LUO Taimin,XIANG Mei,XU Wen.Effects of Seed Soaking With Vitamin B6 on Seed Germination and Seedling Growth of Pepper Under Low Temperature Stress[J].Northern Horticulture,2023,(18):9-17.[doi:10.11937/bfyy.20224625]
维生素B6浸种对低温胁迫下辣椒种子萌发和幼苗生理特性的影响
- Title:
- Effects of Seed Soaking With Vitamin B6 on Seed Germination and Seedling Growth of Pepper Under Low Temperature Stress
- 文章编号:
- 1001-0009(2023)18-0009-09
- Keywords:
- Capsicum annuum L.; seedlings; vitamin B6; seed soaking; low temperature stress
- 分类号:
- S 641。3
- 文献标志码:
- A
- 摘要:
- 以辣椒为试材,采用浸种处理方法,研究不同浓度维生素B6溶液浸种对4 ℃低温处理下辣椒种子萌发的影响,以及对12 ℃低温处理下两叶一心期的幼苗生长的影响,以期为进一步研究维生素B6诱导辣椒抗性及调控机制提供参考依据。结果表明:4 ℃低温胁迫下经100 mg·L-1的维生素B6溶液浸种后的辣椒种子其发芽率、发芽势、发芽指数显著高于对照;经100 mg·L-1维生素B6溶液浸种后的辣椒种子长至两叶一心,在12 ℃低温下处理后辣椒幼苗的丙二醛含量和超氧阴离子产生速率显著降低,根系活力提高;经80 mg·L-1维生素B6溶液浸种后的辣椒种子长至两叶一心,在12 ℃低温处理后辣椒幼苗根长、株高、鲜质量高于对照;经40 mg·L-1维生素B6溶液浸种后的辣椒种子长至两叶一心,在12 ℃低温下处理后辣椒幼苗的过氧化氢酶、过氧化物酶、超氧化物歧化酶活性、幼苗根系活力均提高。因此,适宜浓度的维生素B6溶液浸种有助于降低低温给辣椒种子发芽、幼苗生长带来的伤害,提高其低温抗性。
- Abstract:
- Taking pepper as test material,a seed soaking treatment method was used,the effects of different concentrations of vitamin B6 solution on pepper seed germination under 4 ℃ low temperature treatment and seedling growth under 12 ℃ low temperature treatment were studied, in order to provide reference for further study of vitamin B6 induced resistance and regulation mechanism of pepper.The results showed that the germination rate,germination potential and germination index of pepper seeds soaked in 100 mg·L-1 vitamin B6 solution under 4 ℃ low temperature stress were significantly higher than those of the control;the pepper seeds soaked in 100 mg·L-1 vitamin B6 solution grew to have two leaves.The malondialdehyde content and O2 production rate of pepper seedlings treated at 12 ℃decreased significantly,and the root activity increased;the pepper seeds soaked in 80 mg·L-1 vitamin B6 solution grew to two leaves,and the root length,plant height and fresh weight of the seedlings were higher than that of the control group after being treated at 12 ℃;the pepper seeds soaked in 40 mg·L-1 vitamin B6 solution grew to two leaves,and the activities of CAT,POD and SOD,and the root activity of pepper seedlings were increased after being treated at 12 ℃.Therefore,the appropriate concentration of vitamin B6 solution could help to reduce the damage of low temperature to pepper seed germination and seedling growth,and improve its low temperature resistance.
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备注/Memo
第一作者简介:罗太敏(1996-),女,硕士研究生,研究方向为蔬菜逆境调控生理及分子机制。E-mail:1838195635@qq.com.责任作者:须文(1975-),女,博士,教授,现主要从事蔬菜生长发育调控等研究工作。E-mail:wxu1@gzu.edu.cn.基金项目:贵州省“千”层次人才培养资助项目(贵大“千”层次[2018]02号);贵州省高等学校设施蔬菜工程研究中心平台建设资助项目(黔教技[2022]040 号);贵州大学引进人才资助项目(贵大人基合字[2016]48 号)。收稿日期:2022-11-14