；江苏省林业三新工程资助项目［lysx(2013)13］。
收稿日期：2014-03-13

更新日期/Last Update: 2014-08-07