ZHANG Tianjing,SUN Wensong,SHEN Baoyu,et al.Identification and Biological Characteristics of Root Rot Pathogen of Ligusticum jeholense[J].Northern Horticulture,2023,(14):118-124.[doi:10.11937/bfyy.20225201]
辽藁本根腐病病原菌鉴定及生物学特性
- Title:
- Identification and Biological Characteristics of Root Rot Pathogen of Ligusticum jeholense
- 文献标志码:
- A
- 摘要:
- 以具典型根腐病症状的辽藁本病根组织为试材，采用组织分离法对病原菌进行分离、纯化，依据柯赫氏法则测定其致病性，通过形态学观察结合EF-1α基因片段序列分析，明确辽藁本根腐病病原菌及其生物学特性，以期为该病的防控提供参考依据。结果表明：该病原菌为茄镰孢菌（Fusarium solani），不同培养基对辽藁本根腐病菌的生长量和菌落致密性有显著影响，在PDA培养基生长最快，7 d菌落直径达85 mm，菌落致密；病菌以蔗糖为碳源时，菌丝生长速率最快，可溶性淀粉次之，对乳糖利用较差；最适宜的氮源为牛肉浸膏，其次为尿素和硝酸钾；在20~30 ℃温度范围生长较快，最适温度为30 ℃；最适pH为6；光照处理对菌丝也有影响，在全黑暗培养条件下菌落生长最快。
- Abstract:
- Taking diseased plants with typical symptoms as test materials,pathogen isolation was carried out by means of diseased tissue isolation method.Koch′s rule was used to prove the pathogenicity of fungal strain,in order to explore the main pathogens of Ligusticum jeholense root rot disease and its biological characteristics.The results showed that based on the morphological observation and sequence analyses of translation elongation factor EF-1α, Fusarium solani was identified as the pathogen.The biological characteristics showed that different media had significant effects on the growth and mycelial density of pathogen.The suitable medium for Fusarium solani growth was PDA,the colony diameter reached 85 mm in 7 days,and the colonies were dense.The growth of mycelia with sucrose as carbon source was faster than that with soluble starch，and the ability of pathogen to utilize lactose was poor.The most suitable nitrogen source was beef extract，followed by urea and potassium nitrate.The suitable temperature for the mycelium growth was 20-30 ℃，the optimum temperature was 28-30 ℃.The optimum pH was 6.The light treatment had an influence on the growth of mycelium，suitable light condition for the mycelium growth was 24 hour continuous dark.
参考文献/References:
[1]于英,张永刚,刘国辉,等.辽藁本主要生物学特性研究[J].吉林农业大学学报,2005,27(4):401-404,407.[2]卢星原,孙启时,查美娜,等.辽藁本化学成分的分离与鉴别[J].沈阳药科大学学报,2010,27(6):434-439.[3]国家药典委员会.中国药典(一部)[M].北京:中国医药科技出版社,2020.[4]孙文松,李玲,于春雷,等.辽藁本生产研究现状及发展对策[J].辽宁省农业科学,2018(5):60-63.[5]刘莹,李旭,孙文松.辽藁本常见病虫害及综合防控技术[J].园艺与种苗,2021,41(4):35-36.[6]董汉松.植病研究法[M].北京:中国农业出版社,2012.[7]方中达.植病研究法[M].3版,北京:中国农业出版社,1988.[8]LESLIE J F，SUMMERELL B A.The Fusarium laboratory manual[M].USA:Blackwell Publishing,2006.[9]胡兰,姜钰,徐秀德.基于形态学和EF-1α序列特征的我国高粱子粒寄藏镰孢菌种群鉴定[J].作物杂志,2013(4):129-132.[10]COLLINS C H，LYNE P M，GRANGE J M,et al.Collins ＆ Lyne′s microbiological methods[M].Oxfod:Oxford University Press Inc,2004.[11]张雁,袁泉,刘莉莉,等.拮抗尖孢镰刀菌的蓝莓菌根真菌的筛选和鉴定[J].巢湖学院学报,2019,21(3):78-88.[12]程鸿燕,张大琪,黄斌,等.微生物菌肥对熏蒸剂处理后土壤微生态的影响研究进展[J].农药学学报,2020,22(5):734-741.[13]穆向荣,马逾英,杨枝中,等.药用植物根腐病防治的研究进展[J].中药与临床,2014,5(2):5-8.[14]姜竹,李晶.中药材土传病害生物防治研究进展[J].现代农业科技,2009(24):152-153.[15]JANG C S，LIM J H，SEO M W，et al.Direct detection of Cylindrocarpon destructans，root rot pathogen of ginseng by nested PCR from soil samples[J].Mycobiology，2010,38(1)：33-38.[16]SUMMERELL B A，SALLEH B，LESLIE J F.A utilitarian approach to Fusarium identification[J].Plant Disease，2003，87(2):117-128．[17]TEHRANI M M，NASR E M，MOUSAVI A，et al.Regulation of related genes promoting resistant in Iris against root rot disease， Fusarium oxysporum f.sp.gladioli[J].Genomics,2020,112(5)：3013.[18]高芬,任小霞,王梦亮,等.中草药根腐病及其微生物防治研究进展[J].中国中药杂志,2015,40(21)：4122-4126.[19]闫文雪,石延霞,柴阿丽,等.豇豆根腐病病原菌鉴定[J].植物保护学报,2019,46(3)：717．[20]陈为京,郭峰,陈建爱,等.连作花生根腐病镰刀菌分离与对峙培养[J].花生学报，2018，47(2):47-51.[21]罗文富,喻盛甫,贺承福,等.三七根腐病病原及复合侵染的研究[J].植物病理学报,1997,27(1)：85-91.[22]冯茜.川芎根腐病病原鉴定、生物学特性和同工酶研究[D].雅安:四川农业大学,2008.[23]李勇,刘时轮,杨成民,等.北京地区柴胡根腐病的病原菌鉴定[J].植物病理学报,2009，39(3)：314-317.[24]XIAO R F,ZHU Y J,WANG J P，et al.First report of Fusarium solani causing root rot on Levisticum officinale in China[J].Plant Disease，2017,101(4)：630.[25]连芸芸,李焕宇,李惠霞,等.甘肃省庆阳市辣椒镰孢菌根腐病病原鉴定及生物学特性[J].甘肃农业大学学报,2021,56(5):55-61,68.[26]柏玉晶,张贞明,姚玉玲,等.紫花苜蓿根腐新病原厚垣镰孢菌生物学特性研究[J].草原与草坪,2016,36(6):49-53.[27]林清洪,林光荣,曾碧玉,等.蝴蝶兰叶基腐病病原鉴定及生物学特性[J].热带作物学报,2007,28(4):93-97.[28]朱孟烽,吴伟怀,贺春萍,等.咖啡腐皮镰孢黑果病病原鉴定及其生物学特性测定[J].热带作物学报,2021,42(3):822-829.[29]耿丽华,白庆荣,王建设,等.甜瓜镰孢根腐病菌生物学特性研究[J].中国农学通报,2010,26(7):229-232.[30]贵晓荷.紫花苜蓿根腐病病原镰刀菌的生物学特性研究[D].兰州:兰州大学,2019.[31]沈丽淘,李平,王学贵,等.山药根腐病菌(Fusarium solani)的生物学特性[J].四川农业大学学报,2012,30(3):313-318.[32]刘丽云,刘晓林,刘志恒,等.辣椒根腐病菌生物学特性研究[J].沈阳农业大学学报,2007(1):54-58.[33]姚锦爱,黄鹏,陈峰,等.建兰茎腐病原菌尖孢镰刀菌的生物学特性研究[J].福建农业学报,2018,33(2)：190.[34]杨静美,陈健,罗金棠,等.番木瓜茄病镰刀菌的生物学特性研究[J].中国热带农业,2011(1)：56.[35]XU C G,ZHANG H J,ZHOU Z S,et al.Identification and distribution of Botryosphaeriaceae species associated with blueberry stem blight in China[J].European Journal of Plant Pathology,2015，143(4):737.
相似文献/References:
[1]沈清清,刘 芳,胡 彦.药用植物根腐病病原菌研究进展[J].北方园艺,2014,38(11):187.
SHEN Qing-qing,LIU Fang,HU Yan.Research Progress of Pathogens of Root Rot Disease on Medicinal Plants[J].Northern Horticulture,2014,38(14):187.
[2]曹利祥,苏卫国,栾业亮,等.不同药剂处理对无花果根腐病的影响[J].北方园艺,2012,36(04):140.
CAO Li-xiang,SU Wei-guo,LUAN Ye-liang,et al.Effects of Different Medicament Treatment on Ficus carica L. Root Rot[J].Northern Horticulture,2012,36(14):140.
[3]赵春莉,李金英.黄花乌头根腐病菌的室内药剂筛选[J].北方园艺,2012,36(19):155.
ZHAO Chun-li,LI Jin-ying.Indoor Reagent Screening of Aconitum coreanum Root Rot Disease[J].Northern Horticulture,2012,36(14):155.
[4]周 莹,袁孟娟,韩 军,等.中药材丹参根腐病生防菌的分离与鉴定[J].北方园艺,2015,39(01):145.[doi:10.11937/bfyy.201501039]
ZHOU Ying,YUAN Meng-juan,HAN Jun,et al.Isolation and Identification of Traditional Chinese Medicine Salvia miltiorrhiza Bunge Root Rot Biocontrol Agents[J].Northern Horticulture,2015,39(14):145.[doi:10.11937/bfyy.201501039]
[5]程亮.青海省蚕豆品种（系）抗根腐病鉴定[J].北方园艺,2015,39(06):103.[doi:10.11937/bfyy.201506028]
CHENG Liang.Resistance Identification of Broad Bean Varieties (Lines)to Root Rot in Qinghai Province[J].Northern Horticulture,2015,39(14):103.[doi:10.11937/bfyy.201506028]
[6]李 雪,秦祎婷,李 耿,等.采收时期和生长年限对辽藁本产量和品质的影响[J].北方园艺,2015,39(11):152.[doi:10.11937/bfyy.201511039]
LI Xue,QIN Yi-ting,LI Geng,et al.Study on Yield and Quality of Ligusticum jeholense in Different Harvest Seasons and Growth Years[J].Northern Horticulture,2015,39(14):152.[doi:10.11937/bfyy.201511039]
[7]张会会,史娟,王俊,等.不同微生物菌剂和生物有机肥对黄芪地下生长量及根腐病的影响[J].北方园艺,2016,40(01):140.[doi:10.11937/bfyy.201601037]
ZHANG Huihui,SHI Juan,WANG Jun,et al.Effect of Different Microbial Agents and Biological Organic Fertilizer on Root Growth and Root Rot Underground of Astragalus[J].Northern Horticulture,2016,40(14):140.[doi:10.11937/bfyy.201601037]
[8]方晓翠,屈荷丽,赵思峰,等.新疆加工辣椒根腐病病原鉴定[J].北方园艺,2016,40(15):108.[doi:10.11937/bfyy.201615027]
FANG Xiaocui,QU Heli,ZHAO Sifeng,et al.Pathogen Identification of Processing Chili Pepper Root Rot Disease in Xinjiang[J].Northern Horticulture,2016,40(14):108.[doi:10.11937/bfyy.201615027]
[9]吴晓云,胡尊瑞,韩振芹,等.八种杀菌剂对草莓根腐病的室内毒力和田间防效评价[J].北方园艺,2020,44(17):49.[doi:10.11937/bfyy.20194265]
WU Xiaoyun,HU Zunrui,HAN Zhenqin,et al.Toxicity and Field Control Efficacy of Eight Chemical and Biological Fungicides on Strawberry Root Rot[J].Northern Horticulture,2020,44(14):49.[doi:10.11937/bfyy.20194265]
[10]杨瑞先,刘萍,汪玉婷,等.洛阳地区牡丹根腐病病原菌的分离与鉴定[J].北方园艺,2021,(01):59.[doi:10.11937/bfyy.20200399]
YANG Ruixian,LIU Ping,WANG Yuting,et al.Isolation and Identification of Root Rot Pathogens on Paeonia suffruticosa Andr. in Luoyang[J].Northern Horticulture,2021,(14):59.[doi:10.11937/bfyy.20200399]
备注/Memo
第一作者简介：张天静（1979-），女，本科，助理研究员，现主要从事中药材病原菌分离鉴定及病虫害防治等研究工作。Email：873613102@qq.com.责任作者：孙文松（1973-），男，本科，研究员，现主要从事中药材种质收集保护、品种选育及标准化栽培等研究工作。Email:sunwensong12@126.com.基金项目：国家中药材产业技术体系建设专项资金资助项目（CARS-21-06）；辽宁省农业科学院重大科技成果培育资助项目（2020CGPY0403）；辽宁省农业科学院学科建设计划资助项目(2019DD144221)。收稿日期：2022-12-23