LIU Yang,OU Xiaohong,HU Yipu,et al.Effects of Nitrate Nitrogen on the Growth and Heterophyllin B of Pseudostellaria heterophylla[J].Northern Horticulture,2024,(7):104-111.[doi:10.11937/bfyy.20233616]
硝态氮对太子参生长与环肽B含量的影响
- Title:
- Effects of Nitrate Nitrogen on the Growth and Heterophyllin B of Pseudostellaria heterophylla
- 文章编号:
- 1001-0009(2024)07-0104-08
- Keywords:
- P.heterophylla; nitrate nitrogen; growth and development; nutrient content; heterophyllin B
- 分类号:
- S 567.53
- 文献标志码:
- A
- 摘要:
- 以太子参“施太1号”为试材，采用植物组织培养的方法，研究了硝态氮用量对太子参生长发育与环肽B含量的影响，以期为太子参组培苗培育氮素用量提供参考依据。结果表明：不加氮时，太子参植株矮小、叶片黄化、根系形成较少；添加硝态氮后太子参长势变好，株高和叶片数增加、叶片颜色转为深绿色、根系形成增多；与不加氮相比，添加硝态氮显著提升了抗氧化酶活性、增加了氮素同化酶活性及养分吸收量，当硝态氮用量为20 mmol·L-1时其生长发育最佳，当硝态氮用量为10 mmol·L-1时太子参环肽B含量及前体基因prePhHB表达均达到最高。相关性分析结果表明，太子参环肽B含量及prePhHB表达均与其氮素含量呈显著正相关，说明提高太子参氮素含量可以促进环肽B合成。综上，以10~20 mmol·L-1硝态氮作为氮源，可以促进组培太子参生长发育及环肽B积累。
- Abstract:
- The variety ‘Shitai No.1’ of Pseudostellaria heterophylla was used for experimental material.Different nitrate usages were designed to study the growth and development，as well as heterophyllin B content of P.heterophylla by tissue culture method,in order to provide reference for the nitrogen dosage in the cultivation of P.heterophylla tissue culture seedlings.The results showed that the plants of P.heterophylla with no nitrogen addition had a phenotype of short plant，leaves chlorosis，less root formation and enlongation;while with nitrate addition as N resource，the plant could grow normally with higher plant height，more leaves with dark green，more roots formation.Compared with no N addition，supplying nitrate significantly increased the activities of antioxidant enzymes and N metabolism enzymes，enhanced the mineral nutrients uptake.The best growth of P.heterophylla was achieved at supplying 20 mmol·L-1 nitrate;the highest heterophyllin B content and prePhHB expression of P.heterophylla，which were positively correlated with the N content，were both gained at supplying 10 mmol·L-1 nitrate.This means improving N content of P.heterophylla could increase the accumulation of heterophyllin B.In conclusion，supplying 10-20 mmol·L-1 nitrate as the N resource in tissue culture of P.heterophylla，could promote the growth and development as well as the accumulation of heterophyllin B.
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备注/Memo
第一作者简介：刘洋（1998-），男，硕士研究生，研究方向为中药及民族药资源分类鉴定及质量控制。E-mail：1070740800@qq.com.责任作者：周涛（1968-），女，博士，教授，现主要从事中药资源等研究工作。E-mail：taozhou88@163.com.基金项目：国家自然科学基金地区资助项目（82160716，82160728）；贵州省高层次创新型人才资助项目（黔科合平台人才［2018］5638-2）；贵州省教育厅高校科研平台资助项目（黔教技［2022］021号）；现代农业产业技术体系资助项目（CARS-21）；贵州省基础研究（自然科学）资助项目（黔科合基础-ZK［2022］一般464）。收稿日期：2023-10-23