LI Wei,AO Yan-fei,HE Wen-chao,et al.Establishment of Cotyledon in vitro Culture System and Plant Regeneration of Pod Pepper[J].Northern Horticulture,2014,38(21):91-97.
朝天椒子叶离体培养与植株再生体系的建立
- Title:
- Establishment of Cotyledon in vitro Culture System and Plant Regeneration of Pod Pepper
- 文章编号:
- 1001-0009（2014）21-0091-07
- 分类号:
- S 641.303.6
- 文献标志码:
- A
- 摘要:
- 以贵州黎平朝天椒地方种为试材，取其无菌苗子叶作外植体，采用植物固体培养基组织培养法，研究了培养基激素配比、无菌苗生理年龄、子叶部位等对朝天椒子叶的离体接种、诱导愈伤、不定芽分化、芽茎伸长、诱导生根、驯化移栽、成苗等离体培养及植株再生阶段的影响。结果表明：朝天椒子叶适宜芽诱导分化的培养基为MS+6-BA 2 mg/L+NAA 0.1 mg/L，芽分化率达100%；适宜芽伸长的培养基为MS+KT 2 mg/L，芽伸长率可达50%；适宜生根的培养基为MS无激素培养基，生根率可达44%。单个外植体平均分化含15~18个不定芽的芽丛（14~16个芽茎），出苗6~7株。从子叶外植体接种至再生苗出瓶需50~55 d，再生苗移栽成活率达98%。该研究构建了贵州朝天椒的高效离体培养植株再生体系，为朝天椒的组织快繁和遗传转化奠定了重要的基础。
- Abstract:
- Taking native species of pod pepper from Lipin county of Guizhou province as materials,cotyledons of germinated aseptic seedlings were cut as explants.Adopting the plant tissue culture method with solid medium,effects of different media of a basal nutrient medium supplemented with different auxins,cytokinins and auxin-cytokinin combinations,different physiological ages of aseptic seedlings and different parts of cotyledon on cotyledon in vitro culture system and plant regeneration,such as in vitro cotyledon inoculation,callus induction,adventitious bud differentiation,bud elongation,root induction,domestication,transplantation and growing adult plant were investigated.The results showed that the optimal medium for adventitious bud differentiation was MS+6-BA 2 mg/L+NAA 0.1 mg/L,with 100% bud differentiation percentage.The optimal medium for bud elongation was MS+KT 2 mg/L,with 50% bud elongation percentage.And the rooting medium was MS without other supplements,with 44% taking root percentage.The average of 15-18 adventitious clustered buds were differentiated by one explant.Among these adventitious buds differentiated per explant,14-16 buds were elongated,and 6-7 seedlings were obtained at last.This period was 50-55 days from cotyledon explant inoculation to regeneration seedlings.The survival percentage of regeneration seedlings after transplanting was 98%.The efficient regeneration system by in vitro culture was preliminarily established on pod pepper of Guizhou province,which laid important foundation for tissue culture in rapid propagation and genetic transformation of pod pepper in this study.
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备注/Memo
第一作者简介：李伟(1977-),男,博士,副教授,研究方向为蔬菜学教研工作。E-mail:liw21@163.com.
基金项目：贵州大学人才基金资助项目（贵大人基合字(2007)021号）;贵州省农业攻关资助项目（黔科合NY[2013]3024号）。
收稿日期：2014-07-14