ZHAO Chao,DONG Ran,GU De-feng,et al.Study on Screening of Optimal Culture Substrate for Young Tissue Culture Seedlings of Phyllitis japonica[J].Northern Horticulture,2014,38(19):103-107.
对开蕨组培苗幼苗适宜栽培基质的筛选研究
- Title:
- Study on Screening of Optimal Culture Substrate for Young Tissue Culture Seedlings of Phyllitis japonica
- 文章编号:
- 1001-0009(2014)19-0103-05
- 分类号:
- S 682.35
- 文献标志码:
- A
- 摘要:
- 以1年生对开蕨组培苗为试材,用以草炭、山皮土、园土、菌渣和珍珠岩为基质,按照不同比例配置的7种栽培基质[T1(CK1):草炭;T2(CK2):山皮土;T3 V(园土)∶V(草炭)=2∶1;T4 V(园土)∶V(山皮土)=2∶1;T5 V(园土)∶V(山皮土)∶V(菌渣)=2∶1∶1;T6 V(草炭)∶V(山皮土)∶V(菌渣)=2∶1∶1;T7 V(草炭)∶V(珍珠岩)∶V(菌渣)=2∶1∶1]进行栽培适应性试验,研究了其在不同基质中的叶长、叶宽、叶面积和叶绿素的生长变化,并用隶属函数法综合评价。结果表明:T1的综合评价值最高,其次依次为T3、T6、T2、T5、T4和T7,综合草炭资源有限等因素,T3更适宜作为对开蕨组培苗幼苗的栽培基质推广应用。
- Abstract:
- Taking one-year-old tissue culture seedlings of Phyllitis japonica as the experimental material,the adaptability of P.japonica in the 7 kinds of culture substrate were studied.The 7 treatments were mixture of peat,hill-skill soil,garden soil,mushroom residue and perlite at the different volume ratio respectively,[T1(CK1):peat,T2(CK2):hill-skill soil,T3 V(garden soil)∶V(peat)=2∶1,T4 V(garden soil)∶V(hill-skill soil)=2∶1,T5 V(garden soil)∶V(hill-skill soil)∶V(mushroom residue)=2∶1∶1,T6 V(peat)∶V(hill-skill soil)∶V(mushroom residue)=2∶1∶1,T7 V(peat)∶V(perlite)∶V(mushroom residue)=2∶1∶1].The experiment was conducted in terms of its leaf length,leaf width,leaf area and chloroplast pigment content in different substrate formulas,then evaluated them by subordinate function.The results showed that,T1 had the highest comprehensive evaluation value,followed by T3,T6,T2,T5,T4 and T7.However,T3 was considered as more suitable for substrate promotion of young tissue culture seedlings of Phyllitis japonica due to the limited resources of peat.
参考文献/References:
[1]刘保东,时述武.长白山的珍稀观赏植物-对开蕨[J].中国野生植物,1991(4):37-38.
[2]刘鹏,康华靖,张志详,等.香果树(Emmenopterys henryi)幼苗生长特性和叶绿素荧光对不同光强的响应[J].生态学报,2008,28(11):5656-5664.
[3]顾德峰,李东升,王蕾,等.东亚对开蕨离体快繁的研究[J].园艺学报,2008,35(9):1373-1376.
[4]岳桦,吴妍,姜丽颖.不同栽培基质对对开蕨的影响[J].黑龙江农业科学,2011(2):66-68.
[5]连兆煌,李式军.无土栽培原理与技术[M].北京:中国农业出版社,1994:50-100.
[6]苑克俊,刘庆忠,李圣龙,等.利用数码相机测定果树叶面积的新方法[J].园艺学报,2006,33(4):829-832.
[7]张斯美.叶面积测量法[J].北京农业科技,1981(5):62-69.
[8]张治安,陈展宇.植物生理学实验技术[M].长春:吉林大学出版社,2008:66-68.
[9]陶向新.模糊数学在农业科学中的初步应用[J].沈阳农业大学学报,1982,13(2):96-107.
[10]任爽英,刘春,冯冰,等.东方百合‘Sorbonne’无土栽培基质的研究[J].北京林业大学学报,2011,33(3):92-98. [11]曹凯,佘新,赵艳艳,等.沙地番茄无土栽培基质的筛选[J].西北农林科技大学学报(自然科学版),2013,41(6):147-152.
[12]孙向丽,张启翔.4种农业有机废弃物对丽格海棠盆花的栽培效果[J].东北林业大学学报,2011,39(3):31-33. [13]郭世荣.固体栽培基质研究、开发现状及发展趋势[J].农业工程学报,2005,21(增):1-4.
[14]蒋卫杰,杨其常.小康之路?无土栽培特选项目与技术[M].北京:科学普及出版社,2008:46-52.
[15]徐文俊,程智慧,孟焕文,等.农业废弃有机物基质配方对番茄生长及产量的影响[J].西北农林科技大学学报(自然科学版),2012,40(4):127-133.
[16]田洪,王殿芝,赵占英,等.东北对开蕨的栽培[J].人参研究,1997(2):16-17.
[17]张智顺,张庆费,夏檑,等.遮阴对几种绿化植物光合特性和生长的影响[J].东北林业大学学报,2010,38(3):47-49,72.
[18]Boardman N K.Comparative photosynthesis of sun and shade plants [J].Annual Review of Plant Physiology,1977,28:355-377.
[19]Goodwin T W.The biochemistry of carotenoids [J].Plant,Chapman and Hall,1980,V(1):529.
[20]刘旭,张延龙,牛立新,等.‘西伯利亚’百合可再生栽培基质的筛选[J].西北农林科技大学学报(自然科学版),2012,40(11):179-186.
[21]宫亚军,路虹,石宝才.不同栽培基质对蚕豆苗生长的影响[J].北方园艺,2008(1):1-3.
相似文献/References:
[1]张存智,王 敏,魏 鹏.枣组培苗试管外微嫁接技术研究[J].北方园艺,2014,38(11):100.
ZHANG Cun-zhi,WANG Min,WEI Peng.Study on the Outside Tube Micrografting Techniques for the Tissue Cultured Zizpihus jujube Buds[J].Northern Horticulture,2014,38(19):100.
[2]李京,张建瑛,张妍妍,等.“美登”蓝莓组培苗移栽技术研究[J].北方园艺,2013,37(13):36.
LI Jing,ZHANG Jian-ying,ZHANG Yan-yan,et al.Study on the Transplanting Technology of Tissue Cultured Seedling of ?Blueberry ‘Blomidon’[J].Northern Horticulture,2013,37(19):36.
[3]田 河,师校欣,杜国强,等.基质及营养液对苹果矮砧组培苗移栽后生长的影响[J].北方园艺,2013,37(06):8.
TIAN He,SHI Xiao-xin,DU Guo-qiang,et al.Effect of Substrate and Nutrient Solution Supplied on Growth of Plantlets of Apple Dwarf Rootstocks in vitro [J].Northern Horticulture,2013,37(19):8.
[4]王安石,王健,林明光.红掌组培苗工厂化生产污染控制技术[J].北方园艺,2013,37(08):58.
WANG An-shi,WANG Jian,LIN Ming-guang.The Techniques of Pollution Control in Factory Production of Anthurium andraeanum Plantlets[J].Northern Horticulture,2013,37(19):58.
[5]权俊萍,戴丽娜,唐徐林,等.薰衣草组培苗驯化移栽过程中形态解剖学比较[J].北方园艺,2012,36(03):55.
QUAN Jun-ping,DAI Li-na,TANG Xu-lin,et al.Comparative Study on Morphology and Anatomy of Lavender During Transplantation and Acclimatization[J].Northern Horticulture,2012,36(19):55.
[6]钟波.洋桔梗组培苗生产关键技术研究[J].北方园艺,2012,36(16):90.
ZHONG Bo.Study on Key Technology of Production Tissue Culture Seedling of Enstoma grandiflorun[J].Northern Horticulture,2012,36(19):90.
[7]赵红霞,高文强,张利,等.杜鹃组培苗的练苗技术研究[J].北方园艺,2012,36(07):75.
ZHAO Hong-xia,GAO Wen-qiang,ZHANG Li,et al.Study on the Hardening-seedling Technology of Tissue Culture Seedlings of Rhododendron simsii Planch[J].Northern Horticulture,2012,36(19):75.
[8]张正海,张悦,李爱民,等.五味子组培苗生长优化研究[J].北方园艺,2012,36(10):133.
ZHANG Zheng-hai,ZHANG Yue,LI Ai-min,et al.Optimized Study on Growth of Tissue Culture Sprout of Schisandrachinensis(Turcz.)Baill[J].Northern Horticulture,2012,36(19):133.
[9]韦坤华,李林轩,王美英,等.山豆根组培苗的质量分析[J].北方园艺,2012,36(13):185.
WEI Kun-hua,LI Lin-xuan,WANG Mei-ying,et al.Analysis on the Ouality of Tissue-cultured Plantlets of Sophora tonkinensis Gapnep.[J].Northern Horticulture,2012,36(19):185.
[10]闻永慧,孟英,李慧敏,等.LED不同光质对白及组培苗生长及可溶性糖含量的影响[J].北方园艺,2014,38(15):58.
WEN Yong-hui,MENG Ying,LI Hui-min,et al.Effect of LED Different Light Qualities on Growth and the Content of Soluble Sugar of Bletiua striata Tissue Culture Seedlings[J].Northern Horticulture,2014,38(19):58.
[11]赵 超,董 然,顾德峰,等.不同空气湿度对对开蕨组培苗生长状况的影响[J].北方园艺,2014,38(08):24.
ZHAO Chao,DONG Ran,GU De-feng,et al.Effect of Different Air Humidity on Growth Status of Tissue Culture Seedlings of Phyllitis japonica[J].Northern Horticulture,2014,38(19):24.
备注/Memo
第一作者简介:赵超(1987-),男,硕士研究生,研究方向为园林植物资源与种质创新。E-mail:barry.zc@163.com.
责任作者:董然(1966-),女,博士,教授,研究方向为长白山园林植物研究。E-mail:Dongr999@163.com.
基金项目:国家“十二五”科技支撑计划资助项目(2012BAD22B0401);吉林省科技发展计划资助项目(20110262)。
收稿日期:2014-05-27