ZHANG Hai-xia,CHEN Qing-qi,XU Jing,et al.The Inspection of UPLC-MS/MS for the Residue of Carbamate Pesticides in Vegetable[J].Northern Horticulture,2014,38(06):125-128.
超高效液相色谱-串联质谱检测蔬菜中氨基甲酸酯类农药残留
- Title:
- The Inspection of UPLC-MS/MS for the Residue of Carbamate Pesticides in Vegetable
- 文章编号:
- 1001-0009(2014)06-0125-04
- 关键词:
- 超高效液相色谱-串联质谱; 蔬菜; 氨基甲酸酯类农药; 残留
- Keywords:
- UPLC-MS/MS; vegetable; carbamate pesticides; vestigital
- 分类号:
- S 481
- 文献标志码:
- A
- 摘要:
- 以番茄、茄子、西葫芦、大白菜、甘蓝为试材，采用超高效液相色谱-串联质谱(UPLC-MS/MS)技术建立了蔬菜中12种氨基甲酸酯类农药残留的检测方法。结果表明：样品经乙腈提取，氨基固相萃取小柱净化，以waters BEH T3色谱柱分离，采用多反应检测模式对试材进行检测，外标法定量；在质量浓度1~100 μg/L范围内呈良好线性，相关系数均大于0.99。在空白基质中添加3个水平加标试验，回收率为65.2%~119.2%，相对标准偏差均小于5%。该方法灵敏度高、准确可靠、精密度好、选择性强，可以满足蔬菜中氨基甲酸酯类农药残留检测的要求。
- Abstract:
- Taking tomato,eggplant,summer squash,Chinese cabbage and cabbage as materials,twelve carbamate pesticides detecting methods in vegetable was established by utilizing UPLC-MS/MS technique.The results showed that the sample was extracted by acetonitri,purified by amino column and separated by water BEH T3 column.The detecting aims were determinate in multiple reaction monitoring(MRM)mode.External standard method was used to determine the residue contents.As a result,the detecting aims were with a good linear relationship(r2>0.99)in quality concentration range from 1 μg/L to 100 μg/L,and blank sample substrate added to three levels adding standard sample trial,the recowery ranges were from 65.2% to 119.2% with the relative standard deviations(RSDs) 5%.The method is highly sensitive and precise and selective,it can be satisfied with those detecting needs for the residue of carbamate pesticide.
参考文献/References:
[1]李崇英,白亚之,钮松召,等.食品中氨基甲酸酯类农药残留的分析方法[J].分析科学学报,2007,23(6)：723-724. [2]李志伟,梁丹,张建夫.氨基甲酸酯类农药残留分析方法的研究进展[J].华中农业大学学报，2008,27(5)：691-695. [3]文君,孙成均,缪红,等.高效液相色谱法同时测定蔬菜中5种氨基甲酸酯类农药[J].中国卫生检验杂志，2006,17(6)：964. [4]左海根,占春瑞.生姜中涕灭威及涕灭威砜的GC分析方法研究[J].分析试验室,2008(27)：311. [5]万益群,李申杰,鄢爱平.白术中有机磷及氨基甲酸酯类农药残留量的测定[J].分析科学学报,2007,23(3)：299. [6]万益群,陈宗保.土壤中多种有机磷及氨基甲酸酯类农药残留量的气相色谱-质谱法测定[J].分析科学学报,2006,22(5)：551． [7]王连珠,周昱,陈泳.果蔬中8种苯甲酰脲类农药残留量的液相色谱-串联质谱法测定[J].分析测试学报,2010(3)：285-287.
相似文献/References:
[1]刘少梅,王丽娟,切岩祥和,等.富硒蔬菜的研究进展[J].北方园艺,2014,38(11):177.
LIU Shao-mei,WANG Li-juan,KIRIIWA Yoshikazu,et al.Research Advances of Selenium-enriched Vegetable[J].Northern Horticulture,2014,38(06):177.
[2]徐学军,魏桂琴,贠文俊,等.兰州市高寒二阴地区蔬菜产业现状分析及发展对策研究[J].北方园艺,2014,38(10):163.
XU Xue-jun,WEI Gui-qin,YUN Wen-jun,et al.Vegetable Industry Situation Analysis and Development Strategies in Higher-cold and Cloudy-humid Region in Lanzhou[J].Northern Horticulture,2014,38(06):163.
[3]周 雷,王 玲,雷小涛,等.江汉平原蔬菜地频振灯下昆虫群落多样性研究[J].北方园艺,2013,37(22):119.
ZHOU Lei,WANG Ling,LEI Xiao-tao,et al.Study on the Diversity of Insect Trapped with Trapping Lamp in Vegetable Fields in Jianghan Plain[J].Northern Horticulture,2013,37(06):119.
[4]朱庆松,刘秀青.设施蔬菜二氧化碳施肥技术[J].北方园艺,2013,37(17):55.
ZHU Qing-song,LIU Xiu-qing.Carbondioxide Fertilization Technique of Facilities Vegetables[J].Northern Horticulture,2013,37(06):55.
[5]韩勇.高原春冬小麦复种蔬菜技术[J].北方园艺,2014,38(06):44.
HAN Yong.Techniques of Spring and Winter Wheat Multiple Cropping Vegetables in Plateau[J].Northern Horticulture,2014,38(06):44.
[6]梁巧玲,杨晖,赵鹂.丽水市郊蔬菜重金属含量及健康风险分析[J].北方园艺,2013,37(12):21.
LIANG Qiao-ling,YANG Hui,ZHAO Li.Analysis of Heavy Metal Contents in Vegetables of Lishui Suburb and the Health Hazard[J].Northern Horticulture,2013,37(06):21.
[7]李强.宁夏干旱风沙区日光温室不同茬次黄瓜及油菜周年丰产栽培技术[J].北方园艺,2013,37(07):40.
[J].Northern Horticulture,2013,37(06):40.
[8]马桂花.昆虫性信息素防治甘蓝害虫的应用效果[J].北方园艺,2013,37(23):133.
MA Gui-hua.Application Effect of Insect Sex Pheromone in Control of Cabbage Pests[J].Northern Horticulture,2013,37(06):133.
[9]徐晶,杨静,张海霞,等.蔬菜中拟除虫菊酯类农药残留调查[J].北方园艺,2012,36(15):142.
XU Jing,YANG Jing,ZHANG Hai-xia,et al.Investigation on Situation of Pyrethroids Pesticides Residues of Vegetables[J].Northern Horticulture,2012,36(06):142.
[10]马文哲,王文光,吴春霞,等.杨凌示范区蔬菜中重金属污染分析与评价[J].北方园艺,2012,36(17):46.
MA Wen-zhe,WANG Wen-guang,WU Chun-xia,et al.Analysis and Evaluation on Heavy Metal Pollution of Vegetables in Yangling Demonstration Zone[J].Northern Horticulture,2012,36(06):46.
备注/Memo
第一作者简介：张海霞（1980-），女，硕士研究生，研究方向为农药与渔药残留检测。E-mail：zhanhaixianj@163.com.