ZHANG Zhu-li,JIANG Yan-qin,YU Hong.Effects of Different Culture Conditions on the Adventitious Bud Regeneration of Detached Leaves of Blueberries in vitro [J].Northern Horticulture,2013,37(22):104-107.
不同培养条件对蓝浆果离体叶片不定芽再生的影响
- Title:
- Effects of Different Culture Conditions on the Adventitious Bud Regeneration of Detached Leaves of Blueberries in vitro
- 文章编号:
- 1001-0009(2013)22-0104-04
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以南方高丛蓝浆果（Vaccinium corymbosum hybrids）品种“南月”（Southmoon）优选系A18离体叶片为试材，研究了不同细胞分裂素、蔗糖浓度、叶片放置方式、暗培养时间及叶片完整性对不定芽再生的影响。结果表明：TDZ和CPPU对不定芽的诱导效果好于ZT和2ip；在培养基中添加20 g/L蔗糖较利于不定芽的再生；外植体近轴面接触培养基不定芽再生效果好；离体叶片不经过暗培养及其创伤口大均有利于不定芽再生。
- Abstract:
- The detached leaves of superior strain A18 selected from southern highbush blueberry (Vaccinium corymbosum)‘Southmoon’ were used as the explants,the effect of different cytokinin,sugar concentration,inoculation method,dark periods and artificial leaf wounds on adventitious bud regeneration was studied.The results showed that the effects of TDZ and CPPU were better than ZT and 2ip on adventitious bud regeneration;medium containing 20 g/L sugar was suitable for adventitious bud regeneration;the adaxial side in contact with the medium had greater effect;no darkness treatments and artificial leaf wounds were also favorable to regeneration.
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备注/Memo
第一作者简介：张祝丽（1985-），女，安徽安庆人，硕士研究生，现主要从事蓝浆果组培研究工作。E-mail:zhulizhang1107@163.com.
责任作者：於虹（1968-），女，博士，研究员，现主要从事小浆果类果树的引种驯化和利用研究工作。E-mail:njyuhong@vip.sina.com.
基金项目：国家农业部公益性行业（农业）科研专项资助项目（201103037）；
江苏省农业自主创新资助项目［CX(11)1011］。
收稿日期：2013-06-27