WANG Rui,ZHOU Tingting,WANG Yikai,et al.Study on the Decomposition and Transformation of Ginsenosides From Different Parts of Ginseng in Soil[J].Northern Horticulture,2023,(23):100-107.[doi:10.11937/bfyy.20232415]
人参不同部位皂苷组分在土壤中腐解转化研究
- Title:
- Study on the Decomposition and Transformation of Ginsenosides From Different Parts of Ginseng in Soil
- 文章编号:
- 1001-0009(2023)23-0100-08
- 分类号:
- S 567.51
- 文献标志码:
- A
- 摘要:
- 以人参腐解产物为试材，采用超高效液相色谱-高分辨四极杆静电场轨道阱质谱联用(UPLC-Orbitrap-MS)方法，研究了腐解对人参茎叶、须根、全根、全株4种不同部位中原人参二醇型(PPD)和原人参三醇型(PPT)皂苷组分的影响，以期为人参化感作用物质基础的深入研究提供参考依据。结果表明：在人参不同部位及其土壤腐解物中共归属鉴定相关人参皂苷19个。人参皂苷组分经土壤微生物腐解后，PPD型人参皂苷Rb1、Rb2、Rb3和Rc转化生成人参皂苷Rd、F2、Rg3、Rh2、Rg5和Rk1，PPT型人参皂苷Re、Rg1和F1转化生成Rg2、Rh1、Rk3、Rh4、F4和Rg4。人参不同部位皂苷成分组成及比例存在差异。经腐解后相应部位含有的皂苷发生了一定程度的结构转化，PPD和PPT型皂苷组分在各部位的转化规律相同，导致其腐解物中稀有皂苷组分比例升高。
- Abstract:
- Taking ginseng decomposition products as experimental materials，and ultra-high performance liquid chromatography high-resolution quadrupole electrostatic field orbital trap mass spectrometry (UPLC-Orbitrap-MS) method was applied.The effects of decomposition on the components of protopanaxadiol type (PPD) and protopanaxatriol type (PPT) saponins in four different parts of ginseng stem，leaf，fibrous root，whole root and whole plant were studied,in order to provide reference for the in-depth study of the material basis of ginseng′s chemosensory effects.The results showed that a total of 19 ginsenosides were deduced and identified in different parts of ginseng plants and its soil decomposition.After decomposition by soil microorganisms，PPD-type ginsenosides Rb1，Rb2，Rb3 and Rc were converted to ginsenosides Rd，F2，Rg3，Rh2，Rg5 and Rk1，and PPT-type ginsenosides Re，Rg1 and F1 were converted to Rg2，Rh1，Rk3，Rh4，F4 and Rg4.The composition and proportion of ginsenosides in different parts of ginseng varied.After decomposition，the ginsenosides in the corresponding parts presented a certain degree of structural transformation.And the transformation patterns of PPD and PPT-type ginsenosides were the same in all parts，resulting in a higher proportion of rare ginsenosides in the decompositions.
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备注/Memo
第一作者简介：王瑞(1999-)，女，硕士研究生，研究方向为药物分析学。E-mail：wangrui_chn@163.com.责任作者：黄鑫(1981-)，女，博士，副研究员，硕士生导师，现主要从事中药学等研究工作。E-mail：huangxinrose@163.com.基金项目：国家自然科学基金资助项目(82073969)；吉林省科技发展计划资助项目(20200504003YY)。收稿日期：2023-07-18