XU Lei,LIU Changli,GUO Lanping.Soil Bacterial Diversity and Function Prediction Analysis of Poria cocos Cultivating Under Different Planting Modes[J].Northern Horticulture,2023,(11):91-97.[doi:10.11937/bfyy.20223163]
不同种植模式下茯苓土壤细菌多样性和功能预测分析
- Title:
- Soil Bacterial Diversity and Function Prediction Analysis of Poria cocos Cultivating Under Different Planting Modes
- 文献标志码:
- A
- 摘要:
- 以不同种植模式茯苓的栽培土壤为试材，采用Illumina NovaSeq高通量测序技术，研究茯苓林药仿生栽培和农田单作栽培2种模式，种植前和菌核采收后土壤细菌群落变化，并采用Tax4Fun进行宏基因组的功能预测，以期为茯苓适宜栽培模式及忌重茬发生机制提供参考依据。结果表明：2种模式栽培茯苓后，土壤细菌OTUs数少量增加。Alpha多样性分析显示，Shannon、Simpson、Chao1、〖JP2〗ACE指数均无显著差异。2种栽培模式相对丰度变化居前的门均为变形菌门、酸杆菌门、厚壁菌门、疣微菌门、泉古菌门、放线菌门、拟杆菌门、绿弯菌门、硝化螺旋菌门，农田单作栽培中上述各门相对丰度均无显著变化，林药仿生栽培中酸杆菌门、疣微菌门、泉古菌门3个门相对丰度显著增加，但未达极显著水平。2种模式相对丰度大于10%的优势菌群均为变形菌门、酸杆菌门。UPGMA聚类分析显示，2种栽培模式各自聚为一大类。Tax4Fun功能预测在第一等级上注释到6类功能，二级功能预测包含35项子功能。综上所述，不同模式栽培茯苓后细菌群落结构较稳定，细菌数量仅有少量增加，优势细菌群落无显著变化，土壤细菌或许不是导致茯苓不耐连作的主要原因。
- Abstract:
- Taking the cultivating soil of Poria cocos by different planting modes as experimental materials,using Illumina novaseq high-throughput sequencing technology to study the changes of soil bacterial community before planting and after sclerotium harvest of Poria cocos forest-medicine bionic cultivation and farmland monoculture cultivation.The function of metagenomewas predicted using Tax4fun,in order to provide reference for the suitable cultivation mode and the avoiding continuous cropping mechanism of Poria cocos.The results showed that the number of soil bacterial OTUs increased slightly after two cultivation modes of Poria cocos.Alpha diversity analysis showed that there was no significant difference among Shannon index,Simpson index,Chao1 index and ACE index.The phyla with the highest relative abundance were Proteobacteria,Acidobacteria,Firmicutes,Verrucomicrobia,Crenarchaeota,Actinobacteria,Bacteroidetes,Chloroflexi,Nitrospirae.There was no significant change in the relative abundance of the above-mentioned departments in the farmland monoculture cultivation.In forest-medicine bionic cultivation,the relative abundance of Acidobacteria Verrucomicrobia and Crenarchaeota increased significantly,but did not reach a very significant level.UPGMA cluster analysis showed that the two cultivation patterns were clustered into one category respectively.Tax4fun function prediction was annotated to 6 functions at the first level,the secondary function prediction included 35 sub functions.In summary,the bacterial community structure of Poria cocos cultivated in different models was relatively stable,the number of bacteria increased only a small amount,and the dominant bacterial community did not change significantly.Soil bacteria might not be the main cause of Poria cocos intolerance to continuous cropping.
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备注/Memo
第一作者简介：徐雷（1982-），男，博士，副教授，现主要从事中药资源及其品质等研究工作。E-mail：2310770630@qq.com.责任作者：郭兰萍（1969-），女，博士，研究员，现主要从事中药生态农业等研究工作。E-mail: glp01@126.com.收稿日期：2022-08-01