ZHAO Muqiu,ZHOU Nana,DU Qianjin,et al.Effect of Rice-vegetable Rotation on Soil Bacterial Community Under Straw Returning[J].Northern Horticulture,2018,42(14):109-117.[doi:10.11937/bfyy.20173901]
赵牧秋，周娜娜，杜前进，黎明，史云峰
- Title:
- Effect of Rice-vegetable Rotation on Soil Bacterial Community Under Straw Returning
- Keywords:
- bacterial community; high-throughput sequencing; soil; straw returning; rotation
- 文献标志码:
- A
- 摘要:
- 为了探明海南地区秸秆还田条件下水稻-蔬菜轮作对土壤细菌群落的影响，通过设置水稻-水稻（SS）、水稻-青瓜（SQ）和水稻-黄秋葵（SH）3个处理，经持续2年的田间试验，采用Illumina平台HiSeq〖JP+2〗高通量测序技术测定并比较了土壤细菌群落的差异，分析了其与土壤理化性质的关系。结果表明：1）测序共获得347 544条读数，平均长度436 bp，计16 094个OTUs。SS处理土壤含有的OTUs最多，分别较SQ和SH处理高出7.8%和9.4%。2）3个处理土壤细菌丰富度无显著（P<0.05）差异，水稻-青瓜轮作（SQ）会降低土壤细菌多样性，提高细菌优势度。3）水稻-蔬菜轮作使厚壁菌门（Firmicutes）和芽单胞菌门（Gemmatimonadetes）的相对丰度升高，绿弯菌门（Chloroflexi）和酸杆菌门（Acidobacteria）的相对丰度降低；增加了放线菌纲（Actinobacteria）、芽孢杆菌纲（Bacilli）、芽单胞菌纲（Gemmatimonadetes）、γ-变形菌纲（Gammaproteobacteria）和热微菌纲（Thermomicrobia）的相对丰度，降低了酸杆菌纲（Acidobacteria）、β-变形菌纲（Betaproteobacteria）和纤线杆菌纲（Ktedonobacteria）的相对丰度。4）变形菌门和放线菌门的相对丰度与土壤电导率（EC）和有效磷含量（AP）正相关，与C/N负相关；绿弯菌门和酸杆菌门的相对丰度与土壤pH、C/N正相关，与土壤有机质（OM）和碱解氮（AHN）含量负相关。
- Abstract:
- In order to investigate the influence of rice-vegetable rotation on soil bacterial community under straw returning in Hainan area，a field experiment was set including three treatments (rice-rice SS，rice-cucumber SQ and rice-okra SH) and lasted for two years.The differences of soil bacterial communities were determined by high-throughput sequencing method on Illumina HiSeq platform，and the relationship between soil bacterial communities and physical-chemical properties was analysed.The results showed that，1) 347 544 reads of 16 094 OTUs were generated under all the treatments with an average length of 436 bp.The soil of SS treatment contained the most OTUs，which were 7.8% and 9.4% more than that of SQ and SH treatments，respectively.2) There was no significant difference (P<0.05) in soil bacterial richness among three treatments.Rice-cucumber rotation (SQ) reduced the bacterial diversity of soil but increased the bacterial dominance.3) Rice-vegetable rotation increased the relative abundance of Firmicutes and Gemmatimonadetes，but decreased the relative abundance of Chloroflexi and Acidobacteria on phylum level，and increased the relative abundance of Actinobacteria，Bacilli，Gemmatimonadetes，Gammaproteobacteria and Thermomicrobia，decreased the relative abundance of Acidobacteria，Betaproteobacteria and Ktedonobacteria on class level.4) The relative abundance of Proteobacteria and Actinobacteria was positively correlated with soil electrical conductivity (EC) and available phosphorus content (AP)，but negatively correlated with soil C/N.While the relative abundance of Chloroflexi and Acidobacteria was positively correlated with soil pH and C/N，but negatively correlated with soil organic matter (OM) and alkali hydrolyzable nitrogen (AHN) content.
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备注/Memo
第一作者简介：赵牧秋（1983-），女，辽宁沈阳人，博士，教授，研究方向为农业生态学。E-mail：zhaomuqiu@sina.com.责任作者：史云峰（1981-），男，博士，教授，研究方向为土壤与植物营养学。E-mail：shiyunfeng8189@sina.com.基金项目：海南耕地改良关键技术研究与示范专项及其配套资助项目（HNGDgl201501，2015PT28）；三亚市农业科技创新资助项目（2014NK25）；海南省自然科学基金资助项目（20153109）。收稿日期：2018-02-28