ZHU Dexuan,DU Chunmei,DONG Xiwen,et al.Optimization of Fermentation Conditions for a Inorganic-Phosphorous-Solubilizing Bacterium Isolated From Cold Region[J].Northern Horticulture,2020,44(10):45-50.[doi:10.11937/bfyy.20193779]
一株寒地高效解无机磷细菌发酵条件优化
- Title:
- Optimization of Fermentation Conditions for a Inorganic-Phosphorous-Solubilizing Bacterium Isolated From Cold Region
- Keywords:
- cold region; bacterium; ability of solubilize inorganic phosphorus; fermentation condition; optimization
- 文献标志码:
- A
- 摘要:
- 为了探究解无机磷细菌B51-7最佳发酵培养条件，以发酵液初始pH、碳源、氮源和培养温度为研究对象，以钼蓝比色法测定发酵液中可溶性磷含量为评价指标，通过单因素试验和正交实验确定其发酵条件的最佳组合。结果表明：初始pH 4.5、30 ℃、10 g·L-1葡萄糖、0.1 g·L-1 蛋白胨培养条件下，菌株B51-7发酵液中可溶性磷含量最高；菌株B51-7解磷能力的最优发酵条件组合为初始pH 4.0，葡萄糖12 g·L-1，蛋白胨0.05 g·L-1，温度30 ℃。该研究为菌株B51-7解磷能力的掌握以及其在微生物磷肥的开发与利用提供了重要的参考依据。
- Abstract:
- To optimize the fermentation conditions of inorganic-phosphorous-solubilizing bacterium B51-7.The initial pH，carbon source，nitrogen source and culture temperature were choosed as the test factors.The content of soluble phosphorus in fermentation broth was determined by molybdenum blue colorimetry.The optimum combination of fermentation conditions was determined by single factor test and orthogonal test.The results showed that the highest soluble phosphorus content in the fermentation broth of strain B51-7 appeared when the initial pH 4.5，30 ℃，10 g·L-1 glucose and 0.1 g·L-1 peptone culture conditions.The optimal fermentation conditions affecting the phosphorous ability of strain B51-7 were initial pH 4.0，glucose 12 g·L-1，peptone 0.05 g·L-1，and 30 ℃.This study provided important reference basis for the development and utilization of strain B51-7 in microbial phosphate fertilizer.
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备注/Memo
第一作者简介：朱德旋（1994-），女，硕士研究生，研究方向为资源微生物开发与应用。E-mail:1601769160@qq.com.责任作者：杜春梅（1978-），女，硕士，讲师，现主要从事微生物的教学与科研等工作。E-mail:dcm_037@163.com.基金项目：佳木斯大学校长创新创业基金资助项目（XZYF2019-29）；黑龙江省教育厅基本科研业务费基础研究资助项目（2016-KYYWF-0581）；黑龙江省自然科学基金面上资助项目（C2017064）；北药与功能食品特色学科资助项目（2017）。收稿日期：2020-01-12