[1]张丹,边贞贞,杨广顺,等.人参悬浮细胞系的建立及生长特性研究[J].北方园艺,2012,36(06):99-102.
　ZHANG Dan,BIAN Zhen-zhen,YANG Guang shun,et al.Studies on Establishment and Characterization of Suspension Cells in Panax ginseng[J].Northern Horticulture,2012,36(06):99-102.
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人参悬浮细胞系的建立及生长特性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第36卷 期数: 2012年06期 页码: 99-102 栏目: 出版日期: 2012-06-16
Title:
Studies on Establishment and Characterization of Suspension Cells in Panax ginseng
文章编号:
1001-0009（2012）06-0099-04
作者:
张丹; 边贞贞; 杨广顺; 张美萍; 孙春玉; 王义
吉林农业大学 生命科学学院，吉林 长春 130118
Author(s):
ZHANG Dan; BIAN Zhen-zhen; YANG Guangshun; ZHANG Mei-ping; SUN Chun-yu
College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118
关键词:
人参; 愈伤组织; 悬浮培养
Keywords:
Panax ginsing; callus; suspension culture
分类号:
S 567.5+1
文献标志码:
A
摘要:
在建立人参悬浮细胞系的基础上,研究了碳源、氮源、磷源及植物生长调节物质对人参悬浮培养物的影响。结果表明：MS+2.0 mg/L 2,4-D+2.0 mg/L NAA+0.4 mg/L BA为最适培养基；接种量10 g/100mL,19℃,100 r/min转速条件下,人参悬浮细胞系生物量积累最大。
Abstract:
The experimental was established on the basis of the Panax ginseng suspension cells.The culture conditions of carbon source,nitrogen source,phosphate and different growth regulars were researched.The results showed that the optimal medium for suspension culture was：MS+2.0 mg/L 2,4-D+2.0 mg/L NAA+0.4 mg/L BA.On the basis of inoculation was 10 g/100 mL，culture temperature was 19℃ and rotation ratio was 100 r/min，the biomass accumulation of the Panax ginseng suspension cells was the largest.

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备注/Memo

第一作者简介：张丹（1986-）,女，在读硕士,研究方向为植物分子生物学与基因工程。E-mail：mdjzd2620@126.com。

责任作者：王义（1964-）,男，博士,教授,硕士生导师,研究方向为植物细胞工程。E-mail：wanglaoshi2007@tom.com。
基金项目：长春市科技计划资助项目（2008138）。
收稿日期：2011-12-26

更新日期/Last Update: 2014-08-30