ZHANG Hao,CAO Hui.Effect of Drought Stress on Physiological Characteristics of Root in Malus robusta Rehd.[J].Northern Horticulture,2014,38(03):69-73.
模拟干旱胁迫对八棱海棠根系生理生化指标的影响
- Title:
- Effect of Drought Stress on Physiological Characteristics of Root in Malus robusta Rehd.
- 文章编号:
- 1001-0009(2014)03-0069-05
- Keywords:
- water stress; Malus robusta Rehd.; root; physiological indicators; PEG-6000
- 分类号:
- S 661
- 文献标志码:
- A
- 摘要:
- 以八棱海棠幼苗为试材，研究了不同浓度的PEG-6000模拟干旱胁迫对八棱海棠根系生理生化指标的影响。结果表明：不同程度干旱胁迫均使八棱海棠根系丙二醛（MDA）和游离脯氨酸（Pro）含量增多，过氧化物酶(POD)活性提高；过氧化氢酶(CAT)活性、可溶性蛋白质含量在干旱胁迫前期比对照均有不同程度的提高，随着胁迫时间的延长，CAT活性、可溶性蛋白质含量大幅度下降，且下降幅度为：20%PEG＞15%PEG＞10%PEG＞5%PEG；5%PEG、10%PEG、15%PEG胁迫CAT活性和可溶性蛋白含量虽下降，但仍高于对照水平；20%PEG胁迫使CAT活性、可溶性蛋白质含量低于对照水平，这说明干旱胁迫使植物体内正常的蛋白质合成受到抑制，根系可能已经失去了对干旱胁迫的适应性与可调控性，细胞内膜系统受到严重损伤；干旱逆境对膜系统的伤害是脂质过氧化作用增强的结果。
- Abstract:
- Taking Malus robusta Rehd.as material,PEG-6000 was used to simulate drought stress,the effect of water stress on physiological characteristics of root were studied.The results showed that the malondialdehyde(MDA) content，the free proline content and the peroxidase(POD) activity increased under different drought stress.The catalase(CAT) activity and the soluble protein content increased during early period，as the duration of drought stress prolonged，the CAT activity decreased，and the decrease order was 20%PEG＞15%PEG＞10%PEG＞5%PEG.CAT activity and soluble protein content decreased under 5% PEG，10% PEG，15% PEG stress,but still higher than the control level.20% PEG made CAT activity，soluble protein content below the control level,protein synthesis was inhibited，roots may have lost the adaptability to drought stress，and serious damage to the cell membrane system.Drought stress on the membrane system damage was the result of enhanced lipid peroxidation.
参考文献/References:
[1]Sharp R E
，
Poroyko V
，
Hejlek L G.Root growth maintenance during water deficits
：
physiology to functional genomics[J].Jounal of Experimental Botany
，
2004
，
55(407)
：
2343-2351.
[2]Francois C，Genevieve C，Jean-christophe B Molecular and physiological response to water deficit in drought-tolerant and drought-sensitive lines of sunflower[J].Plant Physiol，1998，116:319-328. [3]Banziger M，Setimela P S，Hodson D，et al.Breeding for improved drought tolerance in maize adapted to southern Africa.New direction for a diverse planet[A].Proceedings of the fourth International Crop Science Congress[C].Brisbane，Australia，2004:1-10. [4]曹慧.水分胁迫诱导苹果属植物叶片衰老机理的研究[D].北京:中国农业大学,2003. [5]Tomo’omi Kumagai.Modeling water transportation and storage in sapwood-model development and validation[J].Agri and For Meteo，2001，109:105-115. [6]赵昌杰,刘松忠,张强.果树对干旱胁迫的响应研究进展[J].中国果树,2011(4)：60-62. [7]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2003. [8]McMichael B L，Elmore C D.Proline accumulation in water stressed cotton leaves[J].Crop Science，1997,17:905-908. [9]贾利强.金沙江热河谷造林树种抗旱特性的研究[D].北京:北京林业大学,2003. [10]张守仁.叶绿素荧光动力学参数的意义及讨论[J].植物学通报,1999,16(4):444-448. [11]邱真静,李毅,种培芳.PEG胁迫对不同地理种源沙拐枣生理特性的影响[J].草业学报,2011(3):108-114. [12]杨洪强,黄天栋,柬怀瑞等.干旱胁迫对苹果幼树内源多胺水平的影响[J].西北农业大学学报,1995,4(3):93-99. [13]潘东明,潘良镇.水分胁迫对龙眼幼苗多胺的生理生化指标的影响[J].福建农业大学学报,1997,26(3):48-55. [14]Jain M，Mathur G，Koul S.Ameliorative effects of proline on saIt stress induced lipid peroxidation in cell line of groundnut (Arachis hypogeaL.)[J].Plant Cell Rep，2001，20:463-468. [15]余叔文.植物生理与分子生物[M].北京:科学出版社,1999:739-75. [16]魏良民.几种旱生植物碳水化合物和蛋白质变化的研究[J].干旱区研究,1991,8(4):38-41. [17]Cheruth A J，Ragupathi G，Paramasivam M，et al.Responses of antioxidant defense system of Catharanthus roseus (L.) G.Don.to paclobutrazol treatment under salinity[J].Acta Physiologiae Plantarum，2007，29(3)205-209. [18]曹慧,王孝威.水分胁迫下短枝型苹果叶片膜脂过氧化和保护酶系统的变化[C]∥中国园艺学会.中国园艺学会第九届学术年会论文集.中国园艺学会,2001:4. [19]李壮,孙效国,李敏.苹果属植物抗旱机制研究进展[J].安徽农业科学,2011(3):1351-1353.
相似文献/References:
[1]方 晶.高压电场对干旱胁迫下黄瓜种子萌发的影响[J].北方园艺,2014,38(13):41.
FANG Jing.Effect of High Voltage Electric Field on Germination of Cucumber Seeds Under Drought Stress[J].Northern Horticulture,2014,38(03):41.
[2]王贵平,王金政,薛晓敏,等.叶面喷施甜菜碱对干旱胁迫下苹果幼树[J].北方园艺,2014,38(12):10.
WANG Gui-ping,WANG Jin-zheng,XUE Xiao-min,et al.Effect of Spraying Glycinebetaine on Physiological Responses of Apple Young Trees Under Drought Stress[J].Northern Horticulture,2014,38(03):10.
[3]张 爽,赵 纯,董 然,等.槭叶草对PEG-6000模拟干旱胁迫的生理响应[J].北方园艺,2013,37(22):74.
ZHANG Shuang,ZHAO Chun,DONG Ran,et al.Physiological Responses of Mukdenia rossii Under PEG-6000 Drought Stress[J].Northern Horticulture,2013,37(03):74.
[4]蔡喜悦,陈晓德,刘成,等.外源钙对干旱胁迫下复羽叶栾树幼苗水分及光合特性的影响[J].北方园艺,2013,37(10):58.
CAI Xi-yue,CHEN Xiao-de,LIU Cheng,et al.Effect of Drought Stress with Exogenous Ca2+on Relative Water Content and the Characteristics of Photosynthesis of Koelreuteria paniculata[J].Northern Horticulture,2013,37(03):58.
[5]梁蕊芳,康利平,徐 龙,等.干旱胁迫对樱桃番茄幼苗叶片生长特性的影响[J].北方园艺,2013,37(23):12.
LIANG Rui-fang,KANG Li-ping,XU Long,et al.Effect of Drought Stress on Growth Characteristics of Leaves in Lycopersicom esculentum var. cerasiforme Seedlings[J].Northern Horticulture,2013,37(03):12.
[6]王少平,黄超.切花菊‘黄中黄’对干旱胁迫的生理响应[J].北方园艺,2013,37(15):64.
WANG Shao-ping,HUANG Chao.Physiological Response of Cut Chrysanthemum ‘Huangzhonghuang’ to Drought Stress[J].Northern Horticulture,2013,37(03):64.
[7]董延龙,常缨.干旱胁迫对五种观赏蕨类植物叶绿素荧光特性的影响[J].北方园艺,2013,37(15):66.
DONG Yan-long,CHANG Ying.Effects of Drought Stress on Chlorophyll Fluorescence Characteristics of Five Species of Ornamental Ferns[J].Northern Horticulture,2013,37(03):66.
[8]岳桦,石喜梅.聚乙二醇模拟干旱胁迫对兔儿伞生理特性的影响[J].北方园艺,2013,37(11):56.
YUE Hua,SHI Xi-mei.Effects of Drought Stress Simulated by PEG-6000 on Physiological Characteristics of Syneilesis aconitifolia[J].Northern Horticulture,2013,37(03):56.
[9]邹原东,韩振芹,陈秀新,等.干旱胁迫对蓝羊草渗透调节物质和抗氧化酶活性的影响[J].北方园艺,2013,37(23):71.
ZOU Yuan-dong,HAN Zhen-qin,CHEN Xiu-xin,et al.Effect of Drought Stress on Osmotic Adjustment Substance and Antioxidative Activity of Leymus chinensis[J].Northern Horticulture,2013,37(03):71.
[10]余莉琳,裴宗平,孔静,等.干旱胁迫下四种矿区生态修复植物的抗旱性研究[J].北方园艺,2013,37(12):61.
YU Li-lin,PEI Zong-ping,KONG Jing,et al.Drought Resistance of Four Plant Species in Ecological Regeneration on Mining Area Under Drought Stress[J].Northern Horticulture,2013,37(03):61.
[11]党云萍,李春霞.干旱胁迫下八棱海棠中丙二醛及质膜透性的变化[J].北方园艺,2012,36(21):33.
DANG Yun-ping,LI Chun-xia.Changes of the Relative Conductivity and MDA in Malus robusta Rehd Under Drought Stress[J].Northern Horticulture,2012,36(03):33.
备注/Memo
第一作者简介：张灏（1988-），男，硕士研究生，现主要从事果树生理等研究工作。