CAO Ya-qiong,ZHANG Cun-xu.Study on Induction of Embryogenic Tissue of Platycladus orientalis [J].Northern Horticulture,2013,37(06):108-110.
侧柏胚性愈伤组织的诱导研究
- Title:
- Study on Induction of Embryogenic Tissue of Platycladus orientalis
- 文章编号:
- 1001-0009（2013）06-0108-03
- Keywords:
- Platycladus orientalis; embryogenic callus; immature zygotic embryo; radicle; initiation factors
- 分类号:
- S 791.38
- 文献标志码:
- A
- 摘要:
- 以侧柏未成熟合子胚、胚根为试材，研究了基本培养基、不同外植体、植物激素、合子胚发育阶段等因素对侧柏愈伤组织诱导的影响。结果表明：未成熟合子胚、胚根在适宜的培养基上都能诱导出愈伤组织，DCR+2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.5 g/L谷氨酰胺是未成熟合子胚诱导愈伤组织的最佳培养基，诱导率高达96.67％。DCR+1.0 mg/L 2,4-D+0.1 mg/L 6-BA+0.5 g/L谷氨酰胺是胚根诱导愈伤组织的最佳培养基，诱导率高达93.33％。最佳采样时期为7月3日前后。
- Abstract:
- Taking the immature zygotic embryo and radicle of Platycladus orientalis as test materials，the effects of basic medium，different explants，plant hormones，zygotic embryo developmental stage in callus induction were studied.The results showed that immature zygotic embryo and radicle could induce on optimal medium.The optimal combination of medium for immature zygotic embryo was DCR+2.0 mg/L 2,4-D+0.2 mg/L 6-BA+0.5 g/L glutamine，the inducing rate was 96.67％.The optimal combination of medium for radicle was DCR+1.0 mg/L 2,4-D+0.1 mg/L 6-BA+0.5 g/L glutamine，the inducing rate was 93.33％.The best period of immature zygotic embryo sampling was around July 3.
参考文献/References:
[1]
郑万钧
.
中国树木志
[M].
北京
:
中国林业出版社
,1998:10
．
[2]黄洛华,龙玲,陆熙娴,等.侧柏枝叶精油的化学组成与抗蚁性[J].林业科学研究,2004,14(4):416-420． [3]蒋继宏,李晓储,陈凤美,等.芳香型植物挥发油抑菌活性的研究[J].江苏林业科技,2004(3):7-8,13． [4]桑艳朋,赵莉,王祯丽.新疆城市园林绿化树种的选择[J].新疆农垦科技,2004(6):25-27． [5]祝朋芳,罗凤霞,陈长青,等.日本落叶松胚性愈伤组织的诱导及体胚的形成[J].辽宁农业科学,2001(5):46-47. [6]贺凤美,王力,刘阳.落叶松体细胞发生研究进展[J].辽宁林业科技,2000(6):33-35. [7]郑均宝,王进茂,杜克久,等.油松体细胞无性系的建立[J].遗传学报,2000,24(1):1-6. [8]唐巍.火炬松胚性愈伤组织诱导和植株再生的研究[J].林业科学,1998,34(3):115-119. [9]金晓玲,何平.乔木树种体细胞胚胎发生的研究与应用[J].林业科学研究,2003,16(3)：343-350. [10]Li X Y,Huang F H,Gbur E F.Effect of basal medium,grow thregulators and phytagel concentration on initiation of embryogenic cultures from immarure zygotic embryos of loblolly pine[J].Plant Cell Reports,1998,17:298-301. [11]Ellis D D.Stabletrans form ation of Piceaglauca by particle accelerat ion[J].Bio Technology,1993,11:84-89. [12]翟晓巧,翟翠娟,刘新凤.木本植物体细胞胚胎发生及植株再生研究现状[J].河南林业科技,2004,24(2):7-9. [13]Becwar M R,Nagamani R,Wann S R.Initation of embryogenenic cultures and somati cembryo development in loblolly pine(Pinus taeda)[J].Can J For Res,1990,20:810-817.
相似文献/References:
[1]代月,吴雄,廉美兰,等.影响洋葱愈伤组织及胚性愈伤组织诱导的因素研究[J].北方园艺,2013,37(07):125.
DAI Yue,WU Xiong,LIAN Mei-lan,et al.Study on the Factors of Onion Callus and Embryonic Callus Induction[J].Northern Horticulture,2013,37(06):125.
[2]马丽源,张瑛,尚福强,等.油松未成熟合子胚离体培养诱导胚性愈伤组织[J].北方园艺,2012,36(07):126.
MA Li-yuan,ZHANG Ying,SHANG Fu-qiang,et al.Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulaeformis[J].Northern Horticulture,2012,36(06):126.
[3]闫 兴 富,周 立 彪,杜 茜.遮荫处理对侧柏种子萌发的影响[J].北方园艺,2009,33(05):0.[doi:10.11937/bfyy.200905069]
YAN Xing - fu,ZHOU Li - biao,DU Qian.Effects of Shading T reatments of Different Light Gradients on the Germination of Platycladusorientalis Seed[J].Northern Horticulture,2009,33(06):0.[doi:10.11937/bfyy.200905069]
[4]赵今哲,刘国彬,张鸿翎,等.植物生长调节剂对侧柏扦插繁殖的影响及不定根形成[J].北方园艺,2020,44(10):77.[doi:10.11937/bfyy.20192458]
ZHAO Jinzhe,LIU Guobin,ZHANG Hongling,et al.Effects of Plant Growth Regulator on Cutting Propagation of Platycladus orientalis Franco and Adventitious Root Formation[J].Northern Horticulture,2020,44(06):77.[doi:10.11937/bfyy.20192458]
备注/Memo
第一作者简介：曹亚琼（1987-），女，陕西延安人，在读硕士，现主要从事园林植物遗传育种工作。E-mail：843894295@qq.com.