TANG Maolin,ZHANG Yang,WANG Yayi,et al.Effects of Sodium Selenite on the Growth and Synthesis of Major Nutrients in the Mycelium of Coprinus comatus[J].Northern Horticulture,2024,(7):112-119.[doi:10.11937/bfyy.20233239]
外源硒对鸡腿菇菌丝生长及合成主要营养素的影响
- Title:
- Effects of Sodium Selenite on the Growth and Synthesis of Major Nutrients in the Mycelium of Coprinus comatus
- 文章编号:
- 1001-0009(2024)07-0112-08
- Keywords:
- Coprinus comatus; sodium selenite; selenium rich; protein; polysaccharides
- 分类号:
- S 646.9
- 文献标志码:
- A
- 摘要:
- 以1株鸡腿菇菌株Cc-900为试材，采用平板培养和液体发酵培养的方法，研究不同浓度的外源硒对鸡腿菇菌株Cc-900的生长、富硒效果和菌丝合成蛋白质和多糖的影响，以期为富硒鸡腿菇的栽培和产品的开发提供参考依据。结果表明：鸡腿菇菌丝可耐受10 mg·kg-1硒浓度的固体培养基和最大硒浓度为3 mg·L-1的液体培养基，高浓度硒对菌丝活性损伤较大；当液体培养基中硒浓度为3 mg·L-1时，菌丝干质量可达1.97 g·L-1，菌丝干粉的硒含量为38.24 mg·kg-1，其硒富集系数可达12.75，菌丝中粗蛋白和多糖含量分别为2.58%和3 081.82 μg·g-1，发酵液中可溶性蛋白质和多糖含量分别为50.98 μg·mL-1和266.38 μg·mL-1。液体发酵培养中硒浓度在3 mg·L-1以下可促进鸡腿菇菌丝的生长和硒的富集，同时促进菌丝中蛋白质的合成和释放，但会抑制菌丝中多糖的合成。
- Abstract:
- Taking a Coprinus comatus strain Cc-900 as the experimental material，plate culture and liquid fermentation culture were used to study the effects of different concentrations of exogenous selenium on the growth，selenium enrichment，and protein and polysaccharide synthesis of a Coprinus comatus strain Cc-900，in order to provide reference for the cultivation and product development of Coprinus comatus.The results showed that the optimal tolerance of Coprinus comatus mycelium to selenium concentration was at 10 mg·kg-1 in solid culture medium and 3 mg·L-1 in liquid culture medium，whereas high concentrations of selenium caused reduction of mycelial activity and significant damage to mycelial growth.On the other hand，1.97 g·L-1 mycelium dry weight，38.24 mg·kg-1 selenium content of the mycelium powder，and a selenium enrichment coefficient at 12.75 were achieved at 3 mg·L-1 selenium in liquid culture medium.Likewise，crude protein and polysaccharide contents in the mycelium was 2.58% and 3 081.82 μg·g-1，respectively，while soluble protein and polysaccharide contents in the fermentation broth was 50.98 μg·mL-1 and 266.38 μg·mL-1，respectively.The results showed that Selenium concentrations below 3 mg·L-1 in liquid fermentation culture can promote the growth and selenium enrichment of Coprinus comatus hyphae，as well as the synthesis and release of proteins，while inhibiting the synthesis of polysaccharides.
参考文献/References:
［1］石军,孙德文,陈安国.微量元素硒的生物学功能及其应用［J］.兽药与饲料添加剂,2002,7(1):34-37.［2］喻大松.陕西紫阳和青海平安富硒环境中硒分布特征及其对人体健康的影响［D］.杨凌:西北农林科技大学,2015.［3］张正伟.香菇硒富集规律与富硒香菇生产关键技术的初步研究［D］.武汉:华中农业大学,2016.［4］刘书畅,马布平,周忠发,等.富硒食用菌的研究进展［J］.食药用菌,2018,26(2):74-78.［5］谢玲,马湘豫,高楚倩,等.临沂基地不同食药用菌中6种金属元素的ICP-MS检测［J］.食药用菌,2021,29(3):222-231.［6］李素春,何生根.一种极具开发价值的珍稀菇:鸡腿菇［J］.广东农业科学,1999,26(2):26.［7］余杰,崔鹏举,崔仕超,等.鸡腿菇菌丝深层培养富硒的研究［J］.食品与发酵工业,2008,34(7):93-97.［8］秦楠,刘羽,王力宇.响应曲面法优化鸡腿菇菌丝发酵液抗菌蛋白及其抗氧化活性［J］.生物技术通报,2018,34(4):83-90.［9］吴丽军,孙小凤,张荣,等.青海不同品种春油菜对硒的吸收、积累及分配规律［J］.西北农业学报,2013,22(8):47-51.［10］李志江.考马斯亮蓝G250染色法测定啤酒中蛋白质含量［J］.酿酒,2008,35(1):70-72.［11］魏文平,任丽,王丹慧.牛奶蛋白质的测定方法优化［J］.中国乳品工业,2010,38(8):41-42,45.［12］国家卫生和计划生育委员会,国家食品药品监督管理总局.食品安全国家标准 食品中蛋白质的测定:GB 5009.5-2016［S］.北京:中国标准出版社,2017.［13］张慢,邢苏徽,千春录,等.7种食用菌的营养成分及抗氧化性分析［J］.食品科技,2022,47(6):120-126.［14］铁梅.食用菌中硒的形态分析［D］.上海:华东师范大学,2006.［15］张彩妮,邓百万,柏秋月,等.富硒条件下蜜环菌菌株硒耐受性及胞外酶生物活性的研究［J］.微生物学报,2022,62(2):640-649.［16］兰天康,顾浩峰,王燕.食用菌中主要营养素与硒元素含量的相关性分析［J］.陕西农业科学,2017,63(1):42-46.［17］李红侠,王晴,刘俊杰,等.硒对杏鲍菇菌丝生长和营养成分影响研究［J］.洛阳师范学院学报,2019,38(5):24-26.［18］吴周斌.真姬菇菌草栽培及其富硒特性的研究［D］.福州:福建农林大学,2015.［19］潘亚璐.富硒栽培对双孢蘑菇生长特性及硒形态分布的影响［D］.南京:南京农业大学,2013.
相似文献/References:
[1]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(7):158.
[2]金 硕,孔佳慧,高云婷,等.鸡腿菇生产中高效安全杀虫剂的筛选[J].北方园艺,2013,37(09):1722.
JIN Shuo,KONG Jia-hui,GAO Yun-ting,et al.Screening of High Efficiency and Safety Insecticides in Production of Coprinus comatus[J].Northern Horticulture,2013,37(7):1722.
[3]翁 梁,温 鲁.硒和钙对蛹虫草活性物质含量的影响[J].北方园艺,2012,36(22):159.
WENG Liang,WEN Lu.Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances[J].Northern Horticulture,2012,36(7):159.
[4]王文举,王振平.亚硒酸钠对“红地球”葡萄耐热性及果实品质的影响[J].北方园艺,2012,36(24):47.
WANG Wen-ju,WANG Zhen-ping.The Effect of Sodium Selenite on Heat-resistance and Berry Quality of Red Globe Grape[J].Northern Horticulture,2012,36(7):47.
[5]郑素月,李云梦,邢志伟,等.七个不同鸡腿菇菌株的酯酶同工酶和ISSR分析[J].北方园艺,2015,39(17):85.[doi:10.11937/bfyy.201517022]
ZHENG Suyue,LI Yunmeng,XING Zhiwei,et al.Analysis of Esterase Isozyme and ISSR of Seven Different Coprinus comatus[J].Northern Horticulture,2015,39(7):85.[doi:10.11937/bfyy.201517022]
[6]黄 海 洋,郭 秀 英,储 凤 丽.鸡腿菇高效立体循环栽培技术[J].北方园艺,2010,34(16):0.[doi:10.11937/bfyy.201016067]
[7]倪蕾,孙涌栋,李艳华,等.不同浓度亚硒酸钠对水培苦苣生长及营养品质的影响[J].北方园艺,2016,40(14):40.[doi:10.11937/bfyy.201614010]
NI Lei,SUN Yongdong,LI Yanhua,et al.Effect of Different Concentrations of Na2SeO3 on the Growth and Nutritional Quality of Cichorium endivia[J].Northern Horticulture,2016,40(7):40.[doi:10.11937/bfyy.201614010]
[8]石 娜,李 远 想,王 尚 堃,等.鸡腿菇玉米芯栽培培养料配方对比试验[J].北方园艺,2009,33(11):0.[doi:10.11937/bfyy.2009011086]
SHI Na,LI Yuan -xiang,W ANG Shang -kun,et al.Comparative T est of Corncob Material Formulations on Cultivation of CoprinusComatus[J].Northern Horticulture,2009,33(7):0.[doi:10.11937/bfyy.2009011086]
[9]邓 功 成,陈 江 涛.麦 秆 生 料 栽 培 鸡 腿 菇 高 产 技 术[J].北方园艺,2007,31(07):0.[doi:10.11937/bfyy.200707091]
DENG Gong - cheng,CHEN Jiang - tao.Cultivation T echnique of Coprinus conmatus in Nonfermentative Straw[J].Northern Horticulture,2007,31(7):0.[doi:10.11937/bfyy.200707091]
[10]高 美 玲,袁 成 志,张 大 秀.Na 2 SeO 3 处理对不同品种西瓜幼苗生长的影响[J].北方园艺,2007,31(11):0.[doi:10.11937/bfyy.2007011025]
GAO Mei - ling,YUAN Cheng - zhi,ZHANG Da - xiu.Effect of Na 2 SeO 3 T reatment on Growth of Watermelon[J].Northern Horticulture,2007,31(7):0.[doi:10.11937/bfyy.2007011025]
备注/Memo
第一作者简介：唐茂林（1997-），男，硕士研究生，研究方向为植物营养学。E-mail：2605445208@qq.com.责任作者：李松龄（1972-），男，硕士，研究员，现主要从事土壤肥料和农业微生物利用等研究工作。E-mail：465883114@qq.com.基金项目：青海省农林科学院创新基金资助项目（2023-NKY-01)；青海省科技特派员专项资助项目（2023-NK-P30）。收稿日期：2023-09-20