ZHENG Tianjiao,WANG Mengjiao.Diversity and Differences of Rhizosphere Microbial Community Structure in Different Blueberries[J].Northern Horticulture,2022,(14):76-86.[doi:10.11937/bfyy.20220166]
不同蓝莓根际微生物群落结构多样性及其差异
- Title:
- Diversity and Differences of Rhizosphere Microbial Community Structure in Different Blueberries
- 文献标志码:
- A
- 摘要:
- 以陕西汉中定军山蓝莓基地兔眼蓝莓、南高丛蓝莓和北高丛蓝莓3种蓝莓根际土壤为试材,运用高通量扩增子测序技术,对土壤微生物的多样性、结构和组成进行分析,以期为后续开发蓝莓功能微生物提供参考依据。结果表明:不同品种的蓝莓根际微生物群落结构存在显著性差异,其中北高丛蓝莓土样细菌多样性最高,兔眼蓝莓根际土样真菌多样性最高。蓝莓根际土壤占比大于1%的细菌门共有11个,占比大于1%的细菌属共有20个,酸杆菌门、放线菌门和变形菌门为3种蓝莓根际共同优势细菌门。占比大于1%的真菌门有3个,占比大于1%的真菌属有14个,子囊菌门为3种蓝莓根际共同优势真菌门。品种类型是影响蓝莓根际微生物群落结构和多样性的重要因素。
- Abstract:
- Rhizosphere microorganisms diversity,structure and compositionof Vaccinium ashei,South Vaccinium corymbosum and North Vaccinium corymbosum in Dingjun Mountain,Hanzhong,Shaanxi Province were analyzed by high throughput amplicon sequencing method,in order to provide reference for the subsequent development of blueberry functional microorganisms.The results showed that there were significant differences in the microbial community structure in the rhizosphere of different blueberries.The diversity of rhizosphere bacteria of North Vaccinium corymbosum were the highest diversity.The diversity of rhizosphere fungi of Vaccinium ashei were the highest diversity.There were 11 bacterial phyla that account for more than 1% of blueberry rhizosphere soil,and 20 bacterial genera that account for more than 1%.Acidobacteria,Actinobacteria and Proteobacteria were the three common dominant bacteria phyla of blueberry rhizosphere.There were 3 fungal phyla that account for
参考文献/References:
[1]吴林.我国越橘栽培生理研究进展[J].吉林农业大学学报2013,35(4):379-383.[2]秦兴川.蓝莓适生环境与栽培技术研究[J].园艺与种苗,2012(4):37-39.[3]李丽敏,郝庆生,李亚东.中国蓝莓产业发展若干问题的思考[J].安徽农业科学,2008(20):8546-8548.[4]吴文勇.蓝莓的生物学特性及栽培技术[J].中国南方果树,2008,37(2):47-49.[5]邵春英,崔自亮,杨艳丽.蓝莓优良品种介绍[J].种业导刊,2007(8):36.[6]李亚东,唐雪东,袁菲,等.我国小浆果生产现状、问题和发展趋势[J].东北农业大学学报,2011,42(1):1-10.[7]刘王锁,李海泉,何毅,等.根际微生物对植物与土壤交互调控的研究进展[J].中国土壤与肥料,2021(5):318-327.[8]EDWARDS J,CAMERON J,SANTOS-MEDELLN C,et al.Structure,variation,and assembly of the root-associated microbiomes of rice[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(8):911-920.[9]FINKEL O M,CASTRILLO G,PAREDES S H,et al.Understanding and exploiting plant beneficial microbes-science direct[J].Current Opinion in Plant Biology,2017,38:155-163.[10]LU T,KE M,LAVOIE M,et al.Rhizosphere microorganisms can influence the timing of plant flowering[J].Microbiome,2018,6(1):231.[11]ACOSTA-MARTINEZ V,BUROW G,ZOBECK T M,et al.Soil microbial communities and function in alternative systems to continuous cotton[J].Soil Science Society of America Journal,2010,74(4):1181-1192.[12]HARDOIM P R,NAZIR R,SESSITSCH A,et al.The new species Enterobacter oryziphilus sp.nov.and Enterobacter oryzendophyticus sp.nov.are key inhabitants of the endosphere of rice[J].Bmc Microbiology,2013,13(1):164-164.[13]BOUFFAUD ML,POIRIER MA,MULLER D,et al.Root microbiome relates to plant host evolution in maize and other Poaceae[J].Environ Microbiol,2014,16(9):2804-2814.[14]ZIPFE L,OLDROY D,GE D.Plant signaling in symbiosis and immunity[J].Nature,2017,543(7645):328-336.[15]BULGARELLI D,GARRIDO-OTER R,P MNCH,et al.Structure and function of the bacterial root microbiota in wild and domesticated barley[J].Cell Host & Microbe,2015,17(3):392-403.[16]DALLA S O R,HERNNDEZ R F,ALVAREZ G L M,et al.Azospirillum sp.inoculation in wheat,barley and oats seeds greenhouse experiments[J].Brazilian Archives of Biology and Technology,2004,47(6):843-850.[17]SAITOU N,NEI M.The neighbor-joining method:A new method for reconstructing phylogenetic trees[J].Mol Biol Evol,1987(4):406-425.[18]彭剑华,詹潮安,肖泽鑫,等.汕头地区台湾相思人工次生林林下木本植物的物种多样性分布研究[J].西南师范大学学报(自然科学版),2013,38(3):87-93.[19]SCHIMEL D,MELILLO J M,TIAN H Q,et al.Contribution of increasing CO2 and climate to carbon storage by ecosystems in the United States[J].Science,2000,287(5460):2004-2006.[20]PRESTON M J,BODDY L,RANDERSON P F.Analysis of microbial community functional diversity using sole-carbon-source utilisationprofile:A critique[J].Fems Microbiol Ecol,2002,42(1):1-14.[21]纳小凡,郑国琦,彭励,等.不同种植年限宁夏枸杞根际微生物多样性变化[J].土壤学报,2016,53(1):241-252.[22]赵帆,赵密珍,王钰,等.基于高通量测序研究草莓根际微生物群落结构和多样性[J].土壤,2019,51(1):51-60.[23]PEIFFER J A,SPOR A,KOREN O,et al.Diversity and heritability of the maize rhizosphere microbiome under field conditions[J].Proceedings of the National Academy of Sciences,2013,110(16):6548-6553.[24]SPAIN A M,KRUMHOLZ L R,ELSHAHED M S.Abundance,composition,diversity and novelty of soil Proteobacteria[J].Isme Journal,2009,3(8):992-1000.[25]王君.蓝莓根际促生细菌的筛选、鉴定及其促生效果[D].泰安:山东农业大学,2016.[26]张典利,孟臻,亓文哲,等.植物根际促生菌的研究与应用现状[J].世界农药,2018,40(6):37-43,50.[27]赵吉,廖仰南.羊草草地分解者亚系统的特性及作用[J].生态学报,1995,15(4):359-364.[28]常强强,范佳佳,刘慧君,等.不同地区青檀根际土壤肥力和真菌多样性及其相关性分析[J].植物资源与环境学报,2017,26(3):27-34.[29]廖咏梅,黄元腾吉,邹承武,等.香蕉植株根区土壤的真菌多样性分析[J].广西植物,2020,40(1):99-107.[30]BEIMFORDE C,FELDBERG K,NYLINDER S,et al.Estimating the phanerozoic history of the ascomycota lineages:Combining fossil and molecular data[J].Molecular Phylogenetics and Evolution,2014,78:386-398.[31]陈林,张佳宝.玉米品种对根际微生物特性的影响[J].中国农学通报,2014,30(15):62-67.[32]周田田,丁榕,蔡建超,等.杜鹃根际微生物的分离、鉴定及多样性分析[J].上海农业学报,2017,33(2):63-70.[33]罗晓蔓,周书宇,杨雪.植物根系分泌物的分类和作用[J].安徽农业科学,2019,47(4):37-39,45.[34]高雪峰.短花针茅荒漠草原优势植物根系分泌物及其主要组分对土壤微生物的影响[D].呼和浩特:内蒙古农业大学,2017.[35]王小平,肖肖,唐天文,等.连香树人工林根系分泌物输入季节性变化及其驱动的根际微生物特性研究[J].植物研究,2018,38(1):47-55.[36]毛鹏娟.不同品种蓝莓在陕西的引种试验[D].杨凌:西北农林科技大学,2016.[37]田丹丹,周维,黄素梅,等.不同香蕉品种根系分泌物对香蕉枯萎病致病菌的影响及组成分析[J].热带作物学报,2017,38(5):910-914.
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备注/Memo
第一作者简介:郑天骄(1996-),男,硕士研究生,研究方向为植物根际土壤微生物。E-mail:1617652358@qq.com.责任作者:王梦姣(1987-),女,博士,副教授,现主要从事植物根际土壤微生物等研究工作。E-mail:amy133253@126.com.基金项目:陕西省科技厅青年基金资助项目(2021JQ-755);陕西省高校科协青年人才托举计划资助项目(20200206)。收稿日期:2022-01-13