LI Deping,CHEN Yiming,HE Yuanqin,et al.Optimization of Tissue Culture and Rapid Propagation System for Dendrobium crystallinum Rchb.f[J].Northern Horticulture,2023,(14):111-117.[doi:10.11937/bfyy.20223873]
晶帽石斛组培快繁体系优化
- Title:
- Optimization of Tissue Culture and Rapid Propagation System for Dendrobium crystallinum Rchb.f
- Keywords:
- Dendrobium crystallinum Rchb.f; basic medium; auxin; orthogonal test
- 文献标志码:
- A
- 摘要:
- 以晶帽石斛为试材，采用正交实验方法，研究了基本培养基、生长素种类对晶帽石斛组培苗生长的影响，分析了NAA、水解乳蛋白、MS培养基有机成分、活性炭4个因素、3个水平条件下晶帽石斛组培苗壮苗培养的生长表现，从而确定最佳培养基组合，以期优化晶帽石斛组培快繁体系。结果表明：Anderson培养基和生长素NAA更适合组培苗生长，植株生长旺盛，叶片大而绿；正交实验结果表明，MS培养基有机成分是影响晶帽石斛组培苗生长的主要因素；其次生长素NAA主要影响植株的株高、茎粗、叶片数、叶长，但〖JP+2〗对生根量无影响；水解乳蛋白主要影响组培苗生根量，对株高、茎粗、叶片数以及叶长无影响；活性炭在晶帽石斛组培苗生长过程中对组培苗生长影响效果最小。试验最终确定晶帽石斛组培苗生长的最适培养基为以Anderson为基本培养基附加0.10 g?L-1活性炭、1.00 mg?L-1 NAA、0.05 g?L-1水解乳蛋白、1倍MS培养基有机成分，并附加30 g?L-1蔗糖。
- Abstract:
- Useing Dendrobium officinale as the test material,the effects of basic culture medium and auxin types on the growth of Dendrobium officinale tissue culture seedlings were studied.Based on this,orthogonal experimental methods were used to analyze the growth performance of Dendrobium officinale tissue culture seedlings under four factors: NAA,hydrolyzed milk protein,MS medium organic components,activated carbon,and three levels of conditions,in order to determine the optimal medium combination,to optimize the tissue culture and rapid propagation system of Dendrobium officinale.The results showed that Anderson medium and auxin NAA were more suitable for the growth of tissue culture seedlings，the plants grew vigorously，and the leaves were large and green；the results of the orthogonal test showed that the organic components of MS medium were the main factors affecting the growth of Dendrobium crystallinum Rchb.f capsicum tissue culture seedling.Auxin NAA mainly affected plant height，stem diameter，number of leaves，and leaf length，but had no effect on rooting amount；hydrolyzed milk protein mainly affected the rooting amount of tissue culture seedlings，and no effect on plant height，stem diameter，number of leaves and leaf length.Activated carbon had the least effect on the growth of tissue culture seedlings of Dendrobium crystallinum Rchb.f during the growth process.The test finally determined that the optimal medium for the growth of Dendrobium crystallinum Rchb.f caps tissue culture seedlings was,Anderson as the basic medium，supplemented with 0.10 g?L-1 activated carbon，1.00 mg?L-1 NAA，0.05 g?L-1 hydrolyzed milk protein，and 1 time MS medium organic ingredients，and add 30 g?L-1 sucrose.This study lay the foundation for the establishment of a high efficiency tissue culture and rapid propagation system for Dendrobium crystallinum Rchb.f.
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备注/Memo
第一作者简介：李得萍（1997-），女，硕士研究生，研究方向为植物组织培养与育种。Email:1505442752@qq.com.责任作者：刘艳军（1970-），男，硕士，高级实验师，现主要从事园艺植物组织培养和分子育种等研究工作。Email:liuyanjun00a@126.com.收稿日期：2022-09-20