[1]贾艳丽,韩紫薇,仇燕.新型抗菌肽对番茄灰霉病的防治效果与机理的研究[J].北方园艺,2023,(18):18-27.[doi:10.11937/bfyy.20230407]
　JIA Yanli,HAN Ziwei,QIU Yan.Novel Antimicrobial Peptide on Control of Tomato Gray Mold and Its Mechanism[J].Northern Horticulture,2023,(18):18-27.[doi:10.11937/bfyy.20230407]
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新型抗菌肽对番茄灰霉病的防治效果与机理的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年18 页码: 18-27 栏目: 出版日期: 2023-09-30
Title:
Novel Antimicrobial Peptide on Control of Tomato Gray Mold and Its Mechanism
文章编号:
1001-0009(2023)18-0018-09
作者:
贾艳丽; 韩紫薇; 仇燕
（河北科技大学 食品与生物学院，河北 石家庄 050018）
Author(s):
JIA Yanli; HAN Ziwei; QIU Yan
(College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018)
关键词:
抗菌肽; 番茄灰霉病; 防治效果; 抑菌机理; 抗氧化
Keywords:
antimicrobial peptides; tomato gray mold; control effect; antifungal mechanism; antioxidation
分类号:
S 641.2
DOI:
10.11937/bfyy.20230407
文献标志码:
A
摘要:
以番茄、抗菌肽和番茄灰霉菌为试验材料，采用平板试验和番茄盆栽植株试验，研究了抗菌肽CB和D20I-39对灰霉菌（Botrytis cinerea）的抑菌效果及抑菌机制、抗菌肽对番茄灰霉病防效及对番茄叶片的抗病、抗氧化指标的影响，以期为抗菌肽防治番茄灰霉病提供参考依据。结果表明：CB和D20I-39均能抑制灰霉菌菌丝的生长和孢子萌发且具剂量依赖效应，并造成灰霉菌胞内核酸和蛋白质的泄露；CB和D20I-39处理后灰霉菌菌丝呈扁平状、皱缩变细，且随浓度增大菌丝皱褶越发严重甚至出现破裂。番茄盆栽试验显示，预防试验的CB和D20I-39防治效果分别为96.37%±0.13%和83.41%±0.17%；治疗试验的CB和D20I-39的防治效果则分别为14.86%±0.35%和59.74%±0.23%。外源喷施抗菌肽能够提高苯丙氨酸解氨酶（PAL）、超氧化物歧化酶（SOD）活性和游离脯氨酸（Pro）含量，降低丙二醛（MDA）含量。新型抗菌肽CB和D20I-39通过破坏细胞膜抑制灰霉菌，降低番茄灰霉病发病率和病情指数。抗菌肽CB和D20I-39对番茄灰霉病具有良好的防治效果。
Abstract:
Tomato,antimicrobial peptides (AMPs) and tomato Botrytis cinerea were used as test materials.The antifungal effects of CB and D20I-39 on tomato B.cinerea and the relative mechanisms were investigated by plate experiments.The control effects of AMPs on tomato leaves infected by gray mold and the indexes of disease resistance and antioxidation were analyzed by pot experiments,in order to provide reference for AMPs to control tomato gray mold.The results showed that both CB and D20I-39 could inhibit mycelial growth and spore germination of B.cinerea dosedependently and caused leakage of intracellular nucleic acid and protein in B.cinerea.After treatment of CB and D20I-39,the mycelia of B.cinerea were flattened,shrunken and narrowed.And as the concentration increased,the mycelial folds became more severe and even rupture occurred.In pot experiments,the preventive test demonstrated the control effects of CB and D20I-39 were 96.37%±0.13% and 83.41%±0.17%,respectively and the therapeutic test exhibited the control effects of CB and D20I-39 were 14.86%±0.35% and 59.74±0.23%,respectively.AMPs could increase the activities of PAL,SOD and the levels of Pro,whereas decreased MDA content.The results indicated that the novel AMPs CB and D20I-39 inhibited B.cinerea by destorying cell membrane to exert the antifungal activity,and reduced the incidence and diseases index of tomato gray mold.CB and D20I-39 had good control effects on tomato gray mold.

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备注/Memo

第一作者简介：贾艳丽（1996-），女，硕士研究生，研究方向为植物病理学。E-mail：279773299@qq.com.责任作者：仇燕（1977-），女，博士，教授，硕士生导师，现主要从事天然产物及其生物学功能等研究工作。E-mail：qiuyan2015@126.com.基金项目：京津冀三地联合攻关资助项目（19YFSLQY00100）；河北省重点研发计划资助项目（22322801D）。收稿日期：2023-02-13

更新日期/Last Update: 2023-11-22